Detects human XIAP in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) cIAP-1 or rh cIAP-2 is observed. Neutralizes human XIAP and human XIAP-BIR2 bioactivity. Does not neutralize XIAP-BIR3 or Livin bioactivity.
Monoclonal Mouse IgG1 Clone # 117318
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human XIAP Met1-Ser497 Accession # P98170
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Measured by its ability to neutralize Recombinant Human XIAP (2.75 µg/mL, Catalog # 822-XF) inhibition of Recombinant Human Caspase‑7 (0.012 µg/mL, Catalog # 823-C7) cleavage of the fluorogenic peptide substrate DEVD-AFC (50 µM). The Neutralization Dose (ND50) is typically 10 µg/mL.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
XIAP was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using 25 µg/mL Mouse Anti-Human XIAP Monoclonal Antibody (Catalog # MAB8221) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Neutralization of XIAP Activity by Human XIAP Antibody.
Recombinant Human Caspase-7 (0.012 μg/mL, Catalog # 823-C7) activity is measured in the presence of Recombinant Human XIAP (2.75 μg/mL, Catalog # 822‑XF) that has been preincubated with increasing concentrations of Mouse Anti-Human XIAP Antibody (Catalog # MAB8221) The ND50 is typically 10 μg/mL.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70 °C as supplied.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
XIAP (X-chromosome linked inhibitor of apoptosis) is a member of the
apoptosis (IAP) family of proteins that inhibit caspases. The BIR2
domain of XIAP inhibits caspase-3 and caspase-7. The ability of XIAP to
inhibit caspases is prevented by SMAC/Diablo through binding to
XIAP-BIR2 and -BIR3 domains.
X-linked Inhibitor of Apoptosis
Entrez Gene IDs:
331 (Human); 11798 (Mouse); 63879 (Rat)
API3X-linked inhibitor of apoptosis protein; apoptosis inhibitor 3; baculoviral IAP repeat-containing 4; baculoviral IAP repeat-containing protein 4; BIRC4; BIRC4XLP2; E3 ubiquitin-protein ligase XIAP; EC 6.3.2.-; hIAP3; hIAP-3; hILP; IAP3; IAP-3; IAP-like protein; ILP; ILP1; Inhibitor of apoptosis protein 3; MIHA; XIAP; X-linked IAP; X-linked inhibitor of apoptosis
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