Human Tiki1/TRABD2A Antibody

Catalog #: MAB8376 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB8376
MAB8376-SP

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Detection of Human Tiki1/TRABD2A by Western Blot.

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Human Tiki1/TRABD2A Antibody Summary

Species Reactivity

Human

Specificity

Detects human Tiki1/TRABD2A in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG_2A Clone # 901705

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Tiki1/TRABD2A
Met1-Met477
Accession # Q86V40

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Tiki1/TRABD2A by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Tiki1/TRABD2A Monoclonal Antibody (Catalog # MAB8376) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Tiki1/TRABD2A at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Tiki1/TRABD2A

Tiki1, also known as TRAB Domain-containing protein 2A (TRABD2A) and C2orf89, is an evolutionarily conserved transmembrane metalloprotease that acts as an inhibitor of the Wnt signaling pathway. The term Tiki refers to a large-headed humanoid in Polynesian mythology, and Tiki1 was initially identified by functional screening as an organizer-specific protein that is required for head formation in Xenopus. Tiki1 negatively regulates Wnt signaling by mediating the cleavage of the eight N-terminal residues from a subset of Wnt proteins, including Wnt3A and Wnt5, but not Wnt11. Following this cleavage, Wnt proteins become oxidized and form large disulfide-bond oligomers, leading to their inactivation. Human Tiki1 is 505 amino acids (aa) in length; a second shorter isoform has also been identified that is missing aa 225-273.

Entrez Gene IDs

129293 (Human)

Alternate Names

C2orf89; C2orf89;TIKI1;TRABD2A; LOC129293; Tiki1; TRABD2A

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

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