Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 590409
View all TSPAN9 Primary Antibodies »

Product Specifications

Immunogen

HEK293 human embryonic kidney cell line transfected with human TSPAN9
Accession # O75954

Specificity

Detects human TSPAN9 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human TSPAN9 Antibody

Detection of Human TSPAN9 antibody by Western Blot.

Detection of Human TSPAN9 by Western Blot.

Western blot shows lysates of human kidney tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human TSPAN9 Monoclonal Antibody (Catalog # MAB6565) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for TSPAN9 at approximately 27 to 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human TSPAN9 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Human kidney tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TSPAN9

Tetraspanin 9 (TSPAN9) is a 27 kDa glycoprotein and member of the tetraspanin (TM4SF) family of proteins. It is 239 amino acids (aa) in length and contains four transmembrane segments and one potential site for N-linked glycosylation. Both the N- and C-termini lie on the intracellular side of the membrane. Human TSPAN9 is 97% aa identical to mouse TSPAN9. It is expressed in megakaryocytes and platelets where it co-localizes with GP6 in tetraspanin microdomains on the platelet surface. TSPAN9 is involved in platelet activation.

Long Name

Tetraspanin 9

Alternate Names

NET5

Entrez Gene IDs

10867 (Human)

Gene Symbol

TSPAN9

UniProt

O75954

Additional TSPAN9 Products

Product Documents for Human TSPAN9 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human TSPAN9 Antibody

For research use only

Related Research Areas

Integrins and Associated Molecules

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