Human TRAF-3 Isoform 2 Antibody

Catalog # Availability Size / Price Qty

Save Up to 40% on RUO Reagents with BIOSPRING24 (See Details)

Detection of Human TRAF‑3 Isoform 2 by Western Blot.
1 Image
Product Details
Supplemental Products
Reviews (1)

Human TRAF-3 Isoform 2 Antibody Summary

Species Reactivity
Detects human TRAF-3 Isoform 2 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human TRAF-1, -2, -4, -5, or -6 is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant human TRAF-3
Accession # EAW81797
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human TRAF‑3 Isoform 2 antibody by Western Blot. View Larger

Detection of Human TRAF‑3 Isoform 2 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human TRAF-3 Isoform 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3278) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human TRAF-1, TRAF-2, TRAF-3, TRAF-4, TRAF-5, and TRAF-6 (2 ng/lane) were included. A specific band for TRAF-3 was detected at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TRAF-3

Tumor Necrosis Factor (TNF) Receptor-Associated Factors (TRAFs) are a family of adaptor proteins that interact with a wide range of cell surface receptors and participate in the regulation of cell survival, proliferation, differentiation, and stress response. TRAFs were identified by their ability to form complexes with TNF receptor superfamily members but more recently are reported to also bind to Toll/IL‑1 receptor family members and mediate cellular signaling. Six members of the TRAF family have been identified. All TRAF proteins have a homologous C‑terminal TRAF domain that can bind the cytoplasmic domain of receptors as well as other TRAFs. TRAFs 2‑6 have N‑terminal RING and zinc finger domains that are involved in signaling downstream events. Two isoforms of TRAF-3 have been identified, a 368 amino acid, 64 kDa protein (isoform 1, also known as CD40BP and CRAF1) and a 543 amino acid, 61 kDa protein (isoform 2, also known as CAP1 and LAP1). TRAF‑3 has been shown bind specifically to the PEQET sequence motif on the cytoplasmic domain of CD40. TRAF3 and TRAF6 have also been identified as being involved in TLR signaling events.

Long Name
TNF Receptor-Associated Factor 3
Entrez Gene IDs
7187 (Human); 22031 (Mouse)
Alternate Names
CAP1; CAP-1CD40 receptor associated factor 1; CD40 associated protein 1; CD40 binding protein; CD40 receptor-associated factor 1; CD40-binding protein; CD40bp; CRAF1; CRAF1CAP1; LAP1; LAP1CD40BP; LMP1-associated protein 1; TNF receptor-associated factor 3; TRAF3; TRAF-3

Product Datasheets

You must select a language.



No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human TRAF-3 Isoform 2 Antibody

Average Rating: 4 (Based on 1 Review)

5 Star
4 Star
3 Star
2 Star
1 Star

Have you used Human TRAF-3 Isoform 2 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:

Human TRAF-3 Isoform 2 Antibody
By Christian Ostalecki on 06/07/2018
Application: IHC Sample Tested: Brain tissue Species: Human