Western blot shows lysates of human heart tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Tenascin X Monoclonal Antibody (Catalog # MAB6999) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Tenascin X at approximately 470 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Detection of Human Tenascin X by Simple WesternTM.
Simple Western lane view shows lysates of human heart tissue, loaded at 0.5 mg/mL. A specific band was detected for Tenascin X at approximately 376 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Tenascin X Monoclonal Antibody (Catalog # MAB6999). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Tenascin X
Tenascin XB2 (TN-XB2; also TN-XB-Short/S and tenascin-X isoform 2) is a 73-75 kDa member of the tenascin family of extracellular matrix molecules. It is expressed by adrenal gland, and represents the use of an intronic promoter that is tied to an internal start site within the TN-XB gene. A duplication of the TN-XB2 region has created a gene called TN-XA that codes for the same ORF but is not translated. Although TN-XB2 has been reported to bind to tropoelastin, plus collagens I, III and V, other studies using soluble fragments of TN-XB report only tropoelastin interaction. Human TN-XB2 is 673 amino acids (aa) in length. It contains four fibronectin type III repeats (aa 82-442), and one C-terminal fibrinogen-like globular region (aa 449-664). Antibodies to TN-XB2 recognize full-length 465 kDA Tenascin X (TN-XB), 75 kDa and 140-150 kDa C-terminal fragments. Although human TN-XB2 shares 84% aa identity with full-length mouse TN-XB in its C-terminus, there does not appear to be an equivalent alternative start site in the mouse gene.
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