Human SOS2 Antibody Summary
Accession # Q07890
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Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human SOS2 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and 293T human embryonic kidney cell line. PVDF Membrane was probed with 2 µg/mL of Goat Anti-Human SOS2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6260) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for SOS2 at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
SOS2 (Son of sevenless homolog 2) is a 160‑170 kDa protein that regulates Ras signaling. It is widely expressed, and serves as a mediator of guanine nucleotide phosphate exchange. In the resting cell, cytoplasmic SOS2 forms a heterodimer with Grb2, and a heterotrimer with Eps8 and E3b1. Upon RTK activation, the SOS heterodimer is recruited to the cell membrane, where it contacts GDP‑bound Ras. This allows it to facilitate a GTP‑for‑GDP exchange that activates Ras. The heterotrimer interacts with Rac on actin filaments. SOS2 activity parallels that of SOS1. Relative to SOS1, however, SOS2 binds Grb2 with higher affinity and shows less biological activity due to a shorter half‑life. Human SOS2 is 1332 amino acids (aa) in length and contains one histone fold (aa 97‑169), PH (aa 439‑546) and REM (aa 595‑739) domains that interact with Ras, and a Pro‑rich region that binds to Grb2 (aa 1126‑1242).
Citation for Human SOS2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
BRAF activation by metabolic stress promotes glycolysis sensitizing NRASQ61-mutated melanomas to targeted therapy
Authors: K McGrail, P Granado-Ma, R Esteve-Pui, S García-Ort, Y Ding, S Sánchez-Re, B Ferrer, J Hernandez-, F Canals, A Manzano, A Navarro-Sa, R Bartrons, O Yanes, M Pérez-Alea, E Muñoz-Cous, V Garcia-Pat, JA Recio
Nature Communications, 2022;13(1):7113.
Sample Types: Whole Cells
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