Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Adhesion Blockade, Simple Western

Cited:

Western Blot, Neutralization, Flow Cytometry, Immunocytochemistry, Bioassay, ELISA Development

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all VCAM-1/CD106 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line (CHO)-derived recombinant human VCAM-1
Extracellular domain

Specificity

Detects VCAM-1 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant mouse VCAM-1 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human VCAM‑1/CD106 Antibody

Detection of Human VCAM-1/CD106 antibody by Western Blot.

Detection of Human VCAM‑1/CD106 by Western Blot.

Western blot shows lysates of 786-O human renal cell adenocarcinoma cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human VCAM-1/ CD106 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF809) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for VCAM-1/CD106 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human VCAM‑1/CD106 by Simple WesternTM.

Simple Western lane view shows lysates of HUVEC human umbilical vein endothelial cells untreated (-) or treated (+) with 10 ng/ml Recombinant Human TNF‑ alpha (210-TA) for 24 hrs, loaded at 0.2 mg/mL. A specific band was detected for VCAM‑1/CD106 at approximately 132 kDa (as indicated) using 20 µg/mL of Sheep Anti-Human VCAM‑1/CD106 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF809) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of Mouse VCAM-1/CD106 by Flow Cytometry

Detection of Mouse VCAM-1/CD106 by Flow Cytometry

Inhibition of ICAM-1- and VCAM-1-mediated cell adhesion reduces T-ALL burden in vivo. A Experimental schematic depicting dosing schedule for anti-ICAM-1, anti-VCAM-1, or isotype control antibodies following establishment of LN3 T-ALL ( > 1% spleen chimerism) in congenic hosts. Leukemia burden was assessed 2 days after the final antibody injection. b Quantification of spleen and liver weights from the experiments depicted in (a). Tumor-free, age-matched mice were included for comparison. Bars depict the mean + SEM of cumulative data from n = 5 experiments, each with a distinct color-coded primary T-ALL; symbols represent individual mice. c Representative flow cytometry plots showing a decrease in T-ALL burden in the spleens of LN3 T-ALL-transplanted mice following treatment with anti-ICAM-1 +/- anti-VCAM-1 antibodies (100 µg each per mouse). d, e Quantification of the (d) frequency of circulating T-ALL blasts in the blood and (e) number of T-ALL cells in the spleen, BM, LN, and liver from the same experiments as in (b). f Quantification of the indicated myeloid subsets or TCR beta + or B220+ host cells in the spleens of mice from the same experiments as in (d, e). Statistical significance was determined by (b, d, e, f) a two-sided repeated measures one-way ANOVA with the Holm-Sidak correction; P-values: *<0.05, **<0.01, ***<0.001. ns, not significant. Source data are provided as a Source Data file. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37805579), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human VCAM‑1/CD106 Antibody

Application
Recommended Usage

Adhesion Blockade

The adhesion of U937 human histiocytic lymphoma cells (5 x 104 cells/well) to immobilized Recombinant Human VCAM-1/CD106 (Catalog # ADP5, 2.5 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 25 µg/mL of the antibody.

Simple Western

20 µg/mL
Sample: HUVEC human umbilical vein endothelial cells

Western Blot

2 µg/mL
Sample: 786‑O human renal cell adenocarcinoma cell line

Reviewed Applications

Read 3 reviews rated 4.3 using AF809 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: VCAM-1/CD106

VCAM-1 (CD106), a member of the immunoglobulin superfamily, is a cell surface protein expressed by activated endothelial cells and certain leukocytes (such as macrophages). VCAM-1 expression is induced by IL-1 beta, IL-4, TNF-alpha, and IFN-gamma. VCAM-1 binds to leukocyte integrins VLA-4 and alpha 4 beta 7. The human and mouse VCAM-1 proteins share approximately 76% amino acid similarity.

During the inflammatory adhesion mechanism, activated integrins halt rolling leukocytes and attach them firmly to the vascular endothelium. They do this by binding to their ligands, for example VCAM-1, on endothelium. The VCAM-1: VLA-4/ alpha 4 beta 7 interaction is also thought to be involved in the extravasation of white blood cells through the blood vessel wall to sites of inflammation.

ELISA techniques have shown that detectable levels of soluble VCAM-1 are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of VCAM-1 may be elevated or lowered in subjects with a variety of pathological conditions.

Long Name

Vascular Cell Adhesion Molecule 1

Alternate Names

CD106, VCAM1

Entrez Gene IDs

7412 (Human); 22329 (Mouse)

Gene Symbol

VCAM1

Additional VCAM-1/CD106 Products

Product Documents for Human VCAM‑1/CD106 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human VCAM‑1/CD106 Antibody

For research use only

Citations for Human VCAM‑1/CD106 Antibody

Customer Reviews for Human VCAM‑1/CD106 Antibody (3)

4.3 out of 5
3 Customer Ratings
5 Stars
33%
4 Stars
67%
3 Stars
0%
2 Stars
0%
1 Stars
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Showing  1 - 3 of 3 reviews Showing All
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  • Human VCAM-1/CD106 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 07/23/2019
    Human VCAM‑1/CD106 Antibody AF809
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Purified protein
    Species: Mouse
    Verified Customer | Posted 03/23/2018
  • Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 11/03/2017
    The VCAM antibody (AF809) was used as the capture antibody in a competitive ELISA assay. It was absorbed onto an ELISA plate. The competitor was recombinant VCAM (809-VR) that had been biotinylated. The calibrator curve also used recombinant VCAM (809-VR). Normal human serum and plasma samples were all quantifiable.
    Human VCAM‑1/CD106 Antibody AF809

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