Human TFPI Antibody Summary
Accession # P10646
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Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human TFPI by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TFPI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2974) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for TFPI at approximately 40-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human TFPI by Simple WesternTM. Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for TFPI at approximately 58 kDa (as indicated) using 50 µg/mL of Goat Anti-Human TFPI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2974) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Human TFPI, also known as lipoprotein-associated coagulation inhibitor (LACI) and extrinsic pathway inhibitor (EPI), is a physiological inhibitor of extrinsic pathway of coagulation and has biological functions of anticoagulation and anti-inflammation (1). It is a secreted protein with a N-terminal acidic region, three Kunitz (K) domains separated with by two linker regions, and a C-terminal basic region (2). The first K domain (residues 54 to 104) inhibits coagulation factor VIIa complexed to tissue factor (TF). The second K domain (residues 125 to 175) inhibits factor Xa. The third K domain (residues 217 to 267) binds to heparin (3). The C-terminal basic region may have several functions. For example, it plays an important role in binding of TFPI to cell surfaces (2). The purified rhTFPI ends at residue 282 and does not contain the last 20 residues (residues 283 to 302) in the C-terminal region. It inhibits the activity of Recombinant Human Coagulation Factor VII (Catalog # 2338-SE) in the presence of Recombinant Human Coagulation Factor III/Tissue Factor (Catalog # 2339-PA).
- Bai, H. et al. (2005) Thromb Haemost. 93:1055.
- Bajaj, M.S. et al. (2001) Thromb Haemost. 86:959.
- Mine, S. et al. (2002) Biochemistry 41:78.
Citations for Human TFPI Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 4
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Aberrant stromal tissue factor localisation and mycolactone-driven vascular dysfunction, exacerbated by IL-1beta, are linked to fibrin formation in Buruli ulcer lesions
Authors: LT Hsieh, SJ Dos Santos, BS Hall, J Ogbechi, AD Loglo, FJ Salguero, MT Ruf, G Pluschke, RE Simmonds
PloS Pathogens, 2022;18(1):e1010280.
Sample Types: Cell Culture Supernates
Applications: ELISA Detection
SILAC Analysis Reveals Increased Secretion of Hemostasis-Related Factors by Senescent Cells
Authors: CD Wiley, S Liu, C Limbad, AM Zawadzka, J Beck, M Demaria, R Artwood, F Alimirah, JA Lopez-Domi, C Kuehnemann, SR Danielson, N Basisty, HG Kasler, TR Oron, PY Desprez, SD Mooney, BW Gibson, B Schilling, J Campisi, P Kapahi
Cell Rep, 2019;28(13):3329-3337.e5.
Sample Types: Cell Culture Supernates
Applications: Western Blot
Enzymatically oxidized phospholipids restore thrombin generation in coagulation factor deficiencies
Authors: DA Slatter, CL Percy, K Allen-Redp, JM Gajsiewicz, NJ Brooks, A Clayton, VJ Tyrrell, M Rosas, SN Lauder, A Watson, M Dul, Y Garcia-Dia, M Aldrovandi, M Heurich, J Hall, JH Morrissey, S Lacroix-De, S Delignat, PV Jenkins, PW Collins, VB O'Donnell
JCI Insight, 2018;3(6):.
Sample Types: Plasma
Use of an immunoaffinity-mass spectrometry-based approach for the quantification of protein biomarkers from serum samples of lung cancer patients.
Authors: Nicol GR, Han M, Kim J, Birse CE, Brand E, Nguyen A, Mesri M, FitzHugh W, Kaminker P, Moore PA, Ruben SM, He T
Mol. Cell Proteomics, 2008;7(10):1974-82.
Sample Types: Serum
Applications: Mass Spectrometry
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