Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry, CyTOF-ready

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # BU53
View all SLC7A5/LAT1 Primary Antibodies »

Product Specifications

Immunogen

Plasma cell line 8226
Accession # Q01650

Specificity

Detects Human SLC7A5/LAT1 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human SLC7A5/LAT1 Antibody

Detection of SLC7A5/LAT1 antibody in Raji Human Cell Line antibody by Flow Cytometry.

Detection of SLC7A5/LAT1 in Raji Human Cell Line by Flow Cytometry.

Raji human Burkitt lymphoma cell line was stained with Mouse Anti-Human SLC7A5/LAT1 Monoclonal Antibody (Catalog # MAB10390, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human SLC7A5/LAT1 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Raji human Burkitt lymphoma cell line

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SLC7A5/LAT1

Human Large neutral amino acids transporter small subunit 1, also known as SLC7A5, CD98 Light Chain (CD98LC), 4F2 light chain and LAT1 belongs to the APC (Amino acid-Polyamine-organo Cation) superfamily and forms a heterodimer with SLC3A2 to function as sodium-independent, high-affinity transporter that mediates uptake of large neutral amino acids such as phenylalanine, tyrosine, L-DOPA, leucine, histidine, methionine and tryptophan.

Long Name

Solute Carrier Family 7 Member 5

Alternate Names

4F2LC, CD98lc, E16, LAT1, MPE16, TA1

Entrez Gene IDs

8140 (Human); 20539 (Mouse); 50719 (Rat)

Gene Symbol

SLC7A5

UniProt

Q01650

Additional SLC7A5/LAT1 Products

Product Documents for Human SLC7A5/LAT1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human SLC7A5/LAT1 Antibody

For research use only

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