Human TNF-alpha Quantikine HS ELISA

HSTA00D has been discontinued and is replaced by HSTA00E.
  (95 citations)
(3 Reviews)
    
Datasheet / CoA / SDS
Product Details
Assay Procedure
Citations (95)
FAQs
Supplemental Products
Reviews
  • Assay Length
    6.5 hours
  • Sample Type & Volume Required Per Well
    Serum (200 uL), EDTA Plasma (200 uL), Heparin Plasma (200 uL), Citrate Plasma (200 uL)
  • Sensitivity
    0.191 pg/mL
  • Assay Range
    0.5 - 32 pg/mL (Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
  • Specificity
    Natural and recombinant human TNF-alpha
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Product Summary
The Quantikine HS Human TNF-alpha Immunoassay is a 6.5 hour solid phase ELISA designed to measure TNF-alpha in serum and plasma. It contains E. coli-derived recombinant human TNF-alpha and antibodies raised against the recombinant factor. It has been shown to accurately quantitate recombinant human TNF-alpha. Results obtained with naturally occurring TNF-alpha samples showed linear curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural TNF-alpha. Since the measurement of TNF-alpha is insensitive to the addition of recombinant forms of either of the two types of soluble receptors, it is probable that this measurement detects the total amount of TNF-alpha in samples, i.e., the total amount of free TNF-alpha plus the amount of TNF-alpha bound to soluble receptors.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components
Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 41 41 41
Mean 1.96 11.5 22.1 1.83 10.5 20.3
Standard Deviation 0.17 0.49 0.68 0.19 0.76 1.5
CV% 8.7 4.3 3.1 10.4 7.2 7.4

Recovery

The recovery of TNF-alpha was determined by spiking to three different levels throughout the range of the assay in various matrices.

Sample Type Average % Recovery Range %
Citrate Plasma (n=4) 95 88-105
EDTA Plasma (n=4) 99 92-111
Heparin Plasma (n=4) 93 87-103
Serum (n=4) 93 85-98
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of TNF-alpha and diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
 TNF-alpha [HRP]
Product Datasheets

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Certificate of Analysis Lookup

Certificate of Analysis Request Form

To download a Certificate of Analysis, please enter the catalog and lot numbers in the search box below.

Note: Certificate of Analysis not available for kit components.

Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: TNF-alpha

Tumor necrosis factor alpha (TNF-α), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, apoptosis, and immune system development. TNF-α is produced by a wide variety of immune and epithelial cell types. Human TNF-α consists of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 177 aa extracellular domain (ECD). Within the ECD, human TNF-α shares 97% aa sequence identity with rhesus and 71% - 92% with bovine, canine, cotton rat, equine, feline, mouse, porcine, and rat TNF-α. The 26 kDa type 2 transmembrane protein is assembled intracellularly to form a noncovalently linked homotrimer. Ligation of this complex induces reverse signaling that promotes lymphocyte costimulation but diminishes monocyte responsiveness.

Cleavage of membrane bound TNF-α by TACE/ADAM17 releases a 55 kDa soluble trimeric form of TNF-α. TNF-α trimers bind the ubiquitous TNF RI and the hematopoietic cell-restricted TNF RII, both of which are also expressed as homotrimers. TNF-α regulates lymphoid tissue development through control of apoptosis. It also promotes inflammatory responses by inducing the activation of vascular endothelial cells and macrophages. TNF-α is a key cytokine in the development of several inflammatory disorders. It contributes to the development of type 2 diabetes through its effects on insulin resistance and fatty acid metabolism.

  • Long Name:
    Tumor Necrosis Factor alpha
  • Entrez Gene IDs:
    7124 (Human); 21926 (Mouse); 24835 (Rat); 397086 (Porcine); 280943 (Bovine); 403922 (Canine); 100033834 (Equine); 493755 (Feline); 100009088 (Rabbit)
  • Alternate Names:
    APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; tnfa; tnf-a; TNFalpha; TNF-alpha; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor-alpha
Related Research Areas
Assay Procedure
Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 50 µL Assay Diluent
  4.   Add 50 µL of Assay Diluent to each well.

  5. 200 µL Standard, Control, or Sample
  6.   Add 200 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 3 hours.
  7.   Aspirate each well and wash, repeating the process 5 times for a total of 6 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  10.   Aspirate and wash 6 times.

  11. 50 µL Substrate Solution
  12.   Add 50 µL Substrate Solution to each well. Cover with a new plate sealer, and incubate at room temperature for 1 hour. Do not wash the plate.

  13. 50 µL Amplifier Solution
  14.   Add 50 µL Amplifier Solution to each well. Cover with a new plate sealer, and incubate at room temperature for 30 minutes.

