hh Human TNF-alpha Quantikine HS ELISA: R&D Systems

Human TNF-alpha Quantikine HS ELISA

HSTA00D has been discontinued and is replaced by HSTA00E.
Product is in the process of discontinuation and replaced by catalog # HSTA00E.
(81 citations)
(2 Reviews)
  
  • Assay Length
    4 hours
  • Sample Type & Volume Required Per Well
    Serum (200 uL), EDTA Plasma (200 uL), Heparin Plasma (200 uL), Citrate Plasma (200 uL)
  • Sensitivity
    0.191 pg/mL
  • Assay Range
    0.5 - 32 pg/mL (Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
  • Specificity
    Natural and recombinant human TNF-alpha
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Product Summary
The Quantikine HS Human TNF-alpha Immunoassay is a 6.5 hour solid phase ELISA designed to measure TNF-alpha in serum and plasma. It contains E. coli-derived recombinant human TNF-alpha and antibodies raised against the recombinant factor. It has been shown to accurately quantitate recombinant human TNF-alpha. Results obtained with naturally occurring TNF-alpha samples showed linear curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural TNF-alpha. Since the measurement of TNF-alpha is insensitive to the addition of recombinant forms of either of the two types of soluble receptors, it is probable that this measurement detects the total amount of TNF-alpha in samples, i.e., the total amount of free TNF-alpha plus the amount of TNF-alpha bound to soluble receptors.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020414141
Mean1.9611.522.11.8310.520.3
Standard Deviation0.170.490.680.190.761.5
CV%8.74.33.110.47.27.4

Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020202020
Mean1.073.36.711.093.266.51
Standard Deviation0.0240.0660.1290.0730.220.406
CV%2.221.96.76.76.2

Recovery

The recovery of TNF-alpha was determined by spiking to three different levels throughout the range of the assay in various matrices.

Sample Type Average % Recovery Range %
Citrate Plasma (n=4) 95 88-105
Citrate Plasma (n=4) 95 88-105
Citrate Plasma (n=4) 95 88-105
Citrate Plasma (n=4) 95 88-105
EDTA Plasma (n=4) 99 92-111
EDTA Plasma (n=4) 99 92-111
EDTA Plasma (n=4) 99 92-111
EDTA Plasma (n=4) 99 92-111
Heparin Plasma (n=4) 93 87-103
Heparin Plasma (n=4) 93 87-103
Heparin Plasma (n=4) 93 87-103
Heparin Plasma (n=4) 93 87-103
Serum (n=4) 93 85-98
Serum (n=4) 93 85-98
Serum (n=4) 93 85-98
Serum (n=4) 93 85-98
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of TNF-alpha and diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human TNF-alpha Quantikine HS ELISA
Human TNF-alpha Quantikine HS ELISA
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: TNF-alpha

Tumor necrosis factor alpha (TNF-α), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, apoptosis, and immune system development. TNF-α is produced by a wide variety of immune and epithelial cell types. Human TNF-α consists of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 177 aa extracellular domain (ECD). Within the ECD, human TNF-α shares 97% aa sequence identity with rhesus and 71% - 92% with bovine, canine, cotton rat, equine, feline, mouse, porcine, and rat TNF-α. The 26 kDa type 2 transmembrane protein is assembled intracellularly to form a noncovalently linked homotrimer. Ligation of this complex induces reverse signaling that promotes lymphocyte costimulation but diminishes monocyte responsiveness.

Cleavage of membrane bound TNF-α by TACE/ADAM17 releases a 55 kDa soluble trimeric form of TNF-α. TNF-α trimers bind the ubiquitous TNF RI and the hematopoietic cell-restricted TNF RII, both of which are also expressed as homotrimers. TNF-α regulates lymphoid tissue development through control of apoptosis. It also promotes inflammatory responses by inducing the activation of vascular endothelial cells and macrophages. TNF-α is a key cytokine in the development of several inflammatory disorders. It contributes to the development of type 2 diabetes through its effects on insulin resistance and fatty acid metabolism.

    • Long Name
      Tumor Necrosis Factor alpha
    • Entrez Gene IDs
      7124 (Human); 21926 (Mouse); 24835 (Rat); 397086 (Porcine); 280943 (Bovine); 403922 (Canine); 100033834 (Equine); 493755 (Feline); 100009088 (Rabbit);
    • Alternate Names
      APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; tnfa; tnf-a; TNFalpha; TNF-alpha; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor-alpha;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 50 µL Assay Diluent
    4.   Add 50 µL of Assay Diluent to each well.

