Human VEGFR2/KDR/Flk-1 Antibody

Name: Human VEGFR2/KDR/Flk-1 Antibody
Brand: R&D Systems
SKU: MAB3573
Price: 489 USD
Availability: InStock

R&D Systems | Catalog # MAB3573

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Citations (7)

Product Documents

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Product Specifications
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, ELISA Capture (Matched Antibody Pair)

Cited:

Western Blot, Neutralization, Immunocytochemistry, ELISA Development

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # 89109

View all VEGFR2/KDR/Flk-1 Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human VEGFR2/KDR/Flk-1
Ala20-Glu764
Accession # P35968

Specificity

Detects human VEGFR2/KDR/Flk-1 in ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) VEGFR1, rhVEGFR3, or recombinant mouse VEGFR2 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Applications for Human VEGFR2/KDR/Flk-1 Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human VEGFR2/KDR/Flk-1 Fc Chimera (Catalog # 357-KD)
under non-reducing conditions only. It is recommended to use Goat Anti-Human VEGFR2/KDR/Flk‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF357).

Human VEGFR2/KDR Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)

Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:

Detection Reagent: Human VEGFR2/KDR/Flk‑1 Biotinylated Antibody (Catalog # BAF357)
Standard: Recombinant Human VEGFR2/KDR Fc Chimera Protein (Catalog # 357-KD)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: VEGFR2/KDR/Flk-1

VEGFR2 (KDR/Flk-1), VEGFR1 (Flt-1) and VEGFR3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGFR1, 2, and 3 is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis. VEGFR2 cDNA encodes a 1356 amino acid (aa) residue precursor protein with a 19 aa residue signal peptide. Mature VEGFR2 is composed of a 745 aa residue extracellular domain, a 25 aa residue transmembrane domain and a 567 aa residue cytoplasmic domain. In contrast to VEGFR1 which binds both PlGF and VEGF with high affinity, VEGFR2 binds VEGF but not PlGF with high affinity. The recombinant soluble VEGFR2/Fc chimera binds VEGF with high affinity and is a potent VEGF antagonist.