Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all TAZ/WWTR1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human TAZ/WWTR1
Met267-Leu400
Accession # Q9GZV5

Specificity

Detects human TAZ/WWTR1 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human TAZ/WWTR1 Antibody

Detection of Human TAZ/WWTR1 antibody by Western Blot.

Detection of Human TAZ/WWTR1 by Western Blot.

Western blot shows lysates of A431 human epithelial carcinoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human TAZ/WWTR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7210) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TAZ/WWTR1 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human TAZ/WWTR1 Antibody

Application
Recommended Usage

Western Blot

0.2 µg/mL
Sample: A431 human epithelial carcinoma cell line and HeLa human cervical epithelial carcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TAZ/WWTR1

TAZ (Transcriptional co-Activatorr with PDZ-binding motif; also WWTR1) is a 50-55 kDa protein that is related to YAP65 TEF-1 interacting protein. It is a widely expressed transcriptional coactivator, and should not be confused with tafazzin/Taz, an enzyme associated with lipid metabolism. TAZ influences the nuclear transport of SMAD-2, -3 and -4, and in the nucleus, TAZ is known to interact with transcription factors such as TFF1, Pax8, NKX2-1 and TEADS, serving as a scaffold for transcriptional activation complexes. Human TAZ is 400 amino acids (aa) in length. It contains one WW domain (aa 124-157) that binds to PPXY motifs, a coiled-coil region (aa 225-259), and a PDZ binding domain (aa 394-400). There are at least four utilized phosphorylation sites. When phosphorylated on Ser89, TAZ preferentially bind to 14-3-3 proteins, promoting its retention in the cytoplasm. Over aa 267-400, human TAZ shares 88% aa identity with mouse TAZ.

Long Name

Transcriptional Coactivator with PDZ-binding Motif

Alternate Names

WWTR1

Entrez Gene IDs

25937 (Human); 97064 (Mouse); 295062 (Rat)

Gene Symbol

WWTR1

UniProt

Q9GZV5 (Human)

Additional TAZ/WWTR1 Products

Product Documents for Human TAZ/WWTR1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human TAZ/WWTR1 Antibody

For research use only

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