Human TMPRSS2 Antibody Summary
Accession # O15393
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Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of TMPRSS2 in HEK293 Human Cell Line Transfected with Human TMPRSS2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human TMPRSS2 or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human TMPRSS2 Monoclonal Antibody (Catalog # MAB107231) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on control antibody staining (MAB0041). Staining was performed using our Staining Membrane-associated Proteins protocol.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
TMPRSS2 belongs to the serine protease family. It contains a type II transmembrane domain, a receptor class A domain, a scavenger receptor cysteine-rich domain and a protease domain. Serine proteases are known to be involved in many physiological and pathological processes. TMPRSS2 facilitates human SARS coronavirus (SARS-CoV) infection via two independent mechanisms: proteolytic cleavage of ACE2, which might promote viral uptake, and cleavage of coronavirus spike glycoprotein, which activates the glycoprotein for cathepsin Lindependent host cell entry. Alternatively spliced transcripts encoding different proteins have been described.
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