Human Tiki1/TRABD2A Antibody (901705)

R&D Systems | Catalog # MAB8376

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 901705
View all Tiki1/TRABD2A Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Tiki1/TRABD2A
Met1-Met477
Accession # Q86V40

Specificity

Detects human Tiki1/TRABD2A in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Tiki1/TRABD2A Antibody (901705)

Detection of Human Tiki1/TRABD2A antibody by Western Blot.

Detection of Human Tiki1/TRABD2A by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Tiki1/TRABD2A Monoclonal Antibody (Catalog # MAB8376) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Tiki1/TRABD2A at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Tiki1/TRABD2A Antibody (901705)

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Tiki1/TRABD2A

Tiki1, also known as TRAB Domain-containing protein 2A (TRABD2A) and C2orf89, is an evolutionarily conserved transmembrane metalloprotease that acts as an inhibitor of the Wnt signaling pathway. The term Tiki refers to a large-headed humanoid in Polynesian mythology, and Tiki1 was initially identified by functional screening as an organizer-specific protein that is required for head formation in Xenopus. Tiki1 negatively regulates Wnt signaling by mediating the cleavage of the eight N-terminal residues from a subset of Wnt proteins, including Wnt3A and Wnt5, but not Wnt11. Following this cleavage, Wnt proteins become oxidized and form large disulfide-bond oligomers, leading to their inactivation. Human Tiki1 is 505 amino acids (aa) in length; a second shorter isoform has also been identified that is missing aa 225-273.

Alternate Names

C2orf89, LOC129293, TRABD2A

Entrez Gene IDs

129293 (Human)

Gene Symbol

TRABD2A

UniProt

Q86V40

Additional Tiki1/TRABD2A Products

Product Documents for Human Tiki1/TRABD2A Antibody (901705)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Tiki1/TRABD2A Antibody (901705)

For research use only

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