Key Product Details

Species Reactivity

Human

Applications

ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 Clone # 854621R
View all TGF-beta RI/ALK-5 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TGF-beta RI/ALK-5
Leu34-Glu125
Accession # P36897

Specificity

Detects human TGF-beta RI/ALK-5 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human TGF‑ beta  RI/ALK‑5 Antibody

Application
Recommended Usage

ELISA

This antibody functions as an ELISA capture antibody when paired with Goat Anti-Human TGF‑ beta  RI/ALK‑5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3025).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Reviewed Applications

Read 1 review rated 5 using MAB3025 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TGF-beta RI/ALK-5

TGF-beta RI, also called ALK-5, is an approximately 55 kDa type I transmembrane serine/threonine receptor kinase (1, 2). It contains a cysteine-rich extracellular domain (ECD), a transmembrane helix, and a C-terminal cytoplasmic kinase domain (3). Within the cytoplasmic domain there is also a short, conserved regulatory sequence known as the GS region that is N-terminal to the kinase domain (1). Within the ECD, human TGF-beta RI shares 90% and 88% aa sequence identity with mouse and rat TGF-beta RI, respectively. In the presence of TGF-beta, TGF-beta RI forms a complex with, and is phosphorylated by, TGF-beta RII (1). Phosphorylated TGF-beta RI can then transiently bind and phosphorylate Smad2 and Smad3 (2, 4-6). These phosphorylated Smads form heteromeric complexes with Smad4, translocate to the nucleus, and regulate target gene transcription (2, 4-6). TGF-beta RI is likely important during development, since mice deficient for TGF-beta RI die at midgestation with severe defects in vascular development of the yolk sac and placenta, and an absence of circulating red blood cells (7). Furthermore, TGF-beta RI appears to be involved in proper lymphatic network development (8). Mutations in TGF-beta RI have been identified in pancreatic, colorectal, ovarian, and head and neck cancers (9).

References

  1. Wrana, J.L. et al. (1994) Nature 370:341.
  2. Massagué, J. (2012) Nat. Rev. Mol. Cell Biol. 13:616.
  3. Huse, M. et al. (1999) Cell 96:425.
  4. Marcías-Silva, M. et al. (1996) Cell 87:1215.
  5. Zhang, Y. et al. (1996) Nature 383:168.
  6. Huse, M. et al. (2001) Mol. Cell 8:671.
  7. Larsson, J. et al. (2001) EMBO J. 20:1663.
  8. James, J.M. et al. (2013) Development 140:3903.
  9. Sheen, Y.Y. et al. (2013) Biomol. Ther. (Seoul) 21:323.

Long Name

Transforming Growth Factor beta Receptor I

Alternate Names

ALK-5, SKR4, TGF-bRI, TGFbetaRI, TGFBR1

Entrez Gene IDs

7046 (Human); 21812 (Mouse); 29591 (Rat)

Gene Symbol

TGFBR1

UniProt

P36897

Additional TGF-beta RI/ALK-5 Products

Product Documents for Human TGF‑ beta  RI/ALK‑5 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human TGF‑ beta  RI/ALK‑5 Antibody

For research use only

Customer Reviews for Human TGF‑ beta  RI/ALK‑5 Antibody (1)

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  • Human TGF-beta  RI/ALK-5 Antibody
    Name: Jenna Nguyen
    Application: Affinity Purification
    Sample Tested: 4T1 mouse breast cancer cell line and 3T3-L1 mouse embryonic fibroblast adipose-like cell line
    Species: Mouse
    Verified Customer | Posted 06/17/2019

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