Human Tensin 4/CTEN Antibody

Catalog # Availability Size / Price Qty
MAB6925
MAB6925-SP
Human Tensin 4/CTEN Antibody in Immunohistochemistry
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Product Details
Citations (1)
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Human Tensin 4/CTEN Antibody Summary

Species Reactivity
Human
Specificity
Detects human Tensin 4/CTEN in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG1 Clone # 684524
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Tensin 4/CTEN
Ser2-Gln107
Accession # Q8IZW8
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line
Simple Western
10 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunohistochemistry Detection of Human Tensin 4/CTEN by Western Blot. View Larger

Detection of Human Tensin 4/CTEN by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Tensin 4/CTEN Monoclonal Antibody (Catalog # MAB6925) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Tensin 4/CTEN at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Tensin 4/CTEN in Human Prostate. View Larger

Tensin 4/CTEN in Human Prostate. Tensin 4/CTEN was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human Tensin 4/ CTEN Monoclonal Antibody (Catalog # MAB6925) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western Detection of Human Tensin 4/CTEN by Simple Western<sup>TM</sup>. View Larger

Detection of Human Tensin 4/CTEN by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Tensin 4/CTEN at approximately 74 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Tensin 4/CTEN Monoclonal Antibody (Catalog # MAB6925). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Tensin 4/CTEN

CTEN, also known as Tensin 4, is a 90 kDa cytoplasmic protein that is associated with focal adhesions in epithelial cells and is upregulated in several carcinomas. It promotes tumorigenesis by disrupting the attachment of focal adhesions to the cytoskeleton, promoting epithelial-mesenchymal transition, and binding nuclear beta‑Catenin. CTEN is cleaved by Caspase-3 at Asp570-Ser571 during apoptosis. It contains a serine-rich region (aa 168-324), one SH2 domain (aa 449-556), and a phosphatase domain (aa 548-714). Within aa 1-107, human CTEN shares 62% and 59% aa sequence identity with mouse and rat CTEN, respectively.

Entrez Gene IDs
84951 (Human); 217169 (Mouse); 303517 (Rat)
Alternate Names
CTEN; CTENC terminal tensin like; C-terminal tensin-like protein; FLJ14950; Tensin 4; tensin-4; TNS4

Product Datasheets

Citation for Human Tensin 4/CTEN Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. C-terminal tensin-like protein is a novel prognostic marker for primary melanoma patients.
    Authors: Sjoestroem C, Khosravi S, Zhang G, Martinka M, Li G
    PLoS ONE, 2013;8(11):e80492.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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