Human TIMP-2 Antibody
Human TIMP-2 Antibody Summary
Cys27-Pro220
Accession # P16035
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
Detection of Human TIMP‑2 by Western Blot. Western blot shows Recombinant Human TIMP-2 Western Blot Standard Protein (Catalog # WBC023) and lysates of HeLa human cervical epithelial carcinoma cell line and human placenta tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human TIMP-2 Monoclonal Antibody (Catalog # MAB971) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for TIMP-2 at approximately 22 kDa (as indicated).This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
View Larger
TIMP‑2 in Human Ovarian Cancer Tissue. TIMP-2 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using 15 µg/mL Mouse Anti-Human TIMP-2 Monoclonal Antibody (Catalog # MAB971) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS003) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TIMP-2
Tissue inhibitors of metalloproteinases or TIMPs are a family of proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs). There are four members of the family, TIMP-1, TIMP-2, TIMP-3, and TIMP-4. TIMP-2 is a non N-glycosylated protein with a molecular mass of 22 kDa produced by a wide range of cell types, which inhibits MMPs non-covalently by the formation of binary complexes. TIMP-2 also has erythroid-potentiating and cell growth promoting activities.
Product Datasheets
Citations for Human TIMP-2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
8
Citations: Showing 1 - 8
Filter your results:
Filter by:
-
MicroRNA?122 acts as tumor suppressor by targeting TRIM29 and blocking the activity of PI3K/AKT signaling in nasopharyngeal carcinoma in�vitro
Authors: Y Yang, Q Li, L Guo
Mol Med Rep, 2018;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Granule exocytosis is required for platelet spreading: differential sorting of alpha-granules expressing VAMP-7.
Blood, 2012;120(1):199-206.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Conditionally replicating adenovirus expressing TIMP2 for ovarian cancer therapy.
Authors: Yang SW, Cody JJ, Rivera AA, Waehler R, Wang M, Kimball KJ, Alvarez RA, Siegal GP, Douglas JT, Ponnazhagan S
Clin. Cancer Res., 2011;17(3):538-49.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
The DSCs-expressed CD82 controls the invasiveness of trophoblast cells via integrinbeta1/MAPK/MAPK3/1 signaling pathway in human first-trimester pregnancy.
Authors: Li MQ, Hou XF, Shao J, Tang CL, Li DJ
Biol. Reprod., 2010;82(5):968-79.
Species: Human
Sample Types: Whole Cells
Applications: Cell-based ELISA -
Tissue inhibitors of matrix metalloproteinases in platelets and megakaryocytes: a novel organization for these secreted proteins.
Authors: Villeneuve J, Block A, Le Bousse-Kerdiles MC, Lepreux S, Nurden P, Ripoche J, Nurden AT
Exp. Hematol., 2009;37(7):849-56.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Matrix metalloproteinase activity in pediatric acute lung injury.
Authors: Kong MY, Gaggar A, Li Y
Int J Med Sci, 2009;6(1):9-17.
Species: Human
Sample Types: Tissue Secretion
Applications: Western Blot -
IL-21 is highly produced in Helicobacter pylori-infected gastric mucosa and promotes gelatinases synthesis.
Authors: Caruso R, Fina D, Peluso I, Fantini MC, Tosti C, Del Vecchio Blanco G, Paoluzi OA, Caprioli F, Andrei F, Stolfi C, Romano M, Ricci V, MacDonald TT, Pallone F, Monteleone G
J. Immunol., 2007;178(9):5957-65.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot -
Control of matrix metalloproteinase production in human intestinal fibroblasts by interleukin 21.
Authors: Monteleone G, Caruso R, Fina D, Peluso I, Gioia V, Stolfi C, Fantini MC, Caprioli F, Tersigni R, Alessandroni L, MacDonald TT, Pallone F
Gut, 2006;55(12):1774-80.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsIsotype Controls
Reconstitution Buffers
Secondary Antibodies
Reviews for Human TIMP-2 Antibody
Average Rating: 5 (Based on 1 Review)
Have you used Human TIMP-2 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by:
