Human VAMP-8 Antibody

Catalog # Availability Size / Price Qty
Human VAMP-8 Antibody in Data
2 Images
Product Details
Citations (3)
Supplemental Products

Human VAMP-8 Antibody Summary

Species Reactivity
Detects human VAMP-8 in direct ELISAs and Western blots.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant human VAMP-8
Accession # Q9BV40
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below
1-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human VAMP‑8 antibody by Western Blot. View Larger

Detection of Human VAMP‑8 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human VAMP-8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5354) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for VAMP-8 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunohistochemistry VAMP-8 antibody in Human Pancreas by Immunohistochemistry (IHC-P). View Larger

VAMP‑8 in Human Pancreas. VAMP-8 was detected in immersion fixed paraffin-embedded sections of human pancreas using Goat Anti-Human VAMP-8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5354) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to exocrine cell cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VAMP-8

VAMP-8 (vesicle associated membrane protein 8; also endobrevin) is a 12 kDa member of the synaptobrevin family of proteins. It is a type IV transmembrane (TM) protein (i.e.- a type II TM protein whose C-terminus is predominately transmembrane) that is found in cells involved in vesicle release (platelets, mast cells and pancreatic acinar cells). It regulates exocytosis and decouples granule release from cytokine release. Human VAMP-8 is 100 amino acids (aa) in length. It contains a vSNARE coiled-coil homology region (aa 12-72) and a membrane-anchor domain (aa 76‑96). Over aa 1‑75, human VAMP-8 shares 93% aa identity with mouse

Long Name
Vesicle-Associated Membrane Protein 8
Entrez Gene IDs
8673 (Human); 22320 (Mouse); 83730 (Rat)
Alternate Names
EDB; EDBVAMP-8; Endobrevin; VAMP8; VAMP-8; vesicle-associated membrane protein 8 (endobrevin); vesicle-associated membrane protein 8

Product Datasheets

Citations for Human VAMP-8 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
Filter your results:

Filter by:

  1. Munc13-4 functions as a Ca2+ sensor for homotypic secretory granule fusion to generate endosomal exocytic vacuoles.
    Authors: Sang Su Woo, Declan J James, Thomas F J Martin
    Molecular Biology of the Cell, 2017;0(0):1939-4586.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. APOL1 variants change C-terminal conformational dynamics and binding to SNARE protein VAMP8
    Authors: SM Madhavan, JF O'Toole, M Konieczkow, L Barisoni, DB Thomas, S Ganesan, LA Bruggeman, M Buck, JR Sedor
    JCI Insight, 2017;2(14):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  3. SNAP23 Regulates Endothelial Exocytosis of von Willebrand Factor.
    Authors: Zhu Q, Yamakuchi M, Lowenstein C
    PLoS ONE, 2015;10(8):e0118737.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot


No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human VAMP-8 Antibody

There are currently no reviews for this product. Be the first to review Human VAMP-8 Antibody and earn rewards!

Have you used Human VAMP-8 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review