Mouse ADAM15 Ectodomain Antibody

(2 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse ADAM15 Ectodomain in direct ELISAs and Western blots. In Western blots (under reducing and non-reducing conditions), no cross-reactivity with the ectodomains of recombinant mouse (rm) ADAM9, rmADAM10, recombinant human (rh) ADAM8, rhADAM15, and rhADAM17 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse ADAM15
    Arg18-Ser694
    Accession # O88839
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse ADAM15
  • Immunohistochemistry
    5-15 µg/mL
    Perfusion fixed frozen sections of mouse brain (cerebellum)
  • Immunoprecipitation
    25 µg/mL
    Conditioned cell culture medium spiked with Recombinant Mouse ADAM15, see our available Western blot detection antibodies
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ADAM15

ADAM15, also known as MDC15 or metargidin, is a widely expressed metalloproteinase. It is a type I membrane protein that may be involved in cell adhesion and shedding of cell surface proteins.

  • Long Name:
    A Disintegrin and Metalloprotease-like Domain 15
  • Entrez Gene IDs:
    8751 (Human); 11490 (Mouse)
  • Alternate Names:
    ADAM 15; ADAM metallopeptidase domain 15; ADAM15; disintegrin-like, and cysteine-rich protein 15; EC 3.4.24; MDC15; MDC-15; Metargidin
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. An Adam15 amplification loop promotes vascular endothelial growth factor-induced ocular neovascularization.
    Authors: Xie B, Shen J, Dong A, Swaim M, Hackett SF, Wyder L, Worpenberg S, Barbieri S, Campochiaro PA
    FASEB J., 2008;22(8):2775-83.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: IP
  2. Coregulation of vascular tube stabilization by endothelial cell TIMP-2 and pericyte TIMP-3.
    Authors: Saunders WB, Bohnsack BL, Faske JB, Anthis NJ, Bayless KJ, Hirschi KK, Davis GE
    J. Cell Biol., 2006;175(1):179-91.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
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