Human TRAILR1/TNFRSF10A Antibody
Human TRAILR1/TNFRSF10A Antibody Summary
Ala24-Asn239
Accession # AAC51226
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
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TRAILR1/TNFRSF10A in Human Tonsil Tissue. TRAILR1/TNFRSF10A was detected in immersion fixed paraffin-embedded sections of human tonsil tissue using Mouse Anti-Human TRAILR1/TNFRSF10A Monoclonal Antibody (Catalog # MAB347) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface on lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TRAILR1/TNFRSF10A
Human TRAIL R1, also called DR4, is a type 1, TNF R family, membrane protein which is a receptor for TRAIL (APO2 ligand). In the TNF superfamily nomenclature, TRAIL RI is referred to as TNFRSF10A. TRAIL R1 cDNA encodes a 468 amino acid precursor protein containing extracellular cysteine-rich domains, a transmembrane domain and a cytoplasmic death domain. Among the TNF receptor family proteins, TRAIL R1 is most closely related to TRAIL R2/DR5, sharing 55% amino acid sequence identity. Binding of trimeric TRAIL to TRAIL R1 induces apoptosis. The induction of apoptosis likely requires oligomerization of the receptor. The human TRAIL R1/Fc chimera neutralizes the ability of TRAIL to induce apoptosis. Besides TRAIL R1, an additional TRAIL R2/DR5, which tranduces apoptosis signal, and two TRAIL decoy receptors, which antagonize TRAIL-induced apoptosis, have been reported.
Product Datasheets
Citations for Human TRAILR1/TNFRSF10A Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Marizomib sensitizes primary glioma cells to apoptosis induced by a latest-generation TRAIL receptor agonist
Authors: C Boccellato, E Kolbe, N Peters, V Juric, G Fullstone, M Verreault, A Idbaih, MLM Lamfers, BM Murphy, M Rehm
Cell Death & Disease, 2021;12(7):647.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
CD154 inhibits death of T cells via a Cis interaction with the alpha5beta1 integrin
Authors: M Bachsais, S Salti, K Zaoui, GS Hassan, F Aoudjit, W Mourad
PLoS ONE, 2020;15(8):e0235753.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Stress-induced TRAILR2 expression overcomes TRAIL resistance in cancer cell spheroids
Authors: D Stöhr, JO Schmid, TB Beigl, A Mack, DS Maichl, K Cao, B Budai, G Fullstone, RE Kontermann, TE Mürdter, SWG Tait, C Hagenloche, N Pollak, P Scheurich, M Rehm
Cell Death Differ., 2020;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Bcl-2-mediated control of TRAIL-induced apoptotic response in the non-small lung cancer cell line NCI-H460 is effective at late caspase processing steps
Authors: L Danish, D Imig, F Allgöwer, P Scheurich, N Pollak
PLoS ONE, 2018;13(6):e0198203.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Targeting Apoptotic Activity Against Prostate Cancer Stem Cells
Authors: D Jaworska, E Szliszka
Int J Mol Sci, 2017;18(8):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Caspase-8 expression is predictive of tumour response to death receptor 5 agonist antibody in Ewing's sarcoma.
Authors: Kang Z, Goldstein S, Yu Y, Meltzer P, Loeb D, Cao L
Br J Cancer, 2015;113(6):894-901.
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay -
TRAIL-engineered bone marrow-derived mesenchymal stem cells: TRAIL expression and cytotoxic effects on C6 glioma cells.
Authors: Tang X, Lu J, Tu H, Huang K, Fu R, Cao G, Huang M, Cheng L, Dai L, Zhang L
Anticancer Res, 2014;34(2):729-34.
Species: Rat
Sample Types: Whole Cells
Applications: Flow Cytometry -
Crosstalk between mitochondrial ROS and depolarization in the potentiation of TRAIL-induced apoptosis in human tumor cells.
Authors: Suzuki-Karasaki M, Ochiai T, Suzuki-Karasaki Y
Int J Oncol, 2014;44(2):616-28.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Dominant negative effects of tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) receptor 4 on TRAIL receptor 1 signaling by formation of heteromeric complexes.
Authors: Neumann S, Hasenauer J, Pollak N, Scheurich P
J Biol Chem, 2014;289(23):16576-87.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Exosomes secreted by human placenta carry functional Fas ligand and TRAIL molecules and convey apoptosis in activated immune cells, suggesting exosome-mediated immune privilege of the fetus.