  15. 50 µL Stop Solution
  16. Add 50 µL of Stop Solution to each well. Read at 490 nm within 30 minutes. Set wavelength correction to 650 nm or 690 nm.
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

95 Citations: Showing 1 - 10
Filter your results:

Species
Sample Type
  1. Effects of the Ketogenic diet in overweight divers breathing Enriched Air Nitrox
    Authors: G Bosco, A Rizzato, S Quartesan, E Camporesi, D Mangar, M Paganini, L Cenci, S Malacrida, S Mrakic-Spo, S Moretti, A Paoli
    Sci Rep, 2018;8(1):2655.
    Species: Human
    Sample Type: Plasma
  2. Intracellular localization of diacylglycerols and sphingolipids influences insulin sensitivity and mitochondrial function in human skeletal muscle
    Authors: L Perreault, SA Newsom, A Strauss, A Kerege, DE Kahn, KA Harrison, JK Snell-Berg, T Nemkov, A D'Alessand, MR Jackman, PS MacLean, BC Bergman
    JCI Insight, 2018;3(3):.
    Species: Human
    Sample Type: Plasma
  3. Contribution of bacterial pathogens to evoking serological disease markers and aggravating disease activity in rheumatoid arthritis
    Authors: K Terato, T Waritani, R Fukai, H Shionoya, H Itoh, K Katayama
    PLoS ONE, 2018;13(2):e0190588.
    Species: Human
    Sample Type: Serum
  4. Ghrelin and hormonal markers under exercise training in patients with heart failure with preserved ejection fraction: results from the Ex-DHF pilot study
    Authors: TD Trippel, V Holzendorf, M Halle, G Gelbrich, K Nolte, A Duvinage, S Schwarz, T Rutscher, J Wiora, R Wachter, C Herrmann-L, HD Duengen, G Hasenfu beta, B Pieske, F Edelmann
    ESC Heart Fail, 2017;4(1):56-65.
    Species: Human
    Sample Type: Serum
  5. Repeated Excessive Exercise Attenuates the Anti-Inflammatory Effects of Exercise in Older Men
    Authors: RE Sahl, PR Andersen, K Gronbaek, TH Morville, M Rosenkilde, HK Rasmusen, SS Poulsen, C Prats, F Dela, JW Helge
    Front Physiol, 2017;8(0):407.
    Species: Human
    Sample Type: Plasma
  6. Heat and Hypoxic Acclimation Increase Monocyte Heat Shock Protein 72 but Do Not Attenuate Inflammation following Hypoxic Exercise
    Authors: BJ Lee, CD Thake
    Front Physiol, 2017;8(0):811.
    Species: Human
    Sample Type: Plasma
  7. Microenvironment inflammatory infiltrate drives growth speed and outcome of hepatocellular carcinoma: a prospective clinical study
    Authors: R Critelli, F Milosa, F Faillaci, R Condello, E Turola, L Marzi, B Lei, F Dituri, S Andreani, P Sighinolfi, P Manni, A Maiorana, C Caporali, F di Benedet, M Del Buono, N De Maria, F Schepis, ML Martinez-C, G Giannelli, E Villa
    Cell Death Dis, 2017;8(8):e3017.
    Species: Human
    Sample Type: Serum
  8. Sarcopenia correlates with systemic inflammation in COPD
    Authors: MK Byun, EN Cho, J Chang, CM Ahn, HJ Kim
    Int J Chron Obstruct Pulmon Dis, 2017;12(0):669-675.
    Species: Human
    Sample Type:
  9. Chemokine signals are crucial for enhanced homing and differentiation of circulating osteoclast progenitor cells
    Authors: A Sucur, Z Jajic, M Artukovic, MI Matijasevi, B Anic, D Flegar, A Markotic, T Kelava, S Ivcevic, N Kovacic, V Katavic, D Grcevic
    Arthritis Res. Ther., 2017;19(1):142.
    Species: Human
    Sample Type: Plasma
  10. The association between three major physiological stress systems and oxidative DNA and lipid damage
    Authors: CN Black, M Bot, D Révész, PG Scheffer, B Penninx
    Psychoneuroendocrinology, 2017;80(0):56-66.
    Species: Human
    Sample Type: Plasma
  11. Immunometabolic dysregulation is associated with reduced cortical thickness of the anterior cingulate cortex
    Authors: Laura S van Velzen
    Brain Behav. Immun, 2017;60(0):361-368.
    Species: Human
    Sample Type: Plasma
  12. Visceral fat area is a strong predictor of leukocyte cell-derived chemotaxin 2, a potential biomarker of dyslipidemia
    Authors: K Tanisawa, H Taniguchi, X Sun, T Ito, R Kawakami, S Sakamoto, M Higuchi
    PLoS ONE, 2017;12(3):e0173310.
    Species: Human
    Sample Type: Serum
  13. Tryptophan Metabolism in Patients With Chronic Kidney Disease Secondary to Type 2 Diabetes: Relationship to Inflammatory Markers
    Authors: S Debnath, C Velagapudi, L Redus, F Thameem, B Kasinath, CE Hura, C Lorenzo, HE Abboud, JC O'Connor
    Int J Tryptophan Res, 2017;10(0):1178646917694.
    Species: Human
    Sample Type: Plasma
  14. Inflammation and Arterial Stiffness in Chronic Kidney Disease: Findings From the CRIC Study
    Authors: E Peyster, J Chen, HI Feldman, AS Go, J Gupta, N Mitra, Q Pan, A Porter, M Rahman, D Raj, M Reilly, MR Wing, W Yang, RR Townsend,
    Am. J. Hypertens., 2017;30(4):400-408.
    Species: Human
    Sample Type: Plasma
  15. Association of plasma CD40L with acute chest syndrome in sickle cell anemia
    Authors: VT Garrido, L Sonzogni, SN Mtatiro, FF Costa, N Conran, SL Thein