    5. 200 µL Standard, Control, or Sample
    6.   Add 200 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 3 hours.
    7.   Aspirate each well and wash, repeating the process 5 times for a total of 6 washes.

    8. 200 µL Conjugate
    9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
    10.   Aspirate and wash 6 times.

    11. 50 µL Substrate Solution
    12.   Add 50 µL Substrate Solution to each well. Cover with a new plate sealer, and incubate at room temperature for 1 hour. Do not wash the plate.

    13. 50 µL Amplifier Solution
    14.   Add 50 µL Amplifier Solution to each well. Cover with a new plate sealer, and incubate at room temperature for 30 minutes.

    15. 50 µL Stop Solution
    16. Add 50 µL of Stop Solution to each well. Read at 490 nm within 30 minutes. Set wavelength correction to 650 nm or 690 nm.
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    81 Citations: Showing 1 - 10
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    Species
    Sample Type
    1. Repeated Excessive Exercise Attenuates the Anti-Inflammatory Effects of Exercise in Older Men
      Authors: RE Sahl, PR Andersen, K Gronbaek, TH Morville, M Rosenkilde, HK Rasmusen, SS Poulsen, C Prats, F Dela, JW Helge
      Front Physiol, 2017;8(0):407.
      Species: Human
      Sample Type: Plasma
    2. Association of plasma CD40L with acute chest syndrome in sickle cell anemia
      Authors: VT Garrido, L Sonzogni, SN Mtatiro, FF Costa, N Conran, SL Thein
      Cytokine, 2017;97(0):104-107.
      Species: Human
      Sample Type: Plasma
    3. Chemokine signals are crucial for enhanced homing and differentiation of circulating osteoclast progenitor cells
      Authors: A Sucur, Z Jajic, M Artukovic, MI Matijasevi, B Anic, D Flegar, A Markotic, T Kelava, S Ivcevic, N Kovacic, V Katavic, D Grcevic
      Arthritis Res. Ther., 2017;19(1):142.
      Species: Human
      Sample Type: Plasma
    4. Microenvironment inflammatory infiltrate drives growth speed and outcome of hepatocellular carcinoma: a prospective clinical study
      Authors: R Critelli, F Milosa, F Faillaci, R Condello, E Turola, L Marzi, B Lei, F Dituri, S Andreani, P Sighinolfi, P Manni, A Maiorana, C Caporali, F di Benedet, M Del Buono, N De Maria, F Schepis, ML Martinez-C, G Giannelli, E Villa
      Cell Death Dis, 2017;8(8):e3017.
      Species: Human
      Sample Type: Serum
    5. Visceral fat area is a strong predictor of leukocyte cell-derived chemotaxin 2, a potential biomarker of dyslipidemia
      Authors: K Tanisawa, H Taniguchi, X Sun, T Ito, R Kawakami, S Sakamoto, M Higuchi
      PLoS ONE, 2017;12(3):e0173310.
      Species: Human
      Sample Type: Serum
    6. Anti-TNF-? Drugs Differently Affect the TNF?-sTNFR System and Monocyte Subsets in Patients with Psoriasis
      PLoS ONE, 2016;11(12):e0167757.
    7. Interleukin-6 Is a Risk Factor for Atrial Fibrillation in Chronic Kidney Disease: Findings from the CRIC Study.
      Authors: Amdur R, Mukherjee M, Go A, Barrows I, Ramezani A, Shoji J, Reilly M, Gnanaraj J, Deo R, Roas S, Keane M, Master S, Teal V, Soliman E, Yang P, Feldman H, Kusek J, Tracy C, Raj D
      PLoS ONE, 2016;11(2):e0148189.
      Species: Human
      Sample Type: Plasma
    8. Effects of nateglinide and rosiglitazone on pancreatic alpha- and beta-cells, GLP-1 secretion and inflammatory markers in patients with type 2 diabetes: randomized crossover clinical study.
      Authors: Tostes G, Cunha M, Fukui R, Correia M, Rocha D, Dos Santos R, da Silva M
      Diabetol Metab Syndr, 2016;8(0):1.
      Species: Human
      Sample Type: Serum
    9. Local TNF causes NFATc1-dependent cholesterol-mediated podocyte injury
      J Clin Invest, 2016;0(0):.
      Species: Human
      Sample Type: Serum
    10. Reduction of c-Fos via Overexpression of miR-34a Results in Enhancement of TNF- Production by LPS in Neutrophils from Myelodysplastic Syndrome Patients
      PLoS ONE, 2016;11(8):e0158527.
