Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 254008
View all Tripeptidyl-Peptidase I/TPP1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Tripeptidyl‑Peptidase I/TPP1
Ser20-Pro563
Accession # O14773

Specificity

Detects human Tripeptidyl‑Peptidase I/TPP1 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for Human Tripeptidyl‑Peptidase I/TPP1 Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Tripeptidyl-Peptidase I/TPP1, see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Recombinant Human Tripeptidyl-Peptidase I/CLN2

Reviewed Applications

Read 1 review rated 4 using MAB2237 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Tripeptidyl-Peptidase I/TPP1

Tripeptidyl-Peptidase I (TPP1), also known as tripeptidyl aminopeptidase, is a lysosomal peptidase which can hydrolyze tripeptides from the N-termini of oligopeptides and also possesses weak endopeptidase activity (1‑3). TPP1 is a serine peptidase with a Ser-Glu-Asp catalytic triad, making it a member of the sedolisin family (4). The TPP1 precursor undergoes autoactivation under conditions of acidic pH (4). TPP1 is expressed in many tissues, with elevated expression in tissues associated with peptide hormone production (5). Mutations in TPP1 have been shown to be a cause of classic late‑infantile neuronal ceroid lipofuscinosis (CLN2), a lysosomal storage disease (6).

References

  1. Sleat, D.E. et al. (1997) Science 277:1802.
  2. Ezaki, J. et al. (1999) J. Neurochem. 72:2573.
  3. Tomkinson, B. (1999) Trends Biochem. Sci. 24:355.
  4. Lin, L. et al. (2001) J. Biol. Chem. 276:2249.
  5. Kida, E. et al. (2001) J. Neuropathol. Exp. Neurol. 60:280.
  6. Sleat, D.E. et al. (1999) Am. J. Hum. Genet. 64:1511.

Alternate Names

CLN2, LINCL, LPIC, TPP1, TripeptidylPeptidase I

Entrez Gene IDs

1200 (Human)

Gene Symbol

TPP1

UniProt

O14773

Additional Tripeptidyl-Peptidase I/TPP1 Products

Product Documents for Human Tripeptidyl‑Peptidase I/TPP1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Tripeptidyl‑Peptidase I/TPP1 Antibody

For research use only

Related Research Areas

Serine Proteases and Regulators

Citations for Human Tripeptidyl‑Peptidase I/TPP1 Antibody

Customer Reviews for Human Tripeptidyl‑Peptidase I/TPP1 Antibody (1)

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  • Name: Anonymous
    Application: Functional Assay
    Sample Tested: Trigeminal ganglia
    Species: Mouse
    Verified Customer | Posted 06/04/2019

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