    Cytokine, 2017;97(0):104-107.
    Species: Human
    Sample Type: Plasma
  16. Reduction of c-Fos via Overexpression of miR-34a Results in Enhancement of TNF- Production by LPS in Neutrophils from Myelodysplastic Syndrome Patients
    PLoS ONE, 2016;11(8):e0158527.
    Species: Human
    Sample Type: Cell Culture Supernates
  17. Trait Hostility and Acute Inflammatory Responses to Stress in the Laboratory
    Authors: Dominique Girard
    PLoS ONE, 2016;11(6):e0156329.
    Species: Human
    Sample Type: Serum
  18. Comparative analysis of three human adipocyte size measurement methods and their relevance for cardiometabolic risk
    Authors: André Tchernof
    Obesity (Silver Spring), 2016;0(0):.
    Species: Human
    Sample Type: Plasma
  19. Circadian misalignment increases cardiovascular disease risk factors in humans
    Proc. Natl. Acad. Sci. U.S.A., 2016;113(10):E1402-11.
    Species: Human
    Sample Type: Serum
  20. Local TNF causes NFATc1-dependent cholesterol-mediated podocyte injury
    J Clin Invest, 2016;0(0):.
    Species: Human
    Sample Type: Serum
  21. Effects of nateglinide and rosiglitazone on pancreatic alpha- and beta-cells, GLP-1 secretion and inflammatory markers in patients with type 2 diabetes: randomized crossover clinical study.
    Authors: Tostes G, Cunha M, Fukui R, Correia M, Rocha D, Dos Santos R, da Silva M
    Diabetol Metab Syndr, 2016;8(0):1.
    Species: Human
    Sample Type: Serum
  22. Interleukin-6 Is a Risk Factor for Atrial Fibrillation in Chronic Kidney Disease: Findings from the CRIC Study.
    Authors: Amdur R, Mukherjee M, Go A, Barrows I, Ramezani A, Shoji J, Reilly M, Gnanaraj J, Deo R, Roas S, Keane M, Master S, Teal V, Soliman E, Yang P, Feldman H, Kusek J, Tracy C, Raj D
    PLoS ONE, 2016;11(2):e0148189.
    Species: Human
    Sample Type: Plasma
  23. Casein glycomacropeptide for active distal ulcerative colitis: a randomised pilot study
    Authors: CL Hvas, A Dige, M Bendix, PG Wernlund, LA Christense, JF Dahlerup, J Agnholt
    Eur J Clin Invest, 2016;0(0):.
    Species: Human
    Sample Type: Plasma
  24. The relationship between circulating neutrophil gelatinase-associated lipocalin and early alteration of metabolic parameters is associated with dietary saturated fat intake in non-diabetic Korean women
    Authors: GY Na, SR Yoon, J An, R Yeo, J Song, MN Jo, S Han, OY Kim
    Endocr. J, 2016;0(0):.
    Species: Human
    Sample Type: Plasma
  25. Anti-TNF-? Drugs Differently Affect the TNF?-sTNFR System and Monocyte Subsets in Patients with Psoriasis
    PLoS ONE, 2016;11(12):e0167757.
  26. Nitric oxide and tumour necrosis factor alpha in the process of pseudoexfoliation glaucoma
    Authors: TS Sarenac Vu, SM Pavlovic, VLj Jakovljevi, KB Janicijevi, NS Zdravkovic
    Int J Ophthalmol, 2016;9(8):1138-42.
    Species: Human
    Sample Type: Serum
  27. Inflammatory and immunological profiles in patients with COPD: relationship with FEV 1 reversibility
    Authors: Cleriston Farias Queiroz
    J Bras Pneumol, 2016;42(4):241-247.
    Species: Human
    Sample Type: Serum
  28. Association study of arcuate nucleus neuropeptide Y neuron receptor gene variation and serum NPY levels in clozapine treated patients with schizophrenia
    Authors: E Leinonen
    Eur. Psychiatry, 2016;40(0):13-19.
    Species: Human
    Sample Type: Serum
  29. Tumor-specific cytotoxic T lymphocyte activity determines colorectal cancer patient prognosis.
    Authors: Reissfelder C, Stamova S, Gossmann C, Braun M, Bonertz A, Walliczek U, Grimm M, Rahbari N, Koch M, Saadati M, Benner A, Buchler M, Jager D, Halama N, Khazaie K, Weitz J, Beckhove P
    J Clin Invest, 2015;125(2):739-51.
    Species: Human
    Sample Type: Tissue Homogenates
  30. Association between Inflammation and Cardiac Geometry in Chronic Kidney Disease: Findings from the CRIC Study.
    Authors: Gupta J, Dominic E, Fink J, Ojo A, Barrows I, Reilly M, Townsend R, Joffe M, Rosas S, Wolman M, Patel S, Keane M, Feldman H, Kusek J, Raj D
    PLoS ONE, 2015;10(4):e0124772.
    Species: Human
    Sample Type: Plasma
  31. A randomized, placebo controlled trial of omega-3 fatty acids in the treatment of young children with autism.
    Authors: Mankad D, Dupuis A, Smile S, Roberts W, Brian J, Lui T, Genore L, Zaghloul D, Iaboni A, Marcon P, Anagnostou E
    Mol Autism, 2015;6(0):18.
    Species: Human
    Sample Type: Plasma
  32. Sclerostin, TNF-alpha and Interleukin-18 Correlate and are Together with Klotho Related to Other Growth Factors and Cytokines in Haemodialysis Patients.
    Authors: Almroth G, Lonn J, Uhlin F, Brudin L, Andersson B, Hahn-Zoric M
    Scand J Immunol, 2015;83(1):58-63.
    Species: Human
    Sample Type: Serum
  33. Effects of pentoxifylline on proteinuria and glucose control in patients with type 2 diabetes: a prospective randomized double-blind multicenter study.
