SMAC/Diablo in Human Prostate. SMAC/Diablo was detected in immersion fixed paraffin-embedded sections of human prostate using Goat Anti-Human SMAC/Diablo Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7891) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of glandular epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human SMAC/Diablo by Western Blot. Western blot shows lysates of SH‑SY5Y human neuroblastoma cell line, mouse NB41A3 mouse neuroblastoma cell line, and PC‑12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human SMAC/Diablo Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7891) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for SMAC/Diablo at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
SMAC (second mitochondria derived activator of caspase)/Diablo promotes caspase activation by interacting with the inhibitor of apoptosis (IAP) proteins in the cytochrome c/Apaf-1/caspase-9 pathway.
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Chai, J. et al. (2000) Nature 406:855.
Srinivasula, S. et al. (2000) J. Biol. Chem. 275:36152.
Ekert, P. et al. (2001) J. Cell Biol. 152:483.
Verhagen, A. (2000) Cell 102:43.
Second Mitochondria-derived Activator of Caspase
Entrez Gene IDs:
0610041G12Rik; Diablo; diablo, IAP-binding mitochondrial protein; Direct IAP-binding protein with low pI; mitochondrial Smac protein; Second mitochondria-derived activator of caspase; Smac; SMACmitochondrial
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