|TCL1A in Daudi Human Burkitt's Lymphoma Cells. TCL1A was detected in immersion fixed Daudi human Burkitt's lymphoma cell line using 10 µg/mL Goat Anti-Human TCL1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4847) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.|
|Detection of Human TCL1A by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line and Ramos human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human TCL1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4847) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, recombinant human TCL1A was included. A specific band for TCL1A was detected at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.|
TCL1A (T cell leukemia protein 1A; also p14 TCL1) is a 14 kDa member of the TCL1 family of proteins. It is expressed by thymocytes and activated T and B lymphocytes, and interacts with Akt kinase, promoting its activation and the subsequent deactivation of proapoptotic molecules. Human TCL1A is 114 amino acids (aa) in length. The amino acids Asp16 and Ile74 mediate Akt association, while Pro36 Leu37 Thr38 mediates TCL1A homodimerization. Full length human TCL1A is 50% aa identical to mouse TCL1A.
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