Human SOX10 Antibody

Catalog # Availability Size / Price Qty
Detection of Human SOX10 by Western Blot.
2 Images
Product Details
Supplemental Products

Human SOX10 Antibody Summary

Species Reactivity
Detects human SOX10 in direct ELISAs.
Monoclonal Mouse IgG1 Clone # 1017727
Protein A or G purified from hybridoma culture supernatant
Synthetic human SOX10 peptide
Accession # P56693
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
2 µg/mL
See below
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human SOX10 antibody by Western Blot. View Larger

Detection of Human SOX10 by Western Blot. Western blot shows lysates of SK-Mel-28 human malignant melanoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human SOX10 Monoclonal Antibody (Catalog # MAB28643) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for SOX10 at approximately 49 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry SOX10 antibody in SK-Mel-28 Human Cell Line by Immunocytochemistry (ICC). View Larger

SOX10 in SK‑Mel‑28 Human Cell Line. SOX10 was detected in immersion fixed SK-Mel-28 human malignant melanoma cell line (positive stain) and U2OS human osteosarcoma cell line (negative stain) using Mouse Anti-Human SOX10 Monoclonal Antibody (Catalog # MAB28643) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SOX10

SOX10 belongs to the SOX family of transcription factors which display diverse roles during development. In the central nervous system, SOX10 is required for the terminal differentiation of oligodendrocytes and myelination. In the peripheral nervous system, SOX10 maintains pluripotency of neural crest stem cells and suppresses neuronal differentiation.

  1. Lo, L. et al. (2002) Development 129:1553.
Long Name
Transcription Factor SOX10
Entrez Gene IDs
6663 (Human)
Alternate Names
DOM; MGC15649; PCWH; SOX10; SRY (sex determining region Y)-box 10; SRY-related HMG-box gene 10; transcription factor SOX-10; WS4; WS4C; WS4mouse, human homolog of

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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