      Species: Human
      Sample Type: Cell Culture Supernates
    11. Trait Hostility and Acute Inflammatory Responses to Stress in the Laboratory
      Authors: Dominique Girard
      PLoS ONE, 2016;11(6):e0156329.
      Species: Human
      Sample Type: Serum
    12. Casein glycomacropeptide for active distal ulcerative colitis: a randomised pilot study
      Authors: CL Hvas, A Dige, M Bendix, PG Wernlund, LA Christense, JF Dahlerup, J Agnholt
      Eur J Clin Invest, 2016;0(0):.
      Species: Human
      Sample Type: Plasma
    13. Circadian misalignment increases cardiovascular disease risk factors in humans
      Proc. Natl. Acad. Sci. U.S.A., 2016;113(10):E1402-11.
      Species: Human
      Sample Type: Serum
    14. The relationship between circulating neutrophil gelatinase-associated lipocalin and early alteration of metabolic parameters is associated with dietary saturated fat intake in non-diabetic Korean women
      Authors: GY Na, SR Yoon, J An, R Yeo, J Song, MN Jo, S Han, OY Kim
      Endocr. J, 2016;0(0):.
      Species: Human
      Sample Type: Plasma
    15. A randomized, placebo controlled trial of omega-3 fatty acids in the treatment of young children with autism.
      Authors: Mankad D, Dupuis A, Smile S, Roberts W, Brian J, Lui T, Genore L, Zaghloul D, Iaboni A, Marcon P, Anagnostou E
      Mol Autism, 2015;6(0):18.
      Species: Human
      Sample Type: Plasma
    16. Sclerostin, TNF-alpha and Interleukin-18 Correlate and are Together with Klotho Related to Other Growth Factors and Cytokines in Haemodialysis Patients.
      Authors: Almroth G, Lonn J, Uhlin F, Brudin L, Andersson B, Hahn-Zoric M
      Scand J Immunol, 2015;83(1):58-63.
      Species: Human
      Sample Type: Serum
    17. Differential Patterns and Determinants of Cardiac Autonomic Nerve Dysfunction during Endotoxemia and Oral Fat Load in Humans.
      Authors: Ziegler D, Strom A, Strassburger K, Nowotny B, Zahiragic L, Nowotny P, Carstensen-Kirberg M, Herder C, Szendroedi J, Roden M
      PLoS ONE, 2015;10(4):e0124242.
      Species: Human
      Sample Type: Serum
    18. Plasma TNF-alpha and Soluble TNF Receptor Levels after Doxorubicin with or without Co-Administration of Mesna-A Randomized, Cross-Over Clinical Study.
      Authors: Hayslip J, Dressler E, Weiss H, Taylor T, Chambers M, Noel T, Miriyala S, Keeney J, Ren X, Sultana R, Vore M, Butterfield D, St Clair D, Moscow J
      PLoS ONE, 2015;10(4):e0124988.
      Species: Human
      Sample Type: Plasma
    19. Effects of Acute Endurance Exercise Performed in the Morning and Evening on Inflammatory Cytokine and Metabolic Hormone Responses.
      Authors: Kim H, Konishi M, Takahashi M, Tabata H, Endo N, Numao S, Lee S, Kim Y, Suzuki K, Sakamoto S
      PLoS ONE, 2015;10(9):e0137567.
      Species: Human
      Sample Type: Plasma
    20. Association of Sarcopenic Obesity with Higher Serum High-Sensitivity C-Reactive Protein Levels in Chinese Older Males--A Community-Based Study (Taichung Community Health Study-Elderly, TCHS-E).
      Authors: Yang C, Li C, Li T, Liu C, Lin C, Lin W, Lin C
      PLoS ONE, 2015;10(7):e0132908.
      Species: Human
      Sample Type: Serum
    21. Disturbances in Hypothalamic-Pituitary-Adrenal Axis and Immunological Activity Differentiating between Unipolar and Bipolar Depressive Episodes.
      Authors: Becking K, Spijker A, Hoencamp E, Penninx B, Schoevers R, Boschloo L
      PLoS ONE, 2015;10(7):e0133898.
      Species: Human
      Sample Type: Plasma
    22. Tumor necrosis factor-alpha confers cardioprotection through ectopic expression of keratins K8 and K18.
      Authors: Papathanasiou S, Rickelt S, Soriano M, Schips T, Maier H, Davos C, Varela A, Kaklamanis L, Mann D, Capetanaki Y
      Nat Med, 2015;21(9):1076-84.
      Species: Human
      Sample Type: Serum
    23. Effects of pentoxifylline on proteinuria and glucose control in patients with type 2 diabetes: a prospective randomized double-blind multicenter study.
