Human SOX17 Antibody (245013)

R&D Systems | Catalog # MAB1924

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Primate - Callithrix jacchus (Common Marmoset)

Applications

Validated:

Immunocytochemistry

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Clone # 245013
View all SOX17 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human SOX17
Asp177-Val414
Accession # Q9H6I2

Specificity

Detects human SOX17 in direct ELISAs. In direct ELISAs, this antibody does not cross-react with recombinant human SOX2, 3, 7, or 10.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3

Scientific Data Images for Human SOX17 Antibody (245013)

SOX17 antibody in B16 Mouse Cell Line by Immunocytochemistry (ICC).

SOX17 in B16 Mouse Cell Line.

SOX17 was detected in immersion fixed B16 mouse melanoma cell line using Mouse Anti-Human SOX17 Monoclonal Antibody (Catalog # MAB1924) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of SOX17 by Western Blot

Detection of SOX17 by Western Blot

VAMP5 KO doesn’t impair the stemness of ESCs.a–d Representative western blot images of VAMP5 KO potentially impair the related markers expression in hESCs (a) and also after differentiated into mesoderm (MES) (b), endoderm (END), (c) and ectoderm (ECT) (d). Data are represented as mean ± SEM, n = 3 biological replicates in each group. Student’s two tailed t test was used. No adjustments for multiple comparisons were made, as only two groups were compared. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. e PCA of the transcriptomic comparison of VAMP5 WT and KO in hESCs and followed differentiated three germ layers, n = 3 in each cell type. f Representative heatmap of hESCs and three germ layers related markers comparison of VAMP5 WT and KO in hESCs and followed differentiated three germ layers. g Immunofluorescence staining for lineage differentiation from VAMP5 WT and KO hESCs to mesoderm derived smooth muscles cells (markers of alpha -SMA and Transgelin), endoderm derived alveolar type II cells (markers of EpCAM and SP-B), and ectoderm derived neuron cells ( beta 3 tubulin and MAP2). The results are representative of three independent experiments. Scale bars, 50 μm. KO, knock-out; WT, wild-type. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40624080), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human SOX17 Antibody (245013)

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed human embryonic stem cell derived endodermal progenitors and B16 mouse melanoma cell line

Reviewed Applications

Read 4 reviews rated 5 using MAB1924 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SOX17

SOX17 is a member of the SOX family of transcription factors that bind DNA via a high mobility group (HMG) domain. SOX17 is suggested to play an important role in endoderm development (1, 2).

References

  1. Kanai-Azuma, M. et al. (2002) Development 129:2367.
  2. Katoh, M. et al. (2002) Int. J. Mol. Med. 9:153.

Long Name

Transcription Factor SOX17

Alternate Names

FLJ22252, SRY (sex determining region Y)-box 17, SRY-related HMG-box transcription factor SOX17, transcription factor SOX-17, VUR3

Entrez Gene IDs

64321 (Human)

Gene Symbol

SOX17

UniProt

Q9H6I2

Additional SOX17 Products

Product Documents for Human SOX17 Antibody (245013)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human SOX17 Antibody (245013)

For research use only

Citations for Human SOX17 Antibody (245013)

Customer Reviews for Human SOX17 Antibody (245013) (4)

5 out of 5
4 Customer Ratings
5 Stars
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4 Stars
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1 Stars
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Customer Images

Showing  1 - 4 of 4 reviews Showing All
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  • Human SOX17 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: MCF-7 human breast cancer cell line
    Species: Human
    Verified Customer | Posted 01/01/2026
    Whole cell lysate of human adipocytes and MCF7 coculture
    Human SOX17 Antibody (245013) MAB1924
  • Human SOX17 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HCT-116 human colorectal carcinoma cell line
    Species: Human
    Verified Customer | Posted 07/10/2025
    Human SOX17 Antibody (245013) MAB1924
    Bio-Techne Response
    This review reflects a new species or application tested on a primary antibody.
  • Human SOX17 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: iPS2 human induced pluripotent stem cells
    Species: Human
    Verified Customer | Posted 08/23/2021
    It worked well in 1:200 dilution
    Human SOX17 Antibody (245013) MAB1924
  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: See PMID 23041313
    Species: Mouse
    Verified Customer | Posted 02/12/2015

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