Human SOX13 Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB5118-100
MAB5118-SP
SOX13 in Human Pancreas.
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Human SOX13 Antibody Summary

Species Reactivity
Human
Specificity
Detects human SOX13 in direct ELISAs.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2559A
Purification
Protein A or G purified from cell culture supernatant
Immunogen
E. coli-derived recombinant human SOX13
Met19-Pro258
Accession # Q9UN79
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

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Applications

Recommended Concentration
Sample
Immunohistochemistry
3-25 µg/mL
Immersion fixed paraffin-embedded sections of human pancreas

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry SOX13 in Human Pancreas. View Larger

SOX13 in Human Pancreas. SOX13 was detected in immersion fixed paraffin-embedded sections of human pancreas using Rabbit Anti-Human SOX13 Monoclonal Antibody (Catalog # MAB5118) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei and cytoplasm. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SOX13

SOX-13 is a transcription factor involved in the embryonic development and the determination of cell fate.  It is also known as islet cell antibody 12 since it has been determined to be a type-1 diabetes autoantigen.  SOX-13 RNA is detected in most human tissues with highest levels in the pancreas, placenta, and kidney.  Immunohistochemistry on human pancreas identifies SOX-13 in the islets of Langerhans where staining is mostly cytoplasmic but can also be nuclear. SOX-13 antibody testing helps identify latent autoimmune diabetes of adults (LADA).

Long Name
SRY (sex determining region Y)-box 13
Entrez Gene IDs
9580 (Human); 20668 (Mouse); 289026 (Rat)
Alternate Names
ICA12; Islet cell antigen 12; MGC117216; SOX13; Sox-13; SRY (sex determining region Y)-box 13ICA12islet cell 12; SRY-box 13; SRY-related HMG-box gene 13; transcription factor SOX-13; Type 1 diabetes autoantigen ICA12

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