Human VEGF-D Antibody

Catalog # Availability Size / Price Qty
MAB2861-SP
MAB2861-500
MAB2861-100

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Product Details
Citations (3)
FAQs
Supplemental Products
Reviews (4)

Human VEGF-D Antibody Summary

Species Reactivity
Human
Specificity
Detects human VEGF-D in ELISAs and Western blots. In ELISAs, this antibody shows less than 1% cross-reactivity with recombinant human (rh) VEGF121, rhVEGF165, rhVEGF-B167, rhVEGF-B186, and rhVEGF-C. In Western blots, this antibody shows 100% cross-reactivity with rmVEGF‑D, less than 5% cross-reactivity with rhVEGF121, rhVEGF165, rmVEGF120, and no cross-reactivity with rhVEGF-B165, rmVEGF-B186, rhVEGF-B186, rmVEGF115, rrVEGF164 or rhVEGF206.
Source
Monoclonal Mouse IgG1 Clone # 78902
Purification
Protein A or G purified from ascites
Immunogen
Mouse myeloma cell line NS0-derived recombinant human VEGF-D
Phe93-Ser201
Accession # O43915
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human VEGF-D (Catalog # 622-VD)

Human VEGF-D Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human VEGF‑D Biotinylated Antibody (Catalog # BAM286)

Standard: Recombinant Human VEGF-D Protein (Catalog # 622-VD)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VEGF-D

Vascular endothelial growth factor D (VEGF-D), also known as c-fos-induced growth factor (FIGF), is a secreted glycoprotein of the VEGF/PDGF family. VEGFs regulate angiogenesis and lymphangiogenesis during development and tumor growth, and are characterized by eight conserved cysteine residues that form a cystine knot structure (1-3). VEGF-C and VEGF-D, which share 23% amino acid (aa) sequence identity, are uniquely expressed as preproproteins that contain long N- and
C‑terminal propeptide extensions around the VEGF homology domain (VHD) (1, 2). Proteolytic processing of the 354 aa VEGF-D preproprotein creates a secreted proprotein. Further processing by extracellular serine proteases, such as plasmin or furin-like proprotein convertases, forms mature VEGF-D consisting of
non‑covalently linked 42 kDa homodimers of the 117 aa VHD (4-6). Mature human VEGF-D shares 94%, 95%, 99%, 97% and 93% aa identity with mouse, rat, equine, canine and bovine VEGF-D, respectively (4, 5). It is expressed in adult lung, heart, muscle, and small intestine, and is most abundantly expressed in fetal lungs and skin (1-4). Mouse and human VEGF-D are ligands for VEGF Receptor 3 (VEGF R3, also called Flt-4) that are active across species and show enhanced affinity when processed (7). Processed human VEGF-D is also a ligand for VEGF R2, also called Flk-1 or KDR (7). VEGF R3 is strongly expressed in lymphatic endothelial cells and is essential for regulation of the growth and differentiation of lymphatic endothelium (1, 2). While VEGF-C is the critical ligand for VEGF R3 during embryonic lymphatic development, VEGF-D is most active in neonatal lymphatic maturation and bone growth (8-10). Both promote tumor lymphangiogenesis (11). Consonant with their activity on VEGF receptors, binding of VEGF-C and VEGF-D to neuropilins contributes to VEGF R3 signaling in lymphangiogenesis, while binding to integrin alpha 9 beta 1 mediates endothelial cell adhesion and migration (12, 13).

 

References
  1. Roy, H. et al. (2006) FEBS Lett. 580:2879.
  2. Otrock, Z.H. et al. (2007) Blood Cells Mol. Dis. 38:258.
  3. Yamada, Y. et al. (1997) Genomics 42:483.
  4. Stacker, S.A. et al. (1999) J. Biol. Chem. 274:32127.
  5. McColl, B.K. et al. (2003) J. Exp. Med. 198:863.
  6. McColl, B.K. et al. (2007) FASEB J. 21:1088.
  7. Baldwin, M.E. et al. (2001) J. Biol. Chem. 276:19166.
  8. Baldwin, M.E. et al. (2005) Mol. Cell. Biol. 25:2441.
  9. Karpanen, T. et al. (2006) Am. J. Pathol. 169:708.
  10. Orlandini, M. et al. (2006) J. Biol. Chem. 281:17961.
  11. Stacker, S.A. et al. (2001) Nature Med. 7:186.
  12. Karpanen, T. et al. (2006) FASEB J. 20:1462.
  13. Vlahakis, N.E. et al. (2005) J. Biol. Chem. 280:4544.
Long Name
Vascular Endothelial Growth Factor D/cFos-induced Growth Factor
Entrez Gene IDs
2277 (Human); 14205 (Mouse)
Alternate Names
c-fos induced growth factor (vascular endothelial growth factor D); FIGF; vascular endothelial growth factor D; VEGFD; VEGF-D; VEGF-DVEGFDc-Fos-induced growth factor

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Citations for Human VEGF-D Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Pulmonary Vasculopathy Associated with FIGF Gene Mutation
    Authors: Souheil Y El-Chemaly
    Am. J. Pathol., 2016;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Differential receptor binding and regulatory mechanisms for the lymphangiogenic growth factors VEGF-C and VEGF-D
    Authors: Marc G Achen
    J. Biol. Chem., 2016;0(0):.
    Species: Human
    Sample Types: Protein
    Applications: Western Blot
  3. Autocrine effects of VEGF-D on endothelial cells after transduction with AD-VEGF-D(DeltaNDeltaC).
    Authors: Papiewska-Pajak I, Boncela J, Przygodzka P, Cierniewski CS
    Exp. Cell Res., 2010;316(6):907-14.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Reviews for Human VEGF-D Antibody

Average Rating: 3.8 (Based on 4 Reviews)

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Human VEGF-D Antibody
By Anonymous on 06/10/2020
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human VEGF-D Antibody
By Anonymous on 11/10/2018
Application: ELISA Sample Tested: Plasma Species: Human

Human VEGF-D Antibody
By Anonymous on 11/09/2018
Application: MiAr Sample Tested: EDTA Plasma Species: Human

Human VEGF-D Antibody
By Anonymous on 11/07/2018
Application: Microarrays Sample Tested: EDTA Plasma Species: Human