Human SMAGP Antibody

Catalog # Availability Size / Price Qty
Detection of Human SMAGP by Western Blot.
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Product Details
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Human SMAGP Antibody Summary

Species Reactivity
Detects human SMAGP in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 40% cross-reactivity with recombinant rat SMAGP is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant human SMAGP
Thr2-Thr34, Phe56-Ile97
Accession # Q0VAQ4
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human SMAGP antibody by Western Blot. View Larger

Detection of Human SMAGP by Western Blot. Western blot shows lysates of human lung tissue, PC-3 human prostate cancer cell line, HT-29 human colon adenocarcinoma cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human SMAGP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3959) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for SMAGP at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunohistochemistry SMAGP antibody in Human Colon by Immunohistochemistry (IHC-P). View Larger

SMAGP in Human Colon. SMAGP was detected in immersion fixed paraffin-embedded sections of human colon using 15 µg/mL Goat Anti-Human SMAGP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3959) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SMAGP

SMAGP (small transmembrane and glycosylated protein) is a 25 kDa member of the SMAGP family of proteins. It is expressed on breast, colon and biliary duct epithelium, and likely plays a role in cell-cell adhesion. Human SMAGP is an O-glycosylated, 97 amino acid (aa) type III transmembrane glycoprotein that contains a signal sequenceless N-terminal extracellular region (aa 1-34) plus a 39 aa C-terminal cytoplasmic domain. Over the intra- and extracellular regions combined, human SMAGP is 76% aa identical to mouse SMAGP.

Long Name
Small Transmembrane and Glycosylated Protein
Entrez Gene IDs
57228 (Human); 207818 (Mouse); 300236 (Rat)
Alternate Names
hSMAGP; LOC57228; MGC149453; MGC149454; SMAGP; small cell adhesion glycoprotein; small cell transmembrane and glycosylated protein; Small transmembrane and glycosylated protein; small trans-membrane and glycosylated protein

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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