Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1017727
View all SOX10 Primary Antibodies »

Product Specifications

Immunogen

Synthetic human SOX10 peptide
Accession # P56693

Specificity

Detects human SOX10 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human SOX10 MAb (Clone 1017727)

Detection of Human SOX10 antibody by Western Blot.

Detection of Human SOX10 by Western Blot.

Western blot shows lysates of SK-Mel-28 human malignant melanoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human SOX10 Monoclonal Antibody (Catalog # MAB28643) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for SOX10 at approximately 49 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

SOX10 antibody in SK-Mel-28 Human Cell Line by Immunocytochemistry (ICC).

SOX10 in SK‑Mel‑28 Human Cell Line.

SOX10 was detected in immersion fixed SK-Mel-28 human malignant melanoma cell line (positive stain) and U2OS human osteosarcoma cell line (negative stain) using Mouse Anti-Human SOX10 Monoclonal Antibody (Catalog # MAB28643) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human SOX10 MAb (Clone 1017727)

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample:  Immersion fixed SK‑Mel‑28 human malignant melanoma cell line

Western Blot

2 µg/mL
Sample: SK‑Mel‑28 human malignant melanoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SOX10

SOX10 belongs to the SOX family of transcription factors which display diverse roles during development. In the central nervous system, SOX10 is required for the terminal differentiation of oligodendrocytes and myelination. In the peripheral nervous system, SOX10 maintains pluripotency of neural crest stem cells and suppresses neuronal differentiation.

References

  1. Lo, L. et al. (2002) Development 129:1553.

Long Name

Transcription Factor SOX10

Alternate Names

DOM, WS4

Entrez Gene IDs

6663 (Human)

Gene Symbol

SOX10

UniProt

P56693

Additional SOX10 Products

Product Documents for Human SOX10 MAb (Clone 1017727)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human SOX10 MAb (Clone 1017727)

For research use only

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Protocols

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FAQs

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