Authors: Stenqvist A, Nagaeva O, Baranov V, Mincheva-Nilsson L
J Immunol, 2013;191(11):5515-23.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Casticin potentiates TRAIL-induced apoptosis of gastric cancer cells through endoplasmic reticulum stress.
Authors: Zhou Y, Tian L, Long L, Quan M, Liu F, Cao J
PLoS ONE, 2013;8(3):e58855.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
The transmembrane domains of TNF-related apoptosis-inducing ligand (TRAIL) receptors 1 and 2 co-regulate apoptotic signaling capacity.
Authors: Neumann S, Bidon T, Branschadel M, Krippner-Heidenreich A, Scheurich P, Doszczak M
PLoS ONE, 2012;7(8):e42526.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
TRAIL-engineered pancreas-derived mesenchymal stem cells: characterization and cytotoxic effects on pancreatic cancer cells.
Cancer Gene Ther., 2012;19(9):652-8.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Drozitumab, a human antibody to death receptor 5, has potent antitumor activity against rhabdomyosarcoma with the expression of caspase-8 predictive of response.
Authors: Kang Z, Chen J, Yu Y, Li B, Sun S, Zhang B, Cao L
Clin Cancer Res, 2011;17(10):3181-92.
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay -
Dexamethasone counteracts the anti-osteoclastic, but not the anti-leukemic, activity of TNF-related apoptosis inducing ligand (TRAIL).
Authors: Zauli G, Rimondi E, Celeghini C, Milani D, Secchiero P
J. Cell. Physiol., 2010;222(2):357-64.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Plasmacytoid dendritic cells express TRAIL and induce CD4+ T-cell apoptosis in HIV-1 viremic patients.
Authors: Stary G, Klein I, Kohlhofer S, Koszik F, Scherzer T, Mullauer L, Quendler H, Kohrgruber N, Stingl G
Blood, 2009;114(18):3854-63.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Differential responses of FLIPLong and FLIPShort-overexpressing human myeloid leukemia cells to TNF-alpha and TRAIL-initiated apoptotic signals.
Authors: Seal S, Hockenbery DM, Spaulding EY, Kiem HP, Abbassi N, Deeg HJ
Exp. Hematol., 2008;36(12):1660-72.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Diallyl trisulfide increases the effectiveness of TRAIL and inhibits prostate cancer growth in an orthotopic model: molecular mechanisms.
Authors: Shankar S, Chen Q, Ganapathy S, Singh KP, Srivastava RK
Mol. Cancer Ther., 2008;7(8):2328-38.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Differential effects of chemotherapeutic drugs versus the MDM-2 antagonist nutlin-3 on cell cycle progression and induction of apoptosis in SKW6.4 lymphoblastoid B-cells.
Authors: Barbarotto E, Corallini F, Rimondi E, Fadda R, Mischiati C, Grill V, Vaccarezza M, Celeghini C
J. Cell. Biochem., 2008;104(2):595-605.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
A novel p53 rescue compound induces p53-dependent growth arrest and sensitises glioma cells to Apo2L/TRAIL-induced apoptosis.
Authors: Weinmann L, Wischhusen J, Demma MJ, Naumann U, Roth P, Dasmahapatra B, Weller M
Cell Death Differ., 2008;15(4):718-729.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
P53-mediated upregulation of DcR1 impairs oxaliplatin/TRAIL-induced synergistic anti-tumour potential in colon cancer cells.
Authors: Toscano F, Fajoui ZE, Gay F, Lalaoui N, Parmentier B, Chayvialle JA, Scoazec JY, Micheau O, Abello J, Saurin JC
Oncogene, 2008;27(30):4161-71.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Cytokines induced during chronic hepatitis B virus infection promote a pathway for NK cell-mediated liver damage.
Authors: Dunn C, Brunetto M, Reynolds G, Christophides T, Kennedy PT, Lampertico P, Das A, Lopes AR, Borrow P, Williams K, Humphreys E, Afford S, Adams DH, Bertoletti A, Maini MK
J. Exp. Med., 2007;204(3):667-80.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
HDAC inhibitors enhance the apoptosis-inducing potential of TRAIL in breast carcinoma.
Authors: Singh TR, Shankar S, Srivastava RK
Oncogene, 2005;24(29):4609-23.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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Total cell lysates from HT29 and MCF-7 were subjected to western blot. PVDF membrane were probed with 1 um/ml Human DR4 Antibody (MAB347). A specific band was detected for DR4 at approximately 55 kDa. This experiment was conducted under reducing conditions.
