Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1042528
View all TMPRSS9 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human TMPRSS9
Glu190-Gln490
Accession # Q7Z410

Specificity

Detects human TMPRSS9 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human TMPRSS9 MAb (Clone 1042528)

TMPRSS9 in SARS-CoV-2 infected Human lung.

TMPRSS9 was detected in immersion fixed paraffin-embedded sections of SARS-CoV-2 infected human lung using Mouse Anti-Human TMPRSS9 Monoclonal Antibody (Catalog # MAB5688) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to immunoreactive profiles scattered throughout the tissue. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human TMPRSS9 MAb (Clone 1042528)

Application
Recommended Usage

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of SARS-CoV-2 infected human lung

Reviewed Applications

Read 1 review rated 5 using MAB5688 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TMPRSS9

TMPRSS9 (Transmembrane protease, Serine 9; also Polyserine protease 1) is a 116-120 kDa (predicted) member of the TTSP family of membrane-bound serine proteases. It is expressed in fetal tissue, and converts pro-uPA into its active form. Human TMPRSS9 is a 1059 type II transmembrane (TM) protein. It has a 29 aa N-terminal cytoplasmic region and a 1008 aa extracellular domain (ECD) (aa 51-1059). The ECD contains one LDLR region (aa 153-190), followed by three Peptidase S1 domains (aa 203-436, 504-736, and 827-1058), the first two of which are catalytically active. Proteolytic cleavage can generate a soluble 34 kDa Serase 1 (aa 203-503), a 35 kDa Serase 2 (aa 504-826) and an inactive 25 kDa Serase 3 (aa 827-1059). Alternatively, truncated, TM forms of TMPRSS9 such as a 56 kDa TM isotype containing only the Serase 1 domain,  or a 91 kDa (predicted) form containing the Serase 1, 2 and 3 domains may exist. Two potential splice forms are reported. One shows an insertion of 34 aa after Lys90 accompanied by a seven aa substitution for aa 491-1059. A second isoform shows a 34 aa substitution for aa 822-1059. Over aa 190-490, human TMPRSS9 shares 79% aa identity with mouse TMPRSS9.

Long Name

Transmembrane protease, serine 9

Alternate Names

Polyserase-1, Polyserase-I

Entrez Gene IDs

360200 (Human); 432478 (Mouse); 314636 (Rat)

Gene Symbol

TMPRSS9

UniProt

Q7Z410

Additional TMPRSS9 Products

Product Documents for Human TMPRSS9 MAb (Clone 1042528)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human TMPRSS9 MAb (Clone 1042528)

For research use only

Related Research Areas

Serine Proteases and Regulators

Customer Reviews for Human TMPRSS9 MAb (Clone 1042528) (1)

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  • Human TMPRSS9 MAb (Clone 1042528)
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Lung tissue
    Species: Human
    Verified Customer | Posted 10/23/2022
    Bronchial tissue
    Human TMPRSS9 MAb (Clone 1042528) MAB5688

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Protocols

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