Detection of TSPAN2 in NS0 Mouse Cell Line Co-transfected with TSPAN2 and eGFP by Flow Cytometry.
NS0 mouse myeloma cell line co-transfected with human TSPAN2 and eGFP was stained with either (A) Mouse IgG2B Isotype Control (Catalog # MAB0041) or (B) Mouse Anti-Human TSPAN2 Monoclonal Antibody (Catalog # MAB7876) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
TSPAN2 (tetraspanin-2) is a member of the transmembrane 4 superfamily. It is a cell surface 221 amino acid (aa) protein with 4 transmembrane segments and a large extracellular loop (aa 112‑188) that contains four conserved cysteines and one potential N-glycosylation site. Both the N- and C-termini are intracellular. In humans, TSPAN2 expressed sequence tags have been found in the pregnant uterus, T cell, and fetal heart. In rats, TSPAN2 is thought to be involved in oligodendrocyte differentiation. Human TSPAN2 shares 94% and 95% aa sequence identity with mouse and rat TSPAN2, respectively.
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