    Authors: Han S, Kim H, Kim D, Sheen S, Chung C, Ahn C, Kim S, Cho Y, Park S, Kim S, Kim C, Kim K, Lee K
    Diabetol Metab Syndr, 2015;7(0):64.
    Species: Human
    Sample Type: Serum
  34. Tumor necrosis factor-alpha confers cardioprotection through ectopic expression of keratins K8 and K18.
    Authors: Papathanasiou S, Rickelt S, Soriano M, Schips T, Maier H, Davos C, Varela A, Kaklamanis L, Mann D, Capetanaki Y
    Nat Med, 2015;21(9):1076-84.
    Species: Human
    Sample Type: Serum
  35. Disturbances in Hypothalamic-Pituitary-Adrenal Axis and Immunological Activity Differentiating between Unipolar and Bipolar Depressive Episodes.
    Authors: Becking K, Spijker A, Hoencamp E, Penninx B, Schoevers R, Boschloo L
    PLoS ONE, 2015;10(7):e0133898.
    Species: Human
    Sample Type: Plasma
  36. Association of Sarcopenic Obesity with Higher Serum High-Sensitivity C-Reactive Protein Levels in Chinese Older Males--A Community-Based Study (Taichung Community Health Study-Elderly, TCHS-E).
    Authors: Yang C, Li C, Li T, Liu C, Lin C, Lin W, Lin C
    PLoS ONE, 2015;10(7):e0132908.
    Species: Human
    Sample Type: Serum
  37. Effects of Acute Endurance Exercise Performed in the Morning and Evening on Inflammatory Cytokine and Metabolic Hormone Responses.
    Authors: Kim H, Konishi M, Takahashi M, Tabata H, Endo N, Numao S, Lee S, Kim Y, Suzuki K, Sakamoto S
    PLoS ONE, 2015;10(9):e0137567.
    Species: Human
    Sample Type: Plasma
  38. Plasma TNF-alpha and Soluble TNF Receptor Levels after Doxorubicin with or without Co-Administration of Mesna-A Randomized, Cross-Over Clinical Study.
    Authors: Hayslip J, Dressler E, Weiss H, Taylor T, Chambers M, Noel T, Miriyala S, Keeney J, Ren X, Sultana R, Vore M, Butterfield D, St Clair D, Moscow J
    PLoS ONE, 2015;10(4):e0124988.
    Species: Human
    Sample Type: Plasma
  39. Differential Patterns and Determinants of Cardiac Autonomic Nerve Dysfunction during Endotoxemia and Oral Fat Load in Humans.
    Authors: Ziegler D, Strom A, Strassburger K, Nowotny B, Zahiragic L, Nowotny P, Carstensen-Kirberg M, Herder C, Szendroedi J, Roden M
    PLoS ONE, 2015;10(4):e0124242.
    Species: Human
    Sample Type: Serum
  40. The protective effect of esculentoside a on experimental acute liver injury in mice.
    Authors: Zhang F, Wang X, Qiu X, Wang J, Fang H, Wang Z, Sun Y, Xia Z
    PLoS ONE, 2014;9(11):e113107.
    Species: Human
    Sample Type: Cell Culture Supernates
  41. Compartment differences of inflammatory activity in chronic obstructive pulmonary disease.
    Authors: Ji J, von Scheele I, Bergstrom J, Billing B, Dahlen B, Lantz A, Larsson K, Palmberg L
    Respir Res, 2014;15(0):104.
    Species: Human
    Sample Type: Serum
  42. Big breakfast rich in protein and fat improves glycemic control in type 2 diabetics.
    Authors: Rabinovitz H, Boaz M, Ganz T, Jakubowicz D, Matas Z, Madar Z, Wainstein J
    Obesity (Silver Spring), 2014;22(5):E46-54.
    Species: Human
    Sample Type: Plasma
  43. Smac mimetics and innate immune stimuli synergize to promote tumor death.
    Authors: Beug S, Tang V, Lacasse E, Cheung H, Beauregard C, Brun J, Nuyens J, Earl N, St-Jean M, Holbrook J, Dastidar H, Mahoney D, Ilkow C, Le Boeuf F, Bell J, Korneluk R
    Nat Biotechnol, 2014;32(2):182-90.
    Species: Human
    Sample Type: Cell Culture Supernates
  44. Race modifies the association between adiposity and inflammation in patients with chronic kidney disease: findings from the chronic renal insufficiency cohort study.
    Authors: Wing M, Yang W, Teal V, Navaneethan S, Tao K, Ojo A, Guzman N, Reilly M, Wolman M, Rosas S, Cuevas M, Fischer M, Lustigova E, Master S, Xie D, Appleby D, Joffe M, Kusek J, Feldman H, Raj D
    Obesity (Silver Spring), 2014;22(5):1359-66.
    Species: Human
    Sample Type: Plasma
  45. High-salt intake induced visceral adipose tissue hypoxia and its association with circulating monocyte subsets in humans.
    Authors: Zhou X, Yuan F, Ji W, Guo Z, Zhang L, Lu R, Liu X, Liu H, Zhang W, Jiang T, Zhang Z, Li Y
    Obesity (Silver Spring), 2014;22(6):1470-6.
    Species: Human
    Sample Type: Serum
  46. Circulating mitochondrial DNA increases with age and is a familiar trait: Implications for "inflamm-aging"
    Authors: Pinti M, Cevenini E, Nasi M, De Biasi S, Salvioli S, Monti D, Benatti S, Gibellini L, Cotichini R, Stazi M, Trenti T, Franceschi C, Cossarizza A
    Eur J Immunol, 2014;44(5):1552-62.
    Species: Human
    Sample Type: Plasma
  47. The impact of two different transfusion strategies on patient immune response during major abdominal surgery: a preliminary report.
    Authors: Theodoraki K, Markatou M, Rizos D, Fassoulaki A
    J Immunol Res, 2014;2014(0):945829.
    Species: Human
    Sample Type: Plasma