      Authors: Han S, Kim H, Kim D, Sheen S, Chung C, Ahn C, Kim S, Cho Y, Park S, Kim S, Kim C, Kim K, Lee K
      Diabetol Metab Syndr, 2015;7(0):64.
      Species: Human
      Sample Type: Serum
    24. Association between Inflammation and Cardiac Geometry in Chronic Kidney Disease: Findings from the CRIC Study.
      Authors: Gupta J, Dominic E, Fink J, Ojo A, Barrows I, Reilly M, Townsend R, Joffe M, Rosas S, Wolman M, Patel S, Keane M, Feldman H, Kusek J, Raj D
      PLoS ONE, 2015;10(4):e0124772.
      Species: Human
      Sample Type: Plasma
    25. Tumor-specific cytotoxic T lymphocyte activity determines colorectal cancer patient prognosis.
      Authors: Reissfelder C, Stamova S, Gossmann C, Braun M, Bonertz A, Walliczek U, Grimm M, Rahbari N, Koch M, Saadati M, Benner A, Buchler M, Jager D, Halama N, Khazaie K, Weitz J, Beckhove P
      J Clin Invest, 2015;125(2):739-51.
      Species: Human
      Sample Type: Tissue Homogenates
    26. Smac mimetics and innate immune stimuli synergize to promote tumor death.
      Authors: Beug S, Tang V, Lacasse E, Cheung H, Beauregard C, Brun J, Nuyens J, Earl N, St-Jean M, Holbrook J, Dastidar H, Mahoney D, Ilkow C, Le Boeuf F, Bell J, Korneluk R
      Nat Biotechnol, 2014;32(2):182-90.
      Species: Human
      Sample Type: Cell Culture Supernates
    27. The impact of two different transfusion strategies on patient immune response during major abdominal surgery: a preliminary report.
      Authors: Theodoraki K, Markatou M, Rizos D, Fassoulaki A
      J Immunol Res, 2014;2014(0):945829.
      Species: Human
      Sample Type: Plasma
    28. Race modifies the association between adiposity and inflammation in patients with chronic kidney disease: findings from the chronic renal insufficiency cohort study.
      Authors: Wing M, Yang W, Teal V, Navaneethan S, Tao K, Ojo A, Guzman N, Reilly M, Wolman M, Rosas S, Cuevas M, Fischer M, Lustigova E, Master S, Xie D, Appleby D, Joffe M, Kusek J, Feldman H, Raj D
      Obesity (Silver Spring), 2014;22(5):1359-66.
      Species: Human
      Sample Type: Plasma
    29. High-salt intake induced visceral adipose tissue hypoxia and its association with circulating monocyte subsets in humans.
      Authors: Zhou X, Yuan F, Ji W, Guo Z, Zhang L, Lu R, Liu X, Liu H, Zhang W, Jiang T, Zhang Z, Li Y
      Obesity (Silver Spring), 2014;22(6):1470-6.
      Species: Human
      Sample Type: Serum
    30. Circulating mitochondrial DNA increases with age and is a familiar trait: Implications for "inflamm-aging"
      Authors: Pinti M, Cevenini E, Nasi M, De Biasi S, Salvioli S, Monti D, Benatti S, Gibellini L, Cotichini R, Stazi M, Trenti T, Franceschi C, Cossarizza A
      Eur J Immunol, 2014;44(5):1552-62.
      Species: Human
      Sample Type: Plasma
    31. Compartment differences of inflammatory activity in chronic obstructive pulmonary disease.
      Authors: Ji J, von Scheele I, Bergstrom J, Billing B, Dahlen B, Lantz A, Larsson K, Palmberg L
      Respir Res, 2014;15(0):104.
      Species: Human
      Sample Type: Serum
    32. Role of diacylglycerol activation of PKCtheta in lipid-induced muscle insulin resistance in humans.
      Authors: Szendroedi J, Yoshimura T, Phielix E, Koliaki C, Marcucci M, Zhang D, Jelenik T, Muller J, Herder C, Nowotny P, Shulman G, Roden M
      Proc Natl Acad Sci U S A, 2014;111(26):9597-602.
      Species: Human
      Sample Type: Plasma
    33. Big breakfast rich in protein and fat improves glycemic control in type 2 diabetics.
      Authors: Rabinovitz H, Boaz M, Ganz T, Jakubowicz D, Matas Z, Madar Z, Wainstein J
      Obesity (Silver Spring), 2014;22(5):E46-54.
      Species: Human
      Sample Type: Plasma
    34. Zinc supplementation improves anticancer activity of monocytes in type-2 diabetic patients with metabolic syndrome.
      Authors: Meksawan K, Sermsri U, Chanvorachote P
      Anticancer Res, 2014;34(1):295-9.
      Species: Human
      Sample Type: Plasma
    35. Threonyl-tRNA synthetase overexpression correlates with angiogenic markers and progression of human ovarian cancer.
      Authors: Wellman T, Eckenstein M, Wong C, Rincon M, Ashikaga T, Mount S, Francklyn C, Lounsbury K
      BMC Cancer, 2014;14(0):620.
      Species: Human
      Sample Type: Serum
    36. The protective effect of esculentoside a on experimental acute liver injury in mice.
      Authors: Zhang F, Wang X, Qiu X, Wang J, Fang H, Wang Z, Sun Y, Xia Z
      PLoS ONE, 2014;9(11):e113107.
      Species: Human
      Sample Type: Cell Culture Supernates
    37. Association between traffic-related air pollution, subclinical inflammation and impaired glucose metabolism: results from the SALIA study.
      Authors: Teichert T, Vossoughi M, Vierkotter A, Sugiri D, Schikowski T, Schulte T, Roden M, Luckhaus C, Herder C, Kramer U
      PLoS ONE, 2013;8(12):e83042.
      Species: Human
      Sample Type: Plasma
    38. Multiplexing immunoassays for cytokine detection in the serum of patients with rheumatoid arthritis: lack of sensitivity and interference by rheumatoid factor.
      Authors: Churchman SM, Geiler J, Parmar R, Horner EA, Church LD, Emery P, Buch MH, McDermott MF, Ponchel F
      Clin. Exp. Rheumatol., 2012;30(4):534-42.
      Species: Human
      Sample Type: Serum
    39. Engulfment of hematopoietic stem cells caused by down-regulation of CD47 is critical in the pathogenesis of hemophagocytic lymphohistiocytosis.
      Authors: Kuriyama T, Takenaka K, Kohno K, Yamauchi T, Daitoku S, Yoshimoto G, Kikushige Y, Kishimoto J, Abe Y, Harada N, Miyamoto T, Iwasaki H, Teshima T, Akashi K
      Blood, 2012;120(19):4058-67.
      Species: Human
      Sample Type: Serum
    40. Fractalkine is an independent predictor of mortality in patients with advanced heart failure.
      Thromb Haemost, 2012;108(6):1220-7.
      Species: Human
      Sample Type: Plasma
    41. Adipocytokine levels mark endothelial function in normotensive individuals.
      Authors: Solini A, Stea F, Santini E, Bruno R, Duranti E, Taddei S, Ghiadoni L
      Cardiovasc Diabetol, 2012;11(0):103.
      Species: Human
      Sample Type: Plasma
    42. A randomized trial of recombinant human granulocyte-macrophage colony stimulating factor for patients with acute lung injury.
      Authors: Paine R, Standiford TJ, Dechert RE, Moss M, Martin GS, Rosenberg AL, Thannickal VJ, Burnham EL, Brown MB, Hyzy RC
      Crit. Care Med., 2012;40(1):90-7.
      Species: Human
      Sample Type: BALF
    43. Time-course of changes in inflammatory response after whole-body cryotherapy multi exposures following severe exercise.
      Authors: Pournot H, Bieuzen F, Louis J, Fillard JR, Barbiche E, Hausswirth C
      PLoS ONE, 2011;6(7):e22748.
      Species: Human
      Sample Type: Plasma
    44. Mechanisms inducing low bone density in duchenne muscular dystrophy in mice and humans.
      Authors: Rufo A, Del Fattore A, Capulli M, Carvello F, De Pasquale L, Ferrari S, Pierroz D, Morandi L, De Simone M, Rucci N, Bertini E, Bianchi ML, De Benedetti F, Teti A
      J. Bone Miner. Res., 2011;26(8):1891-903.
      Species: Human
      Sample Type: Serum
    45. Effects of dietary fat modification on insulin sensitivity and on other risk factors of the metabolic syndrome-LIPGENE: a European randomized dietary intervention study.
      Authors: Tierney AC, McMonagle J, Shaw DI, Gulseth HL, Helal O, Saris WH, Paniagua JA, Golabek-Leszczynska I, Defoort C, Williams CM, Karsltrom B, Vessby B, Dembinska-Kiec A, Lopez-Miranda J, Blaak EE, Drevon CA, Gibney MJ, Lovegrove JA, Roche HM
      Int J Obes (Lond), 2011;35(6):800-9.
      Species: Human
      Sample Type: Serum
    46. SIRT1 mRNA expression may be associated with energy expenditure and insulin sensitivity.
      Authors: Rutanen J, Yaluri N, Modi S, Pihlajamaki J, Vanttinen M, Itkonen P, Kainulainen S, Yamamoto H, Lagouge M, Sinclair DA, Elliott P, Westphal C, Auwerx J, Laakso M
