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Human TRIM21 Antibody

Catalog #: MAB6219 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB6219
MAB6219-SP

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TRIM21 in Human PBMCs.
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Human TRIM21 Antibody Summary

Species Reactivity
Human
Specificity
Detects human TRIM21 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) TRIM, rhTRIM5, rhTRIM5a, rhTRIM32, rhTRIF (aa 29-204), or rhTRIF (aa 474-618) is observed
Source
Monoclonal Mouse IgG1 Clone # 672722
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human TRIM21
Arg195-Pro293
Accession # P19474
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunoprecipitation
2 µg/mL
A549 human lung carcinoma cell line
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry TRIM21 antibody in Human PBMCs by Immunocytochemistry (ICC). View Larger

TRIM21 in Human PBMCs. TRIM21 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated for 8 hours with 20 ng/mL of Recombinant Human IFN-gamma (285-IF) using Mouse Anti-Human TRIM21 Monoclonal Antibody (Catalog # MAB6219) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; NL009) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunoprecipitation Detection of TRIM21 by Immunoprecipitation. View Larger

Detection of TRIM21 by Immunoprecipitation. A549 human lung carcinoma cell line lysates were prepared and immunoprecipitation was performed using 2.0 μg of Mouse Anti-Human TRIM21 Monoclonal Antibody (Catalog # MAB6219) pre-coupled to Dynabeads Protein G. Immunoprecipitated TRIM21 was detected in Western Blot with a rabbit TRIM21 antibody used at 1/2000. The Ponceau stained transfer of the blot is shown. SM=4% starting material; UB=4% unbound fraction; IP=immunoprecipitate; HC=antibody heavy chain. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TRIM21

TRIM21 (Tripartite motif-containing protein 21; also Ro(SS-A), 52 kDa Ro Protein/Ro52, and RING finger protein 81) is a 52-56 kDa member of the RING finger-B-box-coiled-coil family of proteins. It is an E3 ligase that is found in both nucleus and cytoplasm, where it is often associated with microtubules. TRIM21 ubiquitinates select proteins. In B cells, it targets the Fc fragment of misfolded IgG, providing QC on its production. In macrophages, it acts in a nondegradative manner on IRF8, promoting innate immunity. Human TRIM21 is 475 amino acids (aa) in length. It contains one E3 ligase RING finger domain (aa 16-55), a B-Box type zinc-finger region (aa 92-123), a coiled-coil region (aa 128-238) and a C-terminal SPRY/B30.2 Ig binding domain (aa 268-465). TRIM21 is reported to form trimers. Over aa 195‑293, human TRIM21 exhibits 72% aa identity with mouse TRIM21.

Long Name
Tripartite Motif-containing Protein 21
Entrez Gene IDs
6737 (Human); 20821 (Mouse); 308901 (Rat)
Alternate Names
52 kDa ribonucleoprotein autoantigen Ro/SS-A; 52 kDa Ro/SSA; E3 ubiquitin-protein ligase TRIM21; EC 6.3.2.-; rhTRIM21; RNF81; RNF81RING finger protein 81; ro(SS-A); Ro52; RO52SS-A; Sicca syndrome antigen A; Sjoegren syndrome type A antigen; Sjogren syndrome antigen A1 (52kDa, ribonucleoprotein autoantigen SS-A/Ro); SSA1; SSA1SSA; TRIM21; tripartite motif containing 21,52 kDa Ro protein; tripartite motif-containing 21; Tripartite motif-containing protein 21

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FAQs

  1. What is the light chain of Human TRIM21 Antibody, Catalog # MAB6219?

    • The light chain is kappa.

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