Detects human GalNAc alpha -2,6-sialyltransferase V/ST6GALNAC5 in direct ELISAs. In direct ELISAs and Western blots, no
cross-reactivity with recombinant human (rh) ST6GALNAC4 or rhST6GALNAC6 is
Monoclonal Mouse IgG2A Clone # 719526
Protein A or G purified from hybridoma culture supernatant
Chinese hamster ovary cell line CHO-derived recombinant human GalNAc alpha -2,6-sialyltransferase V/ST6GALNAC5 Gly30-Phe336 Accession # Q9BVH7
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human GalNAc alpha ‑2,6 sialyltransferase V/ ST6GALNAC5 by Western Blot.
Western blot shows lysates of MDA‑MB‑231 human breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human GalNAc alpha ‑2,6‑sialyltransferase V/ST6GALNAC5 Monoclonal Antibody (Catalog # MAB6715) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for GalNAc alpha -2,6-sialyltransferase V/ST6GALNAC5 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Gangliosides are acidic glycosphingolipids that contain one or more sialic acid residues (1). They are abundant in the nervous system, where they play crucial modulatory roles in cellular recognition, interaction, adhesion, and signal transduction, particularly during early developmental stages. The expression of gangliosides in the nervous system is developmentally regulated through various sialyltransferases (2). ST6GALNAC5 is a sialyltransferase involved in the biosynthesis of ganglioside GD1a (NeuAc alpha 2,3Gal beta 1,3GalNAc beta 1,4(NeuAc alpha 2,3)Gal beta 1,4Glc beta 1-Cer) from GM1b (NeuAc alpha 2,3Gal beta 1,3GalNAc beta 1,4Gal beta 1,4Glc beta 1-Cer), and its expression is restricted to the brain (3, 4). ST6GALNAC5 has been identified as a key player in the metastasis of breast cancer cells to the brain by potentially enabling the cancer cells to cross the blood-brain barrier (5, 6). The recombinant ST6GALNAC5 was active on fetuin from fetal calf serum when assayed using a phosphatase-coupled method (7) suggesting the substrate specificity of ST6GALNAC5 may require further characterization.
Kolter, T. et al. (2002) J. Biol. Chem. 277:25859.
Yu, R.K. et al. (2008) Glycoscience DOI: 10.1007/978-3-540-30429-6_41.
Okajima, T. et al. (1999) J. Biol. Chem.274:30557.
Harduin-Lepers, A. et al. (2005) Glycobiology 15:805.
Bos, P.D. et al. (2009) Nature 459:1005.
Arshad, F. et al. (2011). Patholog. Res. Int. DOI: 10.4061/2011/920509.
Wu, Z.L. et al. (2010) Glycobiology DOI: 10.1093/glycob/cwq187.
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