      Diabetes, 2010;59(4):829-35.
      Species: Human
      Sample Type: Plasma
    47. Sensitive plasma protein analysis by microparticle-based proximity ligation assays.
      Authors: Darmanis S, Nong RY, Hammond M, Gu J, Alderborn A, Vanelid J, Siegbahn A, Gustafsdottir S, Ericsson O, Landegren U, Kamali-Moghaddam M
      Mol. Cell Proteomics, 2010;9(2):327-35.
      Species: Human
      Sample Type: Plasma
    48. Nonalcoholic fatty liver disease as the transducer of hepatic oversecretion of very-low-density lipoprotein-apolipoprotein B-100 in obesity.
      Authors: Chan DC, Watts GF, Gan S
      Arterioscler. Thromb. Vasc. Biol., 2010;30(5):1043-50.
      Species: Human
      Sample Type: Plasma
    49. Differential adaptation of human gut microbiota to bariatric surgery-induced weight loss: links with metabolic and low-grade inflammation markers.
      Authors: Furet JP, Kong LC, Tap J, Poitou C, Basdevant A, Bouillot JL, Mariat D, Corthier G, Dore J, Henegar C, Rizkalla S, Clement K
      Diabetes, 2010;59(12):3049-57.
      Species: Human
      Sample Type: Plasma
    50. Procalcitonin as an early marker of bacterial infection in neutropenic febrile children with acute lymphoblastic leukemia.
      Authors: Hatzistilianou M, Rekliti A, Athanassiadou F, Catriu D
      Inflamm. Res., 2010;59(5):339-47.
      Species: Human
      Sample Type: Serum
    51. Changes in cytokine levels during acute hyperinsulinemia in offspring of type 2 diabetic subjects.
      Authors: Ruotsalainen E, Stancakova A, Vauhkonen I, Salmenniemi U, Pihlajamaki J, Punnonen K, Laakso M
      Atherosclerosis, 2010;210(2):536-41.
      Species: Human
      Sample Type: Plasma
    52. Prehospital resuscitation with hypertonic saline-dextran modulates inflammatory, coagulation and endothelial activation marker profiles in severe traumatic brain injured patients.
      Authors: Rhind SG, Crnko NT, Baker AJ
      J Neuroinflammation, 2010;7(0):5.
      Species: Human
      Sample Type: Serum
    53. B7-h3 augments the inflammatory response and is associated with human sepsis.
      Authors: Zhang G, Wang J, Kelly J
      J. Immunol., 2010;185(6):3677-84.
      Species: Human
      Sample Type: Plasma
    54. Interleukin-6 is a significant predictor of radiographic knee osteoarthritis: The Chingford Study.
      Authors: Livshits G, Zhai G, Hart DJ, Kato BS, Wang H, Williams FM, Spector TD
      Arthritis Rheum., 2009;60(7):2037-45.
      Species: Human
      Sample Type: Serum
    55. Adiposity and gingival crevicular fluid tumour necrosis factor-alpha levels in children.
      Authors: Khosravi R, Tran SD, Lambert M, O'Loughlin J, Kâ K, Feine JS, Caron C, Tremblay A, Nicolau B
      J. Clin. Periodontol., 2009;36(4):301-7.
      Species: Human
      Sample Type: Gingival Crevicular Fluid (GCF)
    56. Characteristics of dengue virus-infected peripheral blood mononuclear cell death that correlates with the severity of illness.
      Authors: Jaiyen Y, Masrinoul P, Kalayanarooj S, Pulmanausahakul R, Ubol S
      Microbiol. Immunol., 2009;53(8):442-50.
      Species: Human
      Sample Type: Plasma
    57. Acetaldehyde stimulates monocyte adhesion in a P-selectin- and TNFalpha-dependent manner.
      Authors: Redmond EM, Morrow D, Kundimi S, Miller-Graziano CL, Cullen JP
      Atherosclerosis, 2009;204(2):372-80.
      Species: Human
      Sample Type: Cell Culture Supernates
    58. Immunomodulation by chronobiologically-based glucocorticoids treatment for multiple sclerosis relapses.
      Authors: Glass-Marmor L, Paperna T, Galboiz Y, Miller A
      J. Neuroimmunol., 2009;210(1):124-7.
      Species: Human
      Sample Type: Serum
    59. Sex differences in inflammatory markers: what is the contribution of visceral adiposity?
      Authors: Cartier A, Cote M, Lemieux I, Perusse L, Tremblay A, Bouchard C, Despres JP
      Am. J. Clin. Nutr., 2009;89(5):1307-14.
      Species: Human
      Sample Type: Plasma
    60. Intervention of an inflammation amplifier, triggering receptor expressed on myeloid cells 1, for treatment of autoimmune arthritis.
      Authors: Murakami Y, Akahoshi T, Aoki N, Toyomoto M, Miyasaka N, Kohsaka H
      Arthritis Rheum., 2009;60(6):1615-23.
      Species: Human
      Sample Type: Serum
    61. Interleukin-18 is a strong predictor of cardiovascular events in elderly men with the metabolic syndrome: synergistic effect of inflammation and hyperglycemia.
      Authors: Troseid M, Seljeflot I, Hjerkinn EM, Arnesen H
      Diabetes Care, 2009;32(3):486-92.
      Species: Human
      Sample Type: Serum
    62. Resistin, adiponectin, and risk of heart failure the Framingham offspring study.
      Authors: Frankel DS, Vasan RS, D'Agostino RB, Benjamin EJ, Levy D, Wang TJ, Meigs JB
      J. Am. Coll. Cardiol., 2009;53(9):754-62.
      Species: Human
      Sample Type: Plasma
    63. Serum CXCL13 positively correlates with prostatic disease, prostate-specific antigen and mediates prostate cancer cell invasion, integrin clustering and cell adhesion.
      Authors: Singh S, Singh R, Sharma PK, Singh UP, Rai SN, Chung LW, Cooper CR, Novakovic KR, Grizzle WE, Lillard JW
      Cancer Lett., 2009;283(1):29-35.
      Species: Human
      Sample Type: Serum
    64. Urinary proinflammatory cytokine response in renal transplant recipients with polyomavirus BK viruria.
      Authors: Sadeghi M, Daniel V, Schnitzler P, Lahdou I, Naujokat C, Zeier M, Opelz G
      Transplantation, 2009;88(9):1109-16.
      Species: Human
      Sample Type: Urine
    65. Age-dependent mobilization of circulating endothelial progenitor cells in infants and young children undergoing cardiac surgery with cardiopulmonary bypass.
      Authors: Sun Y, Yi D, Wang Y, Zheng R, Sun G, Wang J, Liu Y, Ren J, Wang Y, Zhang S, Gu C, Pei J
      Cytokine, 2009;47(3):206-13.
      Species: Human
      Sample Type: Plasma
    66. The proinflammatory environment in potential heart and lung donors: prevalence and impact of donor management and hormonal therapy.
      Authors: Venkateswaran RV, Dronavalli V, Lambert PA, Steeds RP, Wilson IC, Thompson RD, Mascaro JG, Bonser RS
      Transplantation, 2009;88(4):582-8.
      Species: Human
      Sample Type: Serum
    67. Smoking, inflammatory patterns and postprandial hypertriglyceridemia.
      Authors: Kabagambe EK, Ordovas JM, Tsai MY, Borecki IB, Hopkins PN, Glasser SP, Arnett DK
      Atherosclerosis, 2009;203(2):633-9.
      Species: Human
      Sample Type: Serum
    68. In vivo transmigrated monocytes from patients with stable coronary artery disease have a reduced expression of CD11b.
      Authors: Paulsson JM, Dadfar E, Held C, Jacobson SH, Lundahl J
      Clin. Exp. Immunol., 2008;153(2):196-204.
      Species: Human
      Sample Type: Serum
    69. Effects of atorvastatin added to inhaled corticosteroids on lung function and sputum cell counts in atopic asthma.
      Authors: Hothersall EJ, Chaudhuri R, McSharry C, Donnelly I, Lafferty J, McMahon AD, Weir CJ, Meiklejohn J, Sattar N, McInnes I, Wood S, Thomson NC
      Thorax, 2008;63(12):1070-5.
      Species: Human
      Sample Type: Serum
    70. Low-dose acetylsalicylic acid inhibits the secretion of interleukin-6 from white adipose tissue.
      Authors: Ogston NC, Karastergiou K, Hosseinzadeh-Attar MJ, Bhome R, Madani R, Stables M, Gilroy D, Flachs P, Hensler M, Kopecky J, Mohamed-Ali V
      Int J Obes (Lond), 2008;32(12):1807-15.
      Species: Human
      Sample Type: Plasma
    71. Duffy (Fy), DARC, and neutropenia among women from the United States, Europe and the Caribbean.
      Authors: Grann VR, Ziv E, Joseph CK, Neugut AI, Wei Y, Jacobson JS, Horwitz MS, Bowman N, Beckmann K, Hershman DL
      Br. J. Haematol., 2008;143(2):288-93.
      Species: Human
      Sample Type: Plasma
    72. Duffy antigen modifies the chemokine response in human endotoxemia.
