TMEM59 in HeLa Human Cell Line. TMEM59 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human TMEM59 Monoclonal Antibody (Catalog # MAB9118) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
TMEM59 is a
324 aa protein with a 21 amino acid transmembrane domain which localizes to the
Golgi compartment where it modulates the O-glycosylation and complex
N-glycosylation maturation steps of several proteins including APP, BACE1, SEAP
and PRNP. It may retain APP in the Golgi
and inhibits amyloid beta generation as well APP cleavage by alpha and beta
secretases. TMEM59 also contains a 19 aa peptide that acts as a regulator of
autophagy in response to bacterial infection by promoting LC3 activation
through interaction with ATG16L1, targeting its own endosomal compartment to
lysosomes in response to aggregation during S.areus infection.
Ullrich S, et al, J Biol Chem. 2010
Boada-Romero E, et al, EMBO J. 2013
Transmembrane Protein 59
Entrez Gene IDs:
9528 (Human); 56374 (Mouse); 100196907 (Rat)
C1orf8; chromosome 1 open reading frame 8; DCF1; FLJ42977; HSPC001; Liver membrane-bound protein; PRO195; TMEM59; transmembrane protein 59; UNQ169
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