Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Smad5 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Smad5
Val155-Arg258
Accession # Q99717

Specificity

Detects human Smad5 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) Smad1, rhSmad2 and rhSmad4 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Smad5 Antibody

Smad5 antibody in Human Colon by Immunohistochemistry (IHC-P).

Smad5 in Human Colon.

Smad5 was detected in immersion fixed paraffin-embedded sections of human colon using 5 µg/mL Human Smad5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2250) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Smad5 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human colon

Western Blot

0.1 µg/mL
Sample: Recombinant Human Smad5

Reviewed Applications

Read 2 reviews rated 4.5 using AF2250 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Smad5

Smad5 (Smad; an amalgam of the Sma gene in worm and the MAD gene in fruitfly) is a transcriptional modulator that is activated by BMP receptor kinases. Human Smad5 shares 99% amino acid sequence homology with the mouse or rat protein.

Long Name

Mothers Against DPP Homolog 5

Alternate Names

Dwfc, JV5-1, MADH5

Entrez Gene IDs

4090 (Human)

Gene Symbol

SMAD5

UniProt

Q99717

Additional Smad5 Products

Product Documents for Human Smad5 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Smad5 Antibody

For research use only

Citations for Human Smad5 Antibody

Customer Reviews for Human Smad5 Antibody (2)

4.5 out of 5
2 Customer Ratings
5 Stars
50%
4 Stars
50%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Human Smad5 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 2 of 2 reviews Showing All
Filter By:
Clear Filters X
  • Human Smad5 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Embryo tissue
    Species: Human
    Verified Customer | Posted 11/28/2024
    Human Smad5 Antibody AF2250
  • Human Smad5 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Mv1Lu mink lung epithelial cell line
    Species: Mink
    Verified Customer | Posted 11/20/2019
    probe with Smad 5 antibody at 0.5ug/mL. I observed a weak band at the expected MW but there were many extra bands in my samples.
    Human Smad5 Antibody AF2250

There are no reviews that match your criteria.

Showing  1 - 2 of 2 reviews Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies