Human Spinesin Antibody

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Product Details
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Human Spinesin Antibody Summary

Species Reactivity
Detects human Spinesin in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Spinesin is observed.
Monoclonal Mouse IgG2B Clone # 376608
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant human Spinesin
Accession # Q0P514
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

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Recommended Concentration
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Spinesin (Catalog # 2495‑SE), see our available Western blot detection antibodies
Measured by its ability to neutralize Recombinant Human Spinesin (1 µg/mL, Catalog # 2495-SE) cleavage of the fluorogenic peptide substrate Boc-QAR-Amc (0.1 mM, Catalog # ES014). The Neutralization Dose (ND50) is typically 7 µg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Spinesin

Spinesin, encoded by the TMPRSS5 gene, is a member of type II transmembrane serine proteases (TTSPs) (1). Human Spinesin contains the following structural domains: a short N‑terminal cytoplasmic tail (amino acid residues 1‑49), a transmembrane domain (residues 50‑70), a stem region and a serine protease domain (residues 71‑457) (2). The domain structure of Spinesin is common to other TTSPs, many of which have additional domains. The stem region of Spinesin contains a scavenger receptor-like domain. The ectodomain of human Spinesin (residues 71‑457) was expressed and purified as a single chain pro-enzyme. The deduced amino acid sequence contains a Leu instead of a Phe residue at position 369; the former is identical to the mouse protein (3, 4). The pro-enzyme can be activated and the resulting enzyme activity can be measured as described in the Activity Assay Protocol.

  1. Hooper, J.D. et al. (2001) J. Biol. Chem. 276:857.
  2. Yamaguchi, Y. et al. (2002) J. Biol. Chem. 277:6806.
  3. Carninci, P. et al. (2000) Genome Res. 10:1617.
  4. Shibata, K. et al. (2000) Genome Res. 10:1757.
Entrez Gene IDs
80975 (Human); 80893 (Mouse)
Alternate Names
EC 3.4.21; EC 3.4.21.-; EC; MGC141886; MGC148044; Spinesin; TMPRSS5; transmembrane protease serine 5; transmembrane protease, serine 5

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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