Human XPV Antibody

Catalog # Availability Size / Price Qty
Detection of Human XPV by Western Blot.
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Product Details
Supplemental Products

Human XPV Antibody Summary

Species Reactivity
Detects human XPV in Western blots.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant human XPV
Accession # Q9Y253
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human XPV antibody by Western Blot. View Larger

Detection of Human XPV by Western Blot. Western blot shows lysates of MRC-5 human embryonic lung fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Human XPV Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3417) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for XPV at approximately 80 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 5.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: XPV

Xeroderma pigmentosum variant (XPV) is a form of XP caused by mutation in the DNA polymerase eta (POLH) gene. DNA polymerase eta catalyzes translesion DNA synthesis past pyrimidine dimers, a mutation caused by exposure to ultraviolet (UV) light. XPV patients are sensitive to UV light and show an increased incidence of skin cancer.

Long Name
Xeroderma Pigmentosum Variant Type Protein
Entrez Gene IDs
5429 (Human); 80905 (Mouse); 316235 (Rat)
Alternate Names
DNA polymerase eta; DNA Polymerase, eta; EC; POLH; polymerase (DNA directed), eta; RAD30 homolog A; RAD30; RAD30A; RAD30AFLJ16395; Xeroderma pigmentosum variant type protein; XPV; XP-V; XPVFLJ21978

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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