Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 816004
View all TMEM59 Primary Antibodies »

Product Specifications

Immunogen

E.coli-derived recombinant human TMEM59
Met1-Asn220
Accession # Q9BXS4

Specificity

Detects human TMEM59 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human TMEM59 Antibody

TMEM59 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

TMEM59 in HeLa Human Cell Line.

TMEM59 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human TMEM59 Monoclonal Antibody (Catalog # MAB9118) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human TMEM59 Antibody

Application
Recommended Usage

Immunocytochemistry

5-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TMEM59

TMEM59 is a 324 aa protein with a 21 amino acid transmembrane domain which localizes to the Golgi compartment where it modulates the O-glycosylation and complex N-glycosylation maturation steps of several proteins including APP, BACE1, SEAP and PRNP. It may retain APP in the Golgi and inhibits amyloid beta generation as well APP cleavage by alpha and beta secretases. TMEM59 also contains a 19 aa peptide that acts as a regulator of autophagy in response to bacterial infection by promoting LC3 activation through interaction with ATG16L1, targeting its own endosomal compartment to lysosomes in response to aggregation during S.areus infection.

References

  1. Ullrich S, et al, J Biol Chem. 2010 Jul 2;285(27):20664
  2. Boada-Romero E, et al, EMBO J. 2013 Feb 20;32(4):566

Long Name

Transmembrane Protein 59

Alternate Names

C1orf8, DCF1, HSPC001, PRO195, UNQ169

Entrez Gene IDs

9528 (Human); 56374 (Mouse); 100196907 (Rat)

Gene Symbol

TMEM59

UniProt

Q9BXS4

Additional TMEM59 Products

Product Documents for Human TMEM59 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human TMEM59 Antibody

For research use only

Related Research Areas

Autophagy

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