Human SOX17 Antibody MAB1924: R&D Systems

Human SOX17 Antibody

(6 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human SOX17 in direct ELISAs. In direct ELISAs, this antibody does not cross-react with recombinant human SOX2, 3, 7, or 10.
  • Source
    Monoclonal Mouse IgG3 Clone # 245013
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human SOX17
    Asp177-Val414
    Accession # Q9H6I2
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
SOX17 in B16 Mouse Cell Line. SOX17 was detected in immersion fixed B16 mouse melanoma cell line using Mouse Anti-Human SOX17 Monoclonal Antibody (Catalog # MAB1924) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SOX17
SOX17 is a member of the SOX family of transcription factors that bind DNA via a high mobility group (HMG) domain. SOX17 is suggested to play an important role in endoderm development (1, 2).
  • References:
    1. Kanai-Azuma, M. et al. (2002) Development 129:2367.
    2. Katoh, M. et al. (2002) Int. J. Mol. Med. 9:153.
  • Long Name:
    Transcription Factor SOX17
  • Entrez Gene IDs:
    64321 (Human)
  • Alternate Names:
    FLJ22252; SOX17; SRY (sex determining region Y)-box 17; SRY-related HMG-box transcription factor SOX17; transcription factor SOX-17; VUR3
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Species
Applications
Sample Type
  1. PI3K/mTORC2 regulates TGF-beta/Activin signalling by modulating Smad2/3 activity via linker phosphorylation.
    Authors: Yu J, Ramasamy T, Murphy N, Holt M, Czapiewski R, Wei S, Cui W
    Nat Commun, 2015;6(0):7212.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. Intrapatient variations in type 1 diabetes-specific iPS cell differentiation into insulin-producing cells.
    Authors: Thatava T, Kudva Y, Edukulla R, Squillace K, De Lamo J, Khan Y, Sakuma T, Ohmine S, Terzic A, Ikeda Y
    Mol Ther, 2013;21(1):228-39.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. FGF signaling via MAPK is required early and improves Activin A-induced definitive endoderm formation from human embryonic stem cells.
    Authors: Sui L, Mfopou J, Geens M, Sermon K, Bouwens L
    Biochem Biophys Res Commun, 2012;426(3):380-5.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  4. New cell lines from mouse epiblast share defining features with human embryonic stem cells.
    Authors: Tesar PJ, Chenoweth JG, Brook FA, Davies TJ, Evans EP, Mack DL, Gardner RL, McKay RD
    Nature, 2007;448(7150):196-9.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  5. Conditional gene expression in human embryonic stem cells.
    Authors: Vallier L, Alexander M, Pedersen R
    Stem Cells, 2007;25(6):1490-7.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  6. Neurogenic effect of vascular endothelial growth factor during germ layer formation of human embryonic stem cells.
    Authors: Kim BK, Kim SE, Shim JH, Woo DH, Gil JE, Kim SK, Kim JH
    FEBS Lett., 2006;580(25):5869-74.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
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