Human ULBP-3 Antibody (166514)

R&D Systems | Catalog # MAB15171

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Flow Cytometry, ELISA Detection, ELISA Development

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 166514
View all ULBP-3 Primary Antibodies »

Product Specifications

Immunogen

BaF3 mouse pro-B cell line transfected with human ULBP-3
Accession # Q9BZM4

Specificity

Detects human ULBP-3 in direct ELISAs and Western blots. In these formats, this antibody does not cross‑react with recombinant human (rh) ULBP-1 or rhULBP-2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human ULBP-3 Antibody (166514)

Detection of ULBP-3 by Flow Cytometry

Detection of ULBP-3 by Flow Cytometry

Viral U20 and U21 proteins localize to the secretory pathway. (A) Localization of U20, U21 and U23 in 293T cells determined by FLAG tag staining (yellow). PDI was co-stained to assess the localization to the endoplasmic reticulum (red, left); CD107a served as a marker for late endosomes (red, right). Nuclei were counterstained with DAPI (blue). Pearson’s correlation coefficients describe overlap of FLAG staining and organelle marker staining. (B) Flow cytometric analysis of ULBP3 on J-Jhan and 293T transfected with U21 or controls treated for 6 hours with Concanamycin A that inhibits lysosomal degradation of proteins. A representative plot is presented for ULBP3 expression on J-Jhan transfectants. (C) Bar histograms show quantification of one of two independent experiments, *p < 0.05 in student’s t test for U21-transfectants in J-Jhan and 293T cells. Differences in all other transfectants are non-significant. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34721381), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human ULBP-3 Antibody (166514)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human ULBP‑3 Fc Chimera (Catalog # 1517-UL)

Reviewed Applications

Read 1 review rated 5 using MAB15171 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ULBP-3

ULBP-3 is a GPI-linked cell-surface protein that functions as a ligand for human NKG2D. ULBP-1, -2, and -3 are expressed on some tumor cells and have been implicated in immune surveillance of tumors. Unlike ULBP-1 and -2, ULBP-3 does not interact with the CMV glycoprotein UL16.

Long Name

UL16 Binding Protein-3

Alternate Names

ULBP3

Entrez Gene IDs

79465 (Human)

Gene Symbol

ULBP3

UniProt

Q9BZM4

Additional ULBP-3 Products

Product Documents for Human ULBP-3 Antibody (166514)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human ULBP-3 Antibody (166514)

For research use only

Citations for Human ULBP-3 Antibody (166514)

Customer Reviews for Human ULBP-3 Antibody (166514) (1)

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  • Human ULBP-3 Antibody
    Name: Marry Tahani
    Application: Block/Neutralize
    Sample Tested: Pancreatic cancer cells
    Species: Mouse
    Verified Customer | Posted 11/16/2018

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