Human ST6 GalNAc alpha -2,6-sialyltransferase V/ST6GALNA Antibody

    
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  • Species Reactivity
    Human
  • Specificity
    Detects human GalNAc alpha -2,6-sialyltransferase V/ST6GALNAC5 in direct ELISAs. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) ST6GALNAC4 or rhST6GALNAC6 is observed.
  • Source
    Monoclonal Mouse IgG2A Clone # 719526
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human GalNAc alpha -2,6-sialyltransferase V/ST6GALNAC5
    Gly30-Phe336
    Accession # Q9BVH7
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of Human GalNAc  alpha ‑2,6 sialyltransferase V/
ST6GALNAC5 by Western Blot.

Western blot shows lysates of MDA‑MB‑231 human breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human GalNAc  alpha ‑2,6‑sialyltransferase V/ST6GALNAC5 Monoclonal Antibody (Catalog # MAB6715) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for GalNAc alpha -2,6-sialyltransferase V/ST6GALNAC5 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ST6 GalNAc alpha-2,6-sialyltransferaseV/ST6GALNAC5
Gangliosides are acidic glycosphingolipids that contain one or more sialic acid residues (1). They are abundant in the nervous system, where they play crucial modulatory roles in cellular recognition, interaction, adhesion, and signal transduction, particularly during early developmental stages. The expression of gangliosides in the nervous system is developmentally regulated through various sialyltransferases (2). ST6GALNAC5 is a sialyltransferase involved in the biosynthesis of ganglioside GD1a (NeuAc alpha 2,3Gal beta 1,3GalNAc beta 1,4(NeuAc alpha 2,3)Gal beta 1,4Glc beta 1-Cer) from GM1b (NeuAc alpha 2,3Gal beta 1,3GalNAc beta 1,4Gal beta 1,4Glc beta 1-Cer), and its expression is restricted to the brain (3, 4). ST6GALNAC5 has been identified as a key player in the metastasis of breast cancer cells to the brain by potentially enabling the cancer cells to cross the blood-brain barrier (5, 6). The recombinant ST6GALNAC5 was active on fetuin from fetal calf serum when assayed using a phosphatase-coupled method (7) suggesting the substrate specificity of ST6GALNAC5 may require further characterization.
  • References:
    1. Kolter, T. et al. (2002) J. Biol. Chem. 277:25859.
    2. Yu, R.K. et al. (2008) Glycoscience DOI: 10.1007/978-3-540-30429-6_41.
    3. Okajima, T. et al. (1999) J. Biol. Chem.274:30557.
    4. Harduin-Lepers, A. et al. (2005) Glycobiology 15:805.
    5. Bos, P.D. et al. (2009) Nature 459:1005.
    6. Arshad, F. et al. (2011). Patholog. Res. Int. DOI: 10.4061/2011/920509.
    7. Wu, Z.L. et al. (2010) Glycobiology DOI: 10.1093/glycob/cwq187.
  • Long Name:
    ST6 alpha-N-acetylGalactosaminide alpha-2,6-sialyltransferase 5
  • Entrez Gene IDs:
    81849 (Human); 26938 (Mouse)
  • Alternate Names:
    alpha-N-acetylneuraminyl 2,3-betagalactosyl-1,3)-N-acetyl galactosaminidealpha-2,6-sialyltransferase E; EC 2.4.99; EC 2.4.99.-; EC 2.4.99.7; GalNAc alpha-2,6-sialyltransferase V; GD1 alpha synthase; MGC3184; sialyltransferase 7 (alpha-N-acetylneuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase) E; sialyltransferase 7((alpha-N-acetylneuraminyl-2,3-beta-galactosyl-1,3)-N-acetyl galactosaminidealpha-2,6-sialyltransferase) E; Sialyltransferase 7E; SIAT7E; SIAT7-E; SIAT7Ealpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase V; ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminidealpha-2,6-sialyltransferase 5; ST6 neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminidealpha-2,6-sialyltransferase 5; ST6GalNAc V; ST6GALNAC5; ST6GalNAcV; ST6GalNAcValpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase 5
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Isotype Controls
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Mouse IgG2A Isotype Control

Ctrl MAB003 35  
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Mouse IgG2A Isotype Control

Ctrl MAB0031 7  
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Mouse IgG2A Isotype Control

Ctrl MAB003R
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Reconstitution Buffers
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Reconstitution Buffer 1 (PBS)

RB01
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 32  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 12  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow , IHC , ICC NL007 10
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow , IHC , ICC NL008 8
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 4  
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG Antibody

WB AF007 4
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow , IHC , ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Rat Anti-Mouse IgG2A PE-conjugated Antibody

Flow F0129 1  
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Mouse IgG2A Antibody

WB , Flow MAB0033  
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Rat Anti-Mouse IgG2A APC-conjugated Antibody

Flow F0130  
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Mouse IgG2A Antibody

Flow MAB0032  
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Rat Anti-Mouse IgG2A PerCP-conjugated Antibody

Flow F0131  
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