Human Tripeptidyl-Peptidase I/TPP1 Antibody

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Human Tripeptidyl-Peptidase I/TPP1 Antibody Summary

Species Reactivity
Detects human Tripeptidyl‑Peptidase I/TPP1 in direct ELISAs and Western blots.
Monoclonal Mouse IgG2A Clone # 254008
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant human Tripeptidyl‑Peptidase I/TPP1
Accession # O14773
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

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Recommended Concentration
Western Blot
1 µg/mL
Recombinant Human Tripeptidyl-Peptidase I/CLN2
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Tripeptidyl-Peptidase I/TPP1, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Tripeptidyl-Peptidase I/TPP1

Tripeptidyl-Peptidase I (TPP1), also known as tripeptidyl aminopeptidase, is a lysosomal peptidase which can hydrolyze tripeptides from the N-termini of oligopeptides and also possesses weak endopeptidase activity (1‑3). TPP1 is a serine peptidase with a Ser-Glu-Asp catalytic triad, making it a member of the sedolisin family (4). The TPP1 precursor undergoes autoactivation under conditions of acidic pH (4). TPP1 is expressed in many tissues, with elevated expression in tissues associated with peptide hormone production (5). Mutations in TPP1 have been shown to be a cause of classic late‑infantile neuronal ceroid lipofuscinosis (CLN2), a lysosomal storage disease (6).

  1. Sleat, D.E. et al. (1997) Science 277:1802.
  2. Ezaki, J. et al. (1999) J. Neurochem. 72:2573.
  3. Tomkinson, B. (1999) Trends Biochem. Sci. 24:355.
  4. Lin, L. et al. (2001) J. Biol. Chem. 276:2249.
  5. Kida, E. et al. (2001) J. Neuropathol. Exp. Neurol. 60:280.
  6. Sleat, D.E. et al. (1999) Am. J. Hum. Genet. 64:1511.
Entrez Gene IDs
1200 (Human)
Alternate Names
Cell growth-inhibiting gene 1 protein; ceroid-lipofuscinosis, neuronal 2, late infantile (Jansky-Bielschowsky disease); CLN2; CLN2EC; growth-inhibiting protein 1; LINCL; LPIC; lysosomal pepstatin insensitive protease; Lysosomal pepstatin-insensitive protease; MGC21297; TPP1; TPP-1; TPP-I; Tripeptidyl aminopeptidase; tripeptidyl peptidase I; tripeptidyl-peptidase 1; TripeptidylPeptidase I; Tripeptidyl-Peptidase I

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Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

Reviews for Human Tripeptidyl-Peptidase I/TPP1 Antibody

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By Anonymous on 06/04/2019
Application: Func Sample Tested: Trigeminal ganglia Species: Mouse