Human Smad5 Antibody Summary
Accession # Q99717
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Smad5 in Human Colon. Smad5 was detected in immersion fixed paraffin-embedded sections of human colon using 5 µg/mL Human Smad5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2250) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Smad5 (Smad; an amalgam of the Sma gene in worm and the MAD gene in fruitfly) is a transcriptional modulator that is activated by BMP receptor kinases. Human Smad5 shares 99% amino acid sequence homology with the mouse or rat protein.
Citation for Human Smad5 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Targeting of SMAD5 links microRNA-155 to the TGF-beta pathway and lymphomagenesis.
Authors: Rai D, Kim SW, McKeller MR
Proc. Natl. Acad. Sci. U.S.A., 2010;107(7):3111-6.
Sample Types: Whole Cells
Applications: Western Blot
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Cell and Tissue Staining Kits
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