Human TMEM59 Antibody

Catalog # Availability Size / Price Qty
MAB9118
MAB9118-SP
TMEM59  in HeLa Human Cell Line.
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Human TMEM59 Antibody Summary

Species Reactivity
Human
Specificity
Detects human TMEM59 in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 816004
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E.coli-derived recombinant human TMEM59
Met1-Asn220
Accession # Q9BXS4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunocytochemistry
5-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry TMEM59  in HeLa Human Cell Line. View Larger

TMEM59 in HeLa Human Cell Line. TMEM59 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human TMEM59 Monoclonal Antibody (Catalog # MAB9118) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TMEM59

TMEM59 is a 324 aa protein with a 21 amino acid transmembrane domain which localizes to the Golgi compartment where it modulates the O-glycosylation and complex N-glycosylation maturation steps of several proteins including APP, BACE1, SEAP and PRNP. It may retain APP in the Golgi and inhibits amyloid beta generation as well APP cleavage by alpha and beta secretases. TMEM59 also contains a 19 aa peptide that acts as a regulator of autophagy in response to bacterial infection by promoting LC3 activation through interaction with ATG16L1, targeting its own endosomal compartment to lysosomes in response to aggregation during S.areus infection.

References
  1. Ullrich S, et al, J Biol Chem. 2010 Jul 2;285(27):20664
  2. Boada-Romero E, et al, EMBO J. 2013 Feb 20;32(4):566
Long Name
Transmembrane Protein 59
Entrez Gene IDs
9528 (Human); 56374 (Mouse); 100196907 (Rat)
Alternate Names
C1orf8; chromosome 1 open reading frame 8; DCF1; FLJ42977; HSPC001; Liver membrane-bound protein; PRO195; TMEM59; transmembrane protein 59; UNQ169

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Cell and Tissue Staining Kits

Isotype Controls

Mouse IgG1 Isotype Control

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Reconstitution Buffers

Secondary Antibodies

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