Human TLR2 Antibody
Human TLR2 Antibody Summary
Glu21-Leu590
Accession # O60603
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Applications
Human TLR2 Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human TLR2 by Western Blot. Western blot shows lysate of human lung tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TLR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2616) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TLR2 at approximately 90-100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TLR2
Human toll-like receptor (TLR) family includes ten members that activate the innate immune response via an ability to recognize molecular structures found in a variety of microbial pathogens (1‑3). All TLR family members are type I transmembrane proteins with a large number of extracellular leucine-rich repeats (LRRs) and a cytoplasmic Toll/IL-1 receptor (TIR) domain. Human TLR2 is synthesized as a 784 amino acid (aa) precursor (2) that contains a signal sequence (aa 1-18), an extracellular domain (aa 19-588) with approximately 20 LRRs, a transmembrane segment (aa 589-609), and a cytoplasmic TIR domain (aa 610-784). The receptor is expressed on a number of cell types including monocytes, dendritic cells, neutrophils, B cells endothelial cells, and hepatocytes (1, 2, 4). TLR2 functions as part of a heterodimeric complex with either TLR1 or TLR6, and possibly other co-receptors (1). These complexes recognize lipoproteins and glycolipids from gram-positive and gram-negative bacteria as well as mycoplasma and yeast. TLR2/TLR1 heterodimers bind triacylated lipopeptides, while the TLR2/TLR6 heterodimer preferentially recognizes diacylated lipopeptides (5). Upon ligand recognition, TLR2 delivers an activating signal via the associated adapter molecules, MyD88 and TIRAP (1, 6). TLR2 signaling results in dendritic cell maturation characterized by increased surface expression of class II MHC and the T cell costimulators, CD80 and CD86 (1, 2). Activation via TLR2 also results in production of a number of pro-inflammatory cytokines including TNF-alpha, IL-2, IL-6, IL-12, and MIP-2 (1-3).
- Wetzler, L. (2003) Vaccine 21:S2/55.
- Kirschning, C. and R. Schumann (2002) Curr. Top. microbiol. Immunol. 270:121.
- Netea, M. et al. (2004) J. Leukoc. Biol. 75:749.
- Flo T. et al. (2001) J. Leukoc. Biol. 69:474.
- Akira S. (2003) Curr. Opin. Immunol. 15:5.
- Yamamoto M. et al. (2002) Nature 420:324.
Product Datasheets
Citations for Human TLR2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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LYSMD3: A mammalian pattern recognition receptor for chitin
Authors: X He, BA Howard, Y Liu, AK Neumann, L Li, N Menon, T Roach, SD Kale, DC Samuels, H Li, T Kite, H Kita, TY Hu, M Luo, CN Jones, UJ Okaa, DL Squillace, BS Klein, CB Lawrence
Cell Reports, 2021;36(3):109392.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Soluble Siglec-14 glycan-recognition protein is generated by alternative splicing and suppresses myeloid inflammatory responses
Authors: PJ Huang, PY Low, I Wang, SD Hsu, T Angata
J. Biol. Chem., 2018;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation -
Toll-like receptor 2 is increased in neurons in Parkinson's disease brain and may contribute to alpha-synuclein pathology
Acta Neuropathol., 2016;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
ERK5 protein promotes, whereas MEK1 protein differentially regulates, the Toll-like receptor 2 protein-dependent activation of human endothelial cells and monocytes.
Authors: Wilhelmsen K, Mesa K, Lucero J, Xu F, Hellman J
J Biol Chem, 2012;287(32):26478-94.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Maternal high-fat diet disturbs uteroplacental hemodynamics and increases the frequency of stillbirth in a nonhuman primate model of excess nutrition.
Authors: Frias AE, Morgan TK, Evans AE
Endocrinology, 2011;152(6):2456-64.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Bacterial lipoprotein TLR2 agonists broadly modulate endothelial function and coagulation pathways in vitro and in vivo.
Authors: Shin HS, Xu F, Bagchi A, Herrup E, Wilhelmsen K
J. Immunol., 2011;186(2):1119-30.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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