  48. Role of diacylglycerol activation of PKCtheta in lipid-induced muscle insulin resistance in humans.
    Authors: Szendroedi J, Yoshimura T, Phielix E, Koliaki C, Marcucci M, Zhang D, Jelenik T, Muller J, Herder C, Nowotny P, Shulman G, Roden M
    Proc Natl Acad Sci U S A, 2014;111(26):9597-602.
    Species: Human
    Sample Type: Plasma
  49. Threonyl-tRNA synthetase overexpression correlates with angiogenic markers and progression of human ovarian cancer.
    Authors: Wellman T, Eckenstein M, Wong C, Rincon M, Ashikaga T, Mount S, Francklyn C, Lounsbury K
    BMC Cancer, 2014;14(0):620.
    Species: Human
    Sample Type: Serum
  50. Zinc supplementation improves anticancer activity of monocytes in type-2 diabetic patients with metabolic syndrome.
    Authors: Meksawan K, Sermsri U, Chanvorachote P
    Anticancer Res, 2014;34(1):295-9.
    Species: Human
    Sample Type: Plasma
  51. Association between traffic-related air pollution, subclinical inflammation and impaired glucose metabolism: results from the SALIA study.
    Authors: Teichert T, Vossoughi M, Vierkotter A, Sugiri D, Schikowski T, Schulte T, Roden M, Luckhaus C, Herder C, Kramer U
    PLoS ONE, 2013;8(12):e83042.
    Species: Human
    Sample Type: Plasma
  52. Fractalkine is an independent predictor of mortality in patients with advanced heart failure.
    Thromb Haemost, 2012;108(6):1220-7.
    Species: Human
    Sample Type: Plasma
  53. A randomized trial of recombinant human granulocyte-macrophage colony stimulating factor for patients with acute lung injury.
    Authors: Paine R, Standiford TJ, Dechert RE, Moss M, Martin GS, Rosenberg AL, Thannickal VJ, Burnham EL, Brown MB, Hyzy RC
    Crit. Care Med., 2012;40(1):90-7.
    Species: Human
    Sample Type: BALF
  54. Multiplexing immunoassays for cytokine detection in the serum of patients with rheumatoid arthritis: lack of sensitivity and interference by rheumatoid factor.
    Authors: Churchman SM, Geiler J, Parmar R, Horner EA, Church LD, Emery P, Buch MH, McDermott MF, Ponchel F
    Clin. Exp. Rheumatol., 2012;30(4):534-42.
    Species: Human
    Sample Type: Serum
  55. Engulfment of hematopoietic stem cells caused by down-regulation of CD47 is critical in the pathogenesis of hemophagocytic lymphohistiocytosis.
    Authors: Kuriyama T, Takenaka K, Kohno K, Yamauchi T, Daitoku S, Yoshimoto G, Kikushige Y, Kishimoto J, Abe Y, Harada N, Miyamoto T, Iwasaki H, Teshima T, Akashi K
    Blood, 2012;120(19):4058-67.
    Species: Human
    Sample Type: Serum
  56. Adipocytokine levels mark endothelial function in normotensive individuals.
    Authors: Solini A, Stea F, Santini E, Bruno R, Duranti E, Taddei S, Ghiadoni L
    Cardiovasc Diabetol, 2012;11(0):103.
    Species: Human
    Sample Type: Plasma
  57. Effects of dietary fat modification on insulin sensitivity and on other risk factors of the metabolic syndrome-LIPGENE: a European randomized dietary intervention study.
    Authors: Tierney AC, McMonagle J, Shaw DI, Gulseth HL, Helal O, Saris WH, Paniagua JA, Golabek-Leszczynska I, Defoort C, Williams CM, Karsltrom B, Vessby B, Dembinska-Kiec A, Lopez-Miranda J, Blaak EE, Drevon CA, Gibney MJ, Lovegrove JA, Roche HM
    Int J Obes (Lond), 2011;35(6):800-9.
    Species: Human
    Sample Type: Serum
  58. Time-course of changes in inflammatory response after whole-body cryotherapy multi exposures following severe exercise.
    Authors: Pournot H, Bieuzen F, Louis J, Fillard JR, Barbiche E, Hausswirth C
    PLoS ONE, 2011;6(7):e22748.
    Species: Human
    Sample Type: Plasma
  59. Mechanisms inducing low bone density in duchenne muscular dystrophy in mice and humans.
    Authors: Rufo A, Del Fattore A, Capulli M, Carvello F, De Pasquale L, Ferrari S, Pierroz D, Morandi L, De Simone M, Rucci N, Bertini E, Bianchi ML, De Benedetti F, Teti A
    J. Bone Miner. Res., 2011;26(8):1891-903.
    Species: Human
    Sample Type: Serum
  60. SIRT1 mRNA expression may be associated with energy expenditure and insulin sensitivity.
    Authors: Rutanen J, Yaluri N, Modi S, Pihlajamaki J, Vanttinen M, Itkonen P, Kainulainen S, Yamamoto H, Lagouge M, Sinclair DA, Elliott P, Westphal C, Auwerx J, Laakso M
    Diabetes, 2010;59(4):829-35.
    Species: Human
    Sample Type: Plasma
  61. Sensitive plasma protein analysis by microparticle-based proximity ligation assays.
    Authors: Darmanis S, Nong RY, Hammond M, Gu J, Alderborn A, Vanelid J, Siegbahn A, Gustafsdottir S, Ericsson O, Landegren U, Kamali-Moghaddam M
    Mol. Cell Proteomics, 2010;9(2):327-35.
    Species: Human
    Sample Type: Plasma
  62. Procalcitonin as an early marker of bacterial infection in neutropenic febrile children with acute lymphoblastic leukemia.
    Authors: Hatzistilianou M, Rekliti A, Athanassiadou F, Catriu D
    Inflamm. Res., 2010;59(5):339-47.
    Species: Human
