Q: I was wondering if you know of any blocking antibodies against IL-6 or VEGF-C that can be used in cell culture to quench these cytokines?

A: We have no antibodies that are currently known to show blocking antibodies to VEGF-C. Please see this link to our IL-6 antibodies with blocking/neutralizing activity can be found at this link.

IL-6 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-16957

(1)

Citations (7)

Product Documents

100% Guarantee

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Predicted:

Rhesus Macaque (96%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot

Cited:

Immunohistochemistry-Paraffin, Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

View all IL-6 Primary Antibodies »

Product Specifications

Immunogen

Full length human IL-6 Recombinant protein.

Localization

Secreted

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for IL-6 Antibody - BSA Free

Western Blot: IL-6 Antibody [NBP2-16957]

Western Blot: IL-6 Antibody [NBP2-16957] - Human breast cancer cell line MDA-MB-231 was treated by Stat3 inhibitor and expression of IL-6 was detected by Western blot. Image from verified customer review.

Western Blot: IL-6 Antibody [NBP2-16957]

Western Blot: IL-6 Antibody [NBP2-16957] - Recombinant IL6 were separated by 12% SDS-PAGE, and the membrane was blotted with IL6 antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: IL-6 Antibody [NBP2-16957] -

Immunohistochemistry-Paraffin: IL-6 Antibody [NBP2-16957] - IL-6 antibody detects IL-6 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
IL-6 stained by IL-6 antibody (NBP2-16957) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: IL-6 Antibody [NBP2-16957] -

Untreated (-) and treated (+) THP-1 whole cell extract (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with IL6 antibody (NBP2-16957) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: IL-6 Antibody [NBP2-16957] -

Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with IL6 antibody (NBP2-16957) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

View 2 more images

Applications for IL-6 Antibody - BSA Free

Application

Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

Assay dependent

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Reviewed Applications

Read 1 review rated 3 using NBP2-16957 in the following applications:

Western Blot (1 Review)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: IL-6

This gene encodes a cytokine that functions in inflammation and the maturation of B cells. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. [provided by RefSeq]

Long Name

Interleukin 6

Alternate Names

BSF-2, BSF2, IFNB2, IL6, MGI-2A

Gene Symbol

IL6

UniProt

P05231

Additional IL-6 Products

Product Documents for IL-6 Antibody - BSA Free

Download Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for IL-6 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers

Conventional/Classical Dendritic Cells

Cytokines and Receptors in Allergy and Asthma

Cytokines and Receptors in Angiogenesis

Cytokines and Receptors Research

Diabetes

gamma delta T Cells

Glucose Homeostasis

Hypoxia Inducible Factors (HIFs)

IL-6 Family

Infectious Diseases

Inflammatory Mediators

Macrophage Activation Markers

Microglia Activation Markers

Myeloid-derived Suppressor Cells (MDSC)

Obesity

Plasmacytoid Dendritic Cells

T Cell Cytokine Signaling

Citations for IL-6 Antibody - BSA Free

Customer Reviews for IL-6 Antibody - BSA Free (1)

3 out of 5

1 Customer Rating

5 Stars

4 Stars

3 Stars

100%

2 Stars

1 Stars

Have you used IL-6 Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review

Customer Images

Showing 1 - 1 of 1 review Showing All

Filter By:

IL-6 Antibody

Name: Anonymous

Application: Western Blot

Sample Tested: Breast cancer cells

Species: Human

Verified Customer | Posted 09/21/2018

Human breast cancer cell line MDA-MB-231 was treated by Stat3 inhibitor and expression of IL-6 was detected by western blot.

There are no reviews that match your criteria.

Showing 1 - 1 of 1 review Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for IL-6 Antibody - BSA Free

Showing 1 - 1 of 1 FAQ Showing All

Q: I was wondering if you know of any blocking antibodies against IL-6 or VEGF-C that can be used in cell culture to quench these cytokines?

A:
We have no antibodies that are currently known to show blocking antibodies to VEGF-C. Please see this link to our IL-6 antibodies with blocking/neutralizing activity can be found at this link.