Human TCL1A Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human TCL1A in Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human TCL1A
    Met1-Asp114
    Accession # P56279
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
TCL1A in Daudi Human Burkitt's Lymphoma Cells. TCL1A was detected in immersion fixed Daudi human Burkitt's lymphoma cell line using 10 µg/mL Goat Anti-Human TCL1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4847) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human TCL1A by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line and Ramos human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human TCL1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4847) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, recombinant human TCL1A was included. A specific band for TCL1A was detected at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TCL1A

TCL1A (T cell leukemia protein 1A; also p14 TCL1) is a 14 kDa member of the TCL1 family of proteins. It is expressed by thymocytes and activated T and B lymphocytes, and interacts with Akt kinase, promoting its activation and the subsequent deactivation of proapoptotic molecules. Human TCL1A is 114 amino acids (aa) in length. The amino acids Asp16 and Ile74 mediate Akt association, while Pro36 Leu37 Thr38 mediates TCL1A homodimerization. Full length human TCL1A is 50% aa identical to mouse TCL1A.

  • Long Name:
    T Cell Leukemia Protein 1A
  • Entrez Gene IDs:
    8115 (Human); 21432 (Mouse); 690575 (Rat)
  • Alternate Names:
    Oncogene TCL1; Oncogene TCL-1; Protein p14 TCL1; T-cell leukemia/lymphoma 1A; T-cell lymphoma-1; T-cell lymphoma-1A; TCL1A; TCL1-PEN; TCL1T-cell leukemia/lymphoma protein 1A
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