    Sample Type: Serum
  63. Changes in cytokine levels during acute hyperinsulinemia in offspring of type 2 diabetic subjects.
    Authors: Ruotsalainen E, Stancakova A, Vauhkonen I, Salmenniemi U, Pihlajamaki J, Punnonen K, Laakso M
    Atherosclerosis, 2010;210(2):536-41.
    Species: Human
    Sample Type: Plasma
  64. B7-h3 augments the inflammatory response and is associated with human sepsis.
    Authors: Zhang G, Wang J, Kelly J
    J. Immunol., 2010;185(6):3677-84.
    Species: Human
    Sample Type: Plasma
  65. Prehospital resuscitation with hypertonic saline-dextran modulates inflammatory, coagulation and endothelial activation marker profiles in severe traumatic brain injured patients.
    Authors: Rhind SG, Crnko NT, Baker AJ
    J Neuroinflammation, 2010;7(0):5.
    Species: Human
    Sample Type: Serum
  66. Differential adaptation of human gut microbiota to bariatric surgery-induced weight loss: links with metabolic and low-grade inflammation markers.
    Authors: Furet JP, Kong LC, Tap J, Poitou C, Basdevant A, Bouillot JL, Mariat D, Corthier G, Dore J, Henegar C, Rizkalla S, Clement K
    Diabetes, 2010;59(12):3049-57.
    Species: Human
    Sample Type: Plasma
  67. Nonalcoholic fatty liver disease as the transducer of hepatic oversecretion of very-low-density lipoprotein-apolipoprotein B-100 in obesity.
    Authors: Chan DC, Watts GF, Gan S
    Arterioscler. Thromb. Vasc. Biol., 2010;30(5):1043-50.
    Species: Human
    Sample Type: Plasma
  68. Adiposity and gingival crevicular fluid tumour necrosis factor-alpha levels in children.
    Authors: Khosravi R, Tran SD, Lambert M, O'Loughlin J, Kâ K, Feine JS, Caron C, Tremblay A, Nicolau B
    J. Clin. Periodontol., 2009;36(4):301-7.
    Species: Human
    Sample Type: Gingival Crevicular Fluid (GCF)
  69. Age-dependent mobilization of circulating endothelial progenitor cells in infants and young children undergoing cardiac surgery with cardiopulmonary bypass.
    Authors: Sun Y, Yi D, Wang Y, Zheng R, Sun G, Wang J, Liu Y, Ren J, Wang Y, Zhang S, Gu C, Pei J
    Cytokine, 2009;47(3):206-13.
    Species: Human
    Sample Type: Plasma
  70. Characteristics of dengue virus-infected peripheral blood mononuclear cell death that correlates with the severity of illness.
    Authors: Jaiyen Y, Masrinoul P, Kalayanarooj S, Pulmanausahakul R, Ubol S
    Microbiol. Immunol., 2009;53(8):442-50.
    Species: Human
    Sample Type: Plasma
  71. Smoking, inflammatory patterns and postprandial hypertriglyceridemia.
    Authors: Kabagambe EK, Ordovas JM, Tsai MY, Borecki IB, Hopkins PN, Glasser SP, Arnett DK
    Atherosclerosis, 2009;203(2):633-9.
    Species: Human
    Sample Type: Serum
  72. Interleukin-6 is a significant predictor of radiographic knee osteoarthritis: The Chingford Study.
    Authors: Livshits G, Zhai G, Hart DJ, Kato BS, Wang H, Williams FM, Spector TD
    Arthritis Rheum., 2009;60(7):2037-45.
    Species: Human
    Sample Type: Serum
  73. Interleukin-18 is a strong predictor of cardiovascular events in elderly men with the metabolic syndrome: synergistic effect of inflammation and hyperglycemia.
    Authors: Troseid M, Seljeflot I, Hjerkinn EM, Arnesen H
    Diabetes Care, 2009;32(3):486-92.
    Species: Human
    Sample Type: Serum
  74. Urinary proinflammatory cytokine response in renal transplant recipients with polyomavirus BK viruria.
    Authors: Sadeghi M, Daniel V, Schnitzler P, Lahdou I, Naujokat C, Zeier M, Opelz G
    Transplantation, 2009;88(9):1109-16.
    Species: Human
    Sample Type: Urine
  75. Acetaldehyde stimulates monocyte adhesion in a P-selectin- and TNFalpha-dependent manner.
    Authors: Redmond EM, Morrow D, Kundimi S, Miller-Graziano CL, Cullen JP
    Atherosclerosis, 2009;204(2):372-80.
    Species: Human
    Sample Type: Cell Culture Supernates
  76. Resistin, adiponectin, and risk of heart failure the Framingham offspring study.
    Authors: Frankel DS, Vasan RS, D'Agostino RB, Benjamin EJ, Levy D, Wang TJ, Meigs JB
    J. Am. Coll. Cardiol., 2009;53(9):754-62.
    Species: Human
    Sample Type: Plasma
  77. The proinflammatory environment in potential heart and lung donors: prevalence and impact of donor management and hormonal therapy.
    Authors: Venkateswaran RV, Dronavalli V, Lambert PA, Steeds RP, Wilson IC, Thompson RD, Mascaro JG, Bonser RS
    Transplantation, 2009;88(4):582-8.
    Species: Human
    Sample Type: Serum
  78. Intervention of an inflammation amplifier, triggering receptor expressed on myeloid cells 1, for treatment of autoimmune arthritis.
    Authors: Murakami Y, Akahoshi T, Aoki N, Toyomoto M, Miyasaka N, Kohsaka H
    Arthritis Rheum., 2009;60(6):1615-23.
    Species: Human
    Sample Type: Serum
  79. Serum CXCL13 positively correlates with prostatic disease, prostate-specific antigen and mediates prostate cancer cell invasion, integrin clustering and cell adhesion.
