Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Neutralization

Cited:

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 117318
View all XIAP Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human XIAP
Met1-Ser497
Accession # P98170

Specificity

Detects human XIAP in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) cIAP-1 or rh cIAP-2 is observed. Neutralizes human XIAP and human XIAP-BIR2 bioactivity. Does not neutralize XIAP-BIR3 or Livin bioactivity.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human XIAP Antibody

XIAP antibody in Human Brain by Immunohistochemistry (IHC-P).

XIAP in Human Brain.

XIAP was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using 25 µg/mL Mouse Anti-Human XIAP Monoclonal Antibody (Catalog # MAB8221) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Neutralization of XIAP Activity by Human XIAP Antibody.

Neutralization of XIAP Activity by Human XIAP Antibody.

Recombinant Human Caspase-7 (0.012 µg/mL, Catalog # 823-C7) activity is measured in the presence of Recombinant Human XIAP (2.75 µg/mL, Catalog # 822-XF) that has been preincubated with increasing concentrations of Mouse Anti-Human XIAP Antibody (Catalog # MAB8221) The ND50 is typically 10 µg/mL.

Applications for Human XIAP Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cortex)

Neutralization

Measured by its ability to neutralize Recombinant Human XIAP (2.75 µg/mL, Catalog # 822-XF) inhibition of Recombinant Human Caspase‑7 (0.012 µg/mL, Catalog # 823-C7) cleavage of the fluorogenic peptide substrate DEVD-AFC (50 µM). The Neutralization Dose (ND50) is typically 10 µg/mL.

Reviewed Applications

Read 2 reviews rated 3 using MAB8221 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: XIAP

XIAP (X-chromosome linked inhibitor of apoptosis) is a member of the apoptosis (IAP) family of proteins that inhibit caspases. The BIR2 domain of XIAP inhibits caspase-3 and caspase-7. The ability of XIAP to inhibit caspases is prevented by SMAC/Diablo through binding to XIAP-BIR2 and -BIR3 domains.

Long Name

X-linked Inhibitor of Apoptosis

Alternate Names

BIRC4

Entrez Gene IDs

331 (Human); 11798 (Mouse); 63879 (Rat)

Gene Symbol

XIAP

UniProt

P98170

Additional XIAP Products

Product Documents for Human XIAP Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human XIAP Antibody

For research use only

Related Research Areas

Cysteine Proteases and Regulators

Citations for Human XIAP Antibody

Customer Reviews for Human XIAP Antibody (2)

3 out of 5
2 Customer Ratings
5 Stars
50%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
50%

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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • Human XIAP Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Brain tissue
    Species: Human
    Verified Customer | Posted 04/03/2022
    Brain tissue
    25 ug/mL dilution for ihc
    Human XIAP Antibody MAB8221
  • Human XIAP Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Blood mononuclear cells (PBMCs)
    Species: Human
    Verified Customer | Posted 12/11/2016
    This antibody DOES NOT work with flow cytometry. Intracellularly stained at dilutions of 0, 1:300, 1:60, 1:30, and 1:15. Shifts seen are similar to those expected from background staining.
    Fixed with BD Fix/perm Intracellular stained with BD perm/wash Top graph shows stainings with various dilutions in gated monocytes Bottom graph shows bulk lymphocytes. Grey filled histogram represents no antibody staining.
    Human XIAP Antibody MAB8221

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Protocols

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