Detection of Human XPV by Western Blot. Western blot shows lysates of MRC-5 human embryonic lung fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Human XPV Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF3417) followed by HRP‑conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for XPV at approximately 80 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 5.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Xeroderma pigmentosum variant (XPV) is a form of XP caused by mutation in the DNA polymerase eta (POLH) gene. DNA polymerase eta catalyzes translesion DNA synthesis past pyrimidine dimers, a mutation caused by exposure to ultraviolet (UV) light. XPV patients are sensitive to UV light and show an increased incidence of skin cancer.
Xeroderma Pigmentosum Variant Type Protein
Entrez Gene IDs:
5429 (Human); 80905 (Mouse); 316235 (Rat)
DNA polymerase eta; DNA Polymerase, eta; EC 18.104.22.168; POLH; polymerase (DNA directed), eta; RAD30 homolog A; RAD30; RAD30A; RAD30AFLJ16395; Xeroderma pigmentosum variant type protein; XP-V; XPVFLJ21978
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