    Authors: Singh S, Singh R, Sharma PK, Singh UP, Rai SN, Chung LW, Cooper CR, Novakovic KR, Grizzle WE, Lillard JW
    Cancer Lett., 2009;283(1):29-35.
    Species: Human
    Sample Type: Serum
  80. Sex differences in inflammatory markers: what is the contribution of visceral adiposity?
    Authors: Cartier A, Cote M, Lemieux I, Perusse L, Tremblay A, Bouchard C, Despres JP
    Am. J. Clin. Nutr., 2009;89(5):1307-14.
    Species: Human
    Sample Type: Plasma
  81. Immunomodulation by chronobiologically-based glucocorticoids treatment for multiple sclerosis relapses.
    Authors: Glass-Marmor L, Paperna T, Galboiz Y, Miller A
    J. Neuroimmunol., 2009;210(1):124-7.
    Species: Human
    Sample Type: Serum
  82. Duffy (Fy), DARC, and neutropenia among women from the United States, Europe and the Caribbean.
    Authors: Grann VR, Ziv E, Joseph CK, Neugut AI, Wei Y, Jacobson JS, Horwitz MS, Bowman N, Beckmann K, Hershman DL
    Br. J. Haematol., 2008;143(2):288-93.
    Species: Human
    Sample Type: Plasma
  83. Duffy antigen modifies the chemokine response in human endotoxemia.
    Authors: Mayr FB, Spiel AO, Leitner JM, Firbas C, Kliegel T, Jilma-Stohlawetz P, Derendorf H, Jilma B
    Crit. Care Med., 2008;36(1):159-65.
    Species: Human
    Sample Type: Plasma
  84. In vivo transmigrated monocytes from patients with stable coronary artery disease have a reduced expression of CD11b.
    Authors: Paulsson JM, Dadfar E, Held C, Jacobson SH, Lundahl J
    Clin. Exp. Immunol., 2008;153(2):196-204.
    Species: Human
    Sample Type: Serum
  85. Neutropenia in 6 ethnic groups from the Caribbean and the U.S.
    Authors: Grann VR, Bowman N, Joseph C, Wei Y, Horwitz MS, Jacobson JS, Santella RP, Hershman DL
    Cancer, 2008;113(4):854-60.
    Species: Human
    Sample Type: Plasma
  86. Comparing Type D personality and older age as correlates of tumor necrosis factor-alpha dysregulation in chronic heart failure.
    Authors: Denollet J, Vrints CJ, Conraads VM
    Brain Behav. Immun., 2008;22(5):736-43.
    Species: Human
    Sample Type: Plasma
  87. Effects of atorvastatin added to inhaled corticosteroids on lung function and sputum cell counts in atopic asthma.
    Authors: Hothersall EJ, Chaudhuri R, McSharry C, Donnelly I, Lafferty J, McMahon AD, Weir CJ, Meiklejohn J, Sattar N, McInnes I, Wood S, Thomson NC
    Thorax, 2008;63(12):1070-5.
    Species: Human
    Sample Type: Serum
  88. Identification of interleukin-7 as a candidate disease mediator in spondylarthritis.
    Authors: Rihl M, Kellner H, Kellner W, Barthel C, Yu DT, Tak PP, Zeidler H, Baeten D
    Arthritis Rheum., 2008;58(11):3430-5.
    Species: Human
    Sample Type: Synovial Fluid
  89. Low-dose acetylsalicylic acid inhibits the secretion of interleukin-6 from white adipose tissue.
    Authors: Ogston NC, Karastergiou K, Hosseinzadeh-Attar MJ, Bhome R, Madani R, Stables M, Gilroy D, Flachs P, Hensler M, Kopecky J, Mohamed-Ali V
    Int J Obes (Lond), 2008;32(12):1807-15.
    Species: Human
    Sample Type: Plasma
  90. Self-esteem levels and cardiovascular and inflammatory responses to acute stress.
    Authors: O'Donnell K, Brydon L, Wright CE, Steptoe A
    Brain Behav. Immun., 2008;0(0):.
    Species: Human
    Sample Type: Plasma
  91. Effects of rehabilitative exercise on peripheral muscle TNFalpha, IL-6, IGF-I and MyoD expression in patients with COPD.
    Authors: Vogiatzis I, Stratakos G, Simoes DC, Terzis G, Georgiadou O, Roussos C, Zakynthinos S
    Thorax, 2007;62(11):950-6.
    Species: Human
    Sample Type: Plasma
  92. Co-occurrence of IgA antibodies against ethanol metabolites and tissue transglutaminase in alcohol consumers: correlation with proinflammatory cytokines and markers of fibrogenesis.
    Authors: Koivisto H, Hietala J, Anttila P, Niemela O
    Dig. Dis. Sci., 2007;53(2):500-5.
    Species: Human
    Sample Type: Serum
  93. Cerebrospinal fluid IL-6, HSP72, and TNF-alpha in exercising humans.
    Authors: Steensberg A, Dalsgaard MK, Secher NH, Pedersen BK
    Brain Behav. Immun., 2006;20(6):585-9.
    Species: Human
    Sample Type: Plasma
  94. The activity of N-acetyl-beta-D-glucosaminidase and tumor necrosis factor-alpha at early stage of diabetic retinopathy development in type 1 diabetes mellitus children.
    Authors: Mysliwiec M, Zorena K, Balcerska A, Mysliwska J, Lipowski P, Raczynska K
    Clin. Biochem., 2006;39(8):851-6.
    Species: Human
    Sample Type: Serum
  95. Postprandial total and HMW adiponectin following a high-fat meal in lean, obese and diabetic men.
    Authors: Phillips L, Peake J, Zhang X, Hickman I, Briskey D, Huang B, Simpson P, Li S, Whitehead J, Martin J, Prins J
    Eur J Clin Nutr, 0;67(4):377-84.
    Species: Human
    Sample Type: Plasma
Expand to show all Citations