      Authors: Mayr FB, Spiel AO, Leitner JM, Firbas C, Kliegel T, Jilma-Stohlawetz P, Derendorf H, Jilma B
      Crit. Care Med., 2008;36(1):159-65.
      Species: Human
      Sample Type: Plasma
    73. Self-esteem levels and cardiovascular and inflammatory responses to acute stress.
      Authors: O'Donnell K, Brydon L, Wright CE, Steptoe A
      Brain Behav. Immun., 2008;0(0):.
      Species: Human
      Sample Type: Plasma
    74. Neutropenia in 6 ethnic groups from the Caribbean and the U.S.
      Authors: Grann VR, Bowman N, Joseph C, Wei Y, Horwitz MS, Jacobson JS, Santella RP, Hershman DL
      Cancer, 2008;113(4):854-60.
      Species: Human
      Sample Type: Plasma
    75. Comparing Type D personality and older age as correlates of tumor necrosis factor-alpha dysregulation in chronic heart failure.
      Authors: Denollet J, Vrints CJ, Conraads VM
      Brain Behav. Immun., 2008;22(5):736-43.
      Species: Human
      Sample Type: Plasma
    76. Identification of interleukin-7 as a candidate disease mediator in spondylarthritis.
      Authors: Rihl M, Kellner H, Kellner W, Barthel C, Yu DT, Tak PP, Zeidler H, Baeten D
      Arthritis Rheum., 2008;58(11):3430-5.
      Species: Human
      Sample Type: Synovial Fluid
    77. Effects of rehabilitative exercise on peripheral muscle TNFalpha, IL-6, IGF-I and MyoD expression in patients with COPD.
      Authors: Vogiatzis I, Stratakos G, Simoes DC, Terzis G, Georgiadou O, Roussos C, Zakynthinos S
      Thorax, 2007;62(11):950-6.
      Species: Human
      Sample Type: Plasma
    78. Co-occurrence of IgA antibodies against ethanol metabolites and tissue transglutaminase in alcohol consumers: correlation with proinflammatory cytokines and markers of fibrogenesis.
      Authors: Koivisto H, Hietala J, Anttila P, Niemela O
      Dig. Dis. Sci., 2007;53(2):500-5.
      Species: Human
      Sample Type: Serum
    79. Cerebrospinal fluid IL-6, HSP72, and TNF-alpha in exercising humans.
      Authors: Steensberg A, Dalsgaard MK, Secher NH, Pedersen BK
      Brain Behav. Immun., 2006;20(6):585-9.
      Species: Human
      Sample Type: Plasma
    80. The activity of N-acetyl-beta-D-glucosaminidase and tumor necrosis factor-alpha at early stage of diabetic retinopathy development in type 1 diabetes mellitus children.
      Authors: Mysliwiec M, Zorena K, Balcerska A, Mysliwska J, Lipowski P, Raczynska K
      Clin. Biochem., 2006;39(8):851-6.
      Species: Human
      Sample Type: Serum
    81. Postprandial total and HMW adiponectin following a high-fat meal in lean, obese and diabetic men.
      Authors: Phillips L, Peake J, Zhang X, Hickman I, Briskey D, Huang B, Simpson P, Li S, Whitehead J, Martin J, Prins J
      Eur J Clin Nutr, 0;67(4):377-84.
      Species: Human
      Sample Type: Plasma
    Expand to show all 81 Citations
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    Quantikine Control Set 929 for Human HS TNF-a, HSTA00E

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     Human TNF-alpha Quantikine HS ELISA HSTA00D
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      Anonymous 08/04/2017
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     Human TNF-alpha Quantikine HS ELISA HSTA00D
    : Human TNF-alpha Quantikine HS ELISA [HSTA00D]

    Summary

    Sample TestedPeripheral blood neutrophils

    Other Experimental Details

    Other Experimental DetailsDue to the short experimental time, the standard TNFa duoSET ELISA did not work, however HS kit did.
     Human TNF-alpha Quantikine HS ELISA HSTA00D
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      Anonymous 05/25/2016
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     Human TNF-alpha Quantikine HS ELISA HSTA00D
    : Human TNF-alpha Quantikine HS ELISA [HSTA00D]

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    Sample TestedHuman serum

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