SMAGP in Human Colon.
SMAGP was detected in immersion fixed paraffin-embedded sections of human colon using 15 µg/mL Goat Anti-Human SMAGP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3959) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human SMAGP by Western Blot.
Western blot shows lysates of human lung tissue, PC‑3 human prostate cancer cell line, HT‑29 human colon adenocarcinoma cell line, and MCF‑7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human SMAGP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3959) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for SMAGP at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
SMAGP (small transmembrane and glycosylated protein) is a 25 kDa member of the SMAGP family of proteins. It is expressed on breast, colon and biliary duct epithelium, and likely plays a role in cell-cell adhesion. Human SMAGP is an O-glycosylated, 97 amino acid (aa) type III transmembrane glycoprotein that contains a signal sequenceless N-terminal extracellular region (aa 1-34) plus a 39 aa C-terminal cytoplasmic domain. Over the intra- and extracellular regions combined, human SMAGP is 76% aa identical to mouse SMAGP.
Small Transmembrane and Glycosylated Protein
Entrez Gene IDs:
57228 (Human); 207818 (Mouse); 300236 (Rat)
hSMAGP; LOC57228; MGC149453; MGC149454; SMAGP; small cell adhesion glycoprotein; small cell transmembrane and glycosylated protein; Small transmembrane and glycosylated protein; small trans-membrane and glycosylated protein
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