Human Smad8 Antibody

Catalog # Availability Size / Price Qty
AF2309
AF2309-SP
Product Details
FAQs
Supplemental Products
Reviews (1)

Human Smad8 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Smad8 in direct ELISAs and Western blots. In direct ELISAs, approximately 90% cross-reactivity with recombinant (rh) Smad1 is observed, approximately 20% cross-reactivity with rhSmad4 is observed, and less than 1% cross-reactivity with rhSmad5, rhSmad7, and rhDNAK is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Smad8
Ala158-Ser233
Accession # NP_005896
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Smad8
Immunohistochemistry
5-15 µg/mL
Immersion fixed paraffin-embedded sections of human breast

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Smad8

Smads are a family of intracellular proteins that transmit transforming growth factor beta (TGF-beta ) superfamily signals from the cell surface to the nucleus. Smad8 was originally described as MADH6 and is also known as Smad9 and MADH9. Upon signaling by some BMP family members, Smad8 is phosphorylated resulting in its association with the common-mediator subunit, Smad4. This heteromeric complex then translocates into the nucleus to exert transcriptional comodulator activity.

Long Name
Mothers Against DPP Homolog 8
Entrez Gene IDs
4093 (Human)
Alternate Names
Smad8; Smad9

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human Smad8 Antibody

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Human Smad8 Antibody
By Anonymous on 11/20/2019
Application: WB Sample Tested: Mv1Lu mink lung epithelial cell line Species: Mink