Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 987420
View all SVEP1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide containing human SVEP1 Peptide
Accession # Q4LDE5

Specificity

Detects human SVEP1 in direct Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human SVEP1 Antibody

Detection of Human SVEP1 antibody by Western Blot.

Detection of Human SVEP1 by Western Blot.

Western blot shows lysates of MG-63 human osteosarcoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human SVEP1 Monoclonal Antibody (Catalog # MAB97741) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for SVEP1 at approximately 460 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Applications for Human SVEP1 Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: MG‑63 human osteosarcoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SVEP1

Sushi, von Willebrand factor type A, EGF and pentraxin domain-containing protein 1 (also known as Polydom), is a 3571 aminoacids (aa), 390KDa extracellular matrix protein encoded by the SVEP1 gene. SVEP1 has been identified as a multidomain cell adhesion protein that is a ligand for integrin alpha 9 beta 1. SVEP1 has been proposed to play a crucial role in epidermis differentiation and lymphatic remodeling.

Long Name

Sushi, Von Willebrand Factor Type A, EGF And Pentraxin Domain Containing 1

Alternate Names

C9orf13, CCP22, POLYDOM, SEL-OB, SELOB

Entrez Gene IDs

79987 (Human); 64817 (Mouse); 685899 (Rat)

Gene Symbol

SVEP1

UniProt

Q4LDE5

Additional SVEP1 Products

Product Documents for Human SVEP1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human SVEP1 Antibody

For research use only

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