Mouse Angiopoietin-like Protein 2/
ANGPTL2 Antibody

Catalog # Availability Size / Price Qty
AF1444
AF1444-SP

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Detection of Mouse Angiopoietin‑like Protein 2/ANGPTL2 by Western Blot.
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Citations (5)
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Mouse Angiopoietin-like Protein 2/
ANGPTL2 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Angiopoietin-like Protein 2/ANGPTL2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse (rm) ANGPTL6 and rmANGPTL1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse Angiopoietin-like 2