FAQs

No product specific FAQs exist for this product, however you may

View all ELISA FAQs
ELISA Controls
Description Application Cat# Citations Images  

Quantikine Immunoassay Control Group 232

Ctrl QC232
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Average Rating: 5 (Based on 3 reviews)

Have you used Human TNF-alpha Quantikine HS ELISA?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Read what people are saying who have used Human TNF-alpha Quantikine HS ELISA.

Filter by Ratings
Excellent
(3)
All Ratings
Filter by Applications
All Applications
Filter by Species
All Species

Images Ratings Applications Species Reviewed By Date Details
  Excellent
  Anonymous 01/18/2018
Show

Summary

Sample Tested EDTA Plasma
 Human TNF-alpha Quantikine HS ELISA HSTA00D Array HSTA00D
Enlarge
Excellent
  Anonymous 08/04/2017
Show
Image Details
 Human TNF-alpha Quantikine HS ELISA HSTA00D
: Human TNF-alpha Quantikine HS ELISA [HSTA00D].

Summary

Sample Tested Peripheral blood neutrophils

Other Experimental Details

Other Experimental Details Due to the short experimental time, the standard TNFa duoSET ELISA did not work, however HS kit did.
 Human TNF-alpha Quantikine HS ELISA HSTA00D Array HSTA00D
Enlarge
Excellent
  Anonymous 05/25/2016
Show
Image Details
 Human TNF-alpha Quantikine HS ELISA HSTA00D
: Human TNF-alpha Quantikine HS ELISA [HSTA00D].

Summary

Sample Tested Human serum

Please scroll the above reviews for more
Contact Us to Order
reload

Feedback

Customer Information
R&D Systems Guarantee