Human XIAP Antibody

Catalog # Availability Size / Price Qty
MAB8221
MAB8221-SP
Human XIAP Antibody in Immunohistochemistry
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Product Details
Citations (1)
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Human XIAP Antibody Summary

Species Reactivity
Human
Specificity
Detects human XIAP in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) cIAP-1 or rh cIAP-2 is observed. Neutralizes human XIAP and human XIAP-BIR2 bioactivity. Does not neutralize XIAP-BIR3 or Livin bioactivity.
Source
Monoclonal Mouse IgG1 Clone # 117318
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human XIAP
Met1-Ser497
Accession # P98170
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
8-25 µg/mL
See below
Neutralization
Measured by its ability to neutralize Recombinant Human XIAP (2.75 µg/mL, Catalog # 822-XF) inhibition of Recombinant Human Caspase‑7 (0.012 µg/mL, Catalog # 823-C7) cleavage of the fluorogenic peptide substrate DEVD-AFC (50 µM). The Neutralization Dose (ND50) is typically 10 µg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunohistochemistry XIAP in Human Brain. View Larger

XIAP in Human Brain. XIAP was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using 25 µg/mL Mouse Anti-Human XIAP Monoclonal Antibody (Catalog # MAB8221) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Neutralization Neutralization of XIAP Activity by Human XIAP Antibody. View Larger

Neutralization of XIAP Activity by Human XIAP Antibody. Recombinant Human Caspase-7 (0.012 µg/mL, Catalog # 823-C7) activity is measured in the presence of Recombinant Human XIAP (2.75 µg/mL, Catalog # 822-XF) that has been preincubated with increasing concentrations of Mouse Anti-Human XIAP Antibody (Catalog # MAB8221) The ND50is typically 10 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: XIAP

XIAP (X-chromosome linked inhibitor of apoptosis) is a member of the apoptosis (IAP) family of proteins that inhibit caspases. The BIR2 domain of XIAP inhibits caspase-3 and caspase-7. The ability of XIAP to inhibit caspases is prevented by SMAC/Diablo through binding to XIAP-BIR2 and -BIR3 domains.

Long Name
X-linked Inhibitor of Apoptosis
Entrez Gene IDs
331 (Human); 11798 (Mouse); 63879 (Rat)
Alternate Names
API3X-linked inhibitor of apoptosis protein; apoptosis inhibitor 3; baculoviral IAP repeat-containing 4; baculoviral IAP repeat-containing protein 4; BIRC4; BIRC4XLP2; E3 ubiquitin-protein ligase XIAP; EC 6.3.2.-; hIAP3; hIAP-3; hILP; IAP3; IAP-3; IAP-like protein; ILP; ILP1; Inhibitor of apoptosis protein 3; MIHA; XIAP; X-linked IAP; X-linked inhibitor of apoptosis

Product Datasheets

Citation for Human XIAP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. A small peptide modeled after the NRAGE repeat domain inhibits XIAP-TAB1-TAK1 signaling for NF-kappaB activation and apoptosis in P19 cells.
    Authors: Rochira JA, Matluk NN, Adams TL, Karaczyn AA, Oxburgh L, Hess ST, Verdi JM
    PLoS ONE, 2011;6(7):e20659.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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