Tumor Necrosis Factor (TNF) Receptor-Associated Factors (TRAFs) are a family of adaptor proteins that interact with a wide range of cell surface receptors and participate in the regulation of cell survival, proliferation, differentiation, and stress response. TRAFs were identified by their ability to form complexes with TNF receptor superfamily members but more recently are reported to also bind to Toll/IL‑1 receptor family members and mediate cellular signaling. Six members of the TRAF family have been identified. All TRAF proteins have a homologous C‑terminal TRAF domain that can bind the cytoplasmic domain of receptors as well as other TRAFs. TRAFs 2‑6 have N‑terminal RING and zinc finger domains that are involved in signaling downstream events. Two isoforms of TRAF-3 have been identified, a 368 amino acid, 64 kDa protein (isoform 1, also known as CD40BP and CRAF1) and a 543 amino acid, 61 kDa protein (isoform 2, also known as CAP1 and LAP1). TRAF‑3 has been shown bind specifically to the PEQET sequence motif on the cytoplasmic domain of CD40. TRAF3 and TRAF6 have also been identified as being involved in TLR signaling events.
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
E. coli-derived recombinant human TRAF-3
Met1-Pro543
Accession # EAW81797
Met1-Pro543
Accession # EAW81797
Specificity
Detects human TRAF-3 Isoform 2 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human TRAF-1, -2, -4, -5, or -6 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human TRAF‑3 Isoform 2 Antibody
Detection of Human TRAF‑3 Isoform 2 by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human TRAF-3 Isoform 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3278) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human TRAF-1, TRAF-2, TRAF-3, TRAF-4, TRAF-5, and TRAF-6 (2 ng/lane) were included. A specific band for TRAF-3 was detected at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.Applications for Human TRAF‑3 Isoform 2 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line and Raji human Burkitt's lymphoma cell line
Sample: Jurkat human acute T cell leukemia cell line and Raji human Burkitt's lymphoma cell line
Reviewed Applications
Read 1 review rated 4 using AF3278 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: TRAF-3
Long Name
TNF Receptor-Associated Factor 3
Alternate Names
CAP1, CD40bp, CRAF1, LAP1, TRAF3
Gene Symbol
TRAF3
UniProt
Additional TRAF-3 Products
Product Documents for Human TRAF‑3 Isoform 2 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human TRAF‑3 Isoform 2 Antibody
For research use only
Customer Reviews for Human TRAF‑3 Isoform 2 Antibody (1)
4 out of 5
1 Customer Rating
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
Toll-Like Receptor Signaling Pathways
