Human SOX17 Antibody

Catalog # Availability Size / Price Qty
MAB1924
MAB1924-SP
Human SOX17 Antibody in Immunocytochemistry
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Product Details
Citations (10)
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Human SOX17 Antibody Summary

Species Reactivity
Human
Specificity
Detects human SOX17 in direct ELISAs. In direct ELISAs, this antibody does not cross-react with recombinant human SOX2, 3, 7, or 10.
Source
Monoclonal Mouse IgG3 Clone # 245013
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human SOX17
Asp177-Val414
Accession # Q9H6I2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry SOX17 in B16 Mouse Cell Line. View Larger

SOX17 in B16 Mouse Cell Line. SOX17 was detected in immersion fixed B16 mouse melanoma cell line using Mouse Anti-Human SOX17 Monoclonal Antibody (Catalog # MAB1924) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SOX17

SOX17 is a member of the SOX family of transcription factors that bind DNA via a high mobility group (HMG) domain. SOX17 is suggested to play an important role in endoderm development (1, 2).

References
  1. Kanai-Azuma, M. et al. (2002) Development 129:2367.
  2. Katoh, M. et al. (2002) Int. J. Mol. Med. 9:153.
Long Name
Transcription Factor SOX17
Entrez Gene IDs
64321 (Human)
Alternate Names
FLJ22252; SOX17; SRY (sex determining region Y)-box 17; SRY-related HMG-box transcription factor SOX17; transcription factor SOX-17; VUR3

Product Datasheets

Citations for Human SOX17 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Measles vector as a multiple genes delivery platform facilitating iPSC reprogramming
    Authors: Q Wang, A Vossen, Y Ikeda, P Devaux
    Gene Ther., 2019;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. Generation of an induced pluripotent stem cell line from a patient with adult-onset leukoencephalopathy with axonal spheroids and pigmented glia (ALSP): HIHCNi003-A
    Authors: SN Hayer, Y Schelling, P Hoeflinger, S Hauser, L Schöls
    Stem Cell Res, 2018;30(0):206-209.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  3. Low Cell-Matrix Adhesion Reveals Two Subtypes of Human Pluripotent Stem Cells
    Authors: L Yu, J Li, J Hong, Y Takashima, N Fujimoto, M Nakajima, A Yamamoto, X Dong, Y Dang, Y Hou, W Yang, I Minami, K Okita, M Tanaka, C Luo, F Tang, Y Chen, C Tang, H Kotera, L Liu
    Stem Cell Reports, 2018;11(1):142-156.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  4. Generation of two human isogenic iPSC lines from fetal dermal fibroblasts
    Authors: R Tandon, B Brändl, N Baryshniko, A Landshamme, L Steenpa beta, O Keminer, O Pless, FJ Müller
    Stem Cell Res, 2018;33(0):120-124.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  5. PI3K/mTORC2 regulates TGF-beta/Activin signalling by modulating Smad2/3 activity via linker phosphorylation.
    Authors: Yu J, Ramasamy T, Murphy N, Holt M, Czapiewski R, Wei S, Cui W
    Nat Commun, 2015;6(0):7212.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Intrapatient variations in type 1 diabetes-specific iPS cell differentiation into insulin-producing cells.
    Authors: Thatava T, Kudva Y, Edukulla R, Squillace K, De Lamo J, Khan Y, Sakuma T, Ohmine S, Terzic A, Ikeda Y
    Mol Ther, 2013;21(1):228-39.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. FGF signaling via MAPK is required early and improves Activin A-induced definitive endoderm formation from human embryonic stem cells.
    Authors: Sui L, Mfopou J, Geens M, Sermon K, Bouwens L
    Biochem Biophys Res Commun, 2012;426(3):380-5.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  8. Conditional gene expression in human embryonic stem cells.
    Authors: Vallier L, Alexander M, Pedersen R
    Stem Cells, 2007;25(6):1490-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  9. New cell lines from mouse epiblast share defining features with human embryonic stem cells.
    Authors: Tesar PJ, Chenoweth JG, Brook FA, Davies TJ, Evans EP, Mack DL, Gardner RL, McKay RD
    Nature, 2007;448(7150):196-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  10. Neurogenic effect of vascular endothelial growth factor during germ layer formation of human embryonic stem cells.
    Authors: Kim BK, Kim SE, Shim JH, Woo DH, Gil JE, Kim SK, Kim JH
    FEBS Lett., 2006;580(25):5869-74.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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