Human Tenascin X Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Tenascin XB2 in ELISAs and Western blots. Also detects Tenascin X in Western blots. In direct ELISAs, no cross-reactivity with recombinant human Tenascin R is observed.
  • Source
    Monoclonal Mouse IgG2A Clone # 766842
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human Tenascin XB2
    Met1-Tyr673
    Accession # AAH33740
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Simple Western
    10 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples

Detection of Human Tenascin X by Western Blot. Western blot shows lysates of human heart tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Tenascin X Monoclonal Antibody (Catalog # MAB6999) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Tenascin X at approximately 470 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Detection of Human Tenascin X by Simple WesternTM. Simple Western lane view shows lysates of human heart tissue, loaded at 0.5 mg/mL. A specific band was detected for Tenascin X at approximately 376 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Tenascin X Monoclonal Antibody (Catalog # MAB6999). This experiment was conducted under reducing conditions and using the
66-440 kDa separation system.

Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Tenascin X

Tenascin XB2 (TN-XB2; also TN-XB-Short/S and tenascin-X isoform 2) is a 73-75 kDa member of the tenascin family of extracellular matrix molecules. It is expressed by adrenal gland, and represents the use of an intronic promoter that is tied to an internal start site within the TN-XB gene. A duplication of the TN-XB2 region has created a gene called TN-XA that codes for the same ORF but is not translated. Although TN-XB2 has been reported to bind to tropoelastin, plus collagens I, III and V, other studies using soluble fragments of TN-XB report only tropoelastin interaction. Human TN-XB2 is 673 amino acids (aa) in length. It contains four fibronectin type III repeats (aa 82-442), and one C-terminal fibrinogen-like globular region (aa 449-664). Antibodies to TN-XB2 recognize full-length 465 kDA Tenascin X (TN-XB), 75 kDa and 140-150 kDa C-terminal fragments. Although human TN-XB2 shares 84% aa identity with full-length mouse TN-XB in its C-terminus, there does not appear to be an equivalent alternative start site in the mouse gene.

  • Entrez Gene IDs:
    7148 (Human); 81877 (Mouse); 415089 (Rat)
  • Alternate Names:
    Hexabrachion-like protein; HXBL; Tenascin X; tenascin XB; tenascin XB2; tenascin-X; TNX; TNXB; TNXB1growth-inhibiting protein 45; TNXB2tenascin XB1; TNXBS; TNXBSTNXTENX; XB; XBS; XB-S; XBTN-X
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2A Isotype Control

Ctrl MAB003 32  
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Mouse IgG2A Isotype Control

Ctrl MAB0031 7  
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Mouse IgG2A Isotype Control

Ctrl MAB003R
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse IgG Antibody

WB AF007 4
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Rat Anti-Mouse IgG2A PE-conjugated Antibody

Flow F0129 1  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Rat Anti-Mouse IgG2A PerCP-conjugated Antibody

Flow F0131  
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Rat Anti-Mouse IgG2A APC-conjugated Antibody

Flow F0130  
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Mouse IgG2A Antibody

Flow MAB0032  
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