Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 418714
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Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human STAMP2
Met1-His459
Accession # EAW76907

Specificity

Detects human STAMP2-transfected NS0 cells but not irrelevant transfectants in flow cytometry.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human STAMP2 Antibody

Detection of STAMP2 antibody in Human STAMP2 Transfected HEK293 Human Cell Line antibody by Flow Cytometry.

Detection of STAMP2 in Human STAMP2 Transfected HEK293 Human Cell Line by Flow Cytometry.

Human STAMP2 transfected HEK293 human cell line was stained with Mouse Anti-Human STAMP2 Monoclonal Antibody (Catalog # MAB4626, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Applications for Human STAMP2 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: Human STAMP2 transfected HEK293 human cell line

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.


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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: STAMP2/STEAP4

Human STAMP2, also called Steap4 or TIARP (TNF-alpha -induced adipose-related protein), is a 55 kDa, 459 aa, 6-transmembrane (TM) member of the Steap family of metalloreductases. It is induced by androgens in androgen-regulated prostate cells, and is up‑regulated during adipocyte differentiation by TNF-alpha. Full-length human and mouse STAMP2 share 80% aa identity. A 283 aa variant lacks a portion of the oxidoreductase domain and three TM domains. STAMP2 is present in the Golgi complex and early endosomes in addition to the cell surface.

Long Name

Six Transmembrane Protein of Prostate 2

Alternate Names

STEAP4, TIARP, TNFAIP9

Entrez Gene IDs

79689 (Human); 117167 (Mouse)

Gene Symbol

STEAP4

UniProt

Additional STAMP2/STEAP4 Products

Product Documents for Human STAMP2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human STAMP2 Antibody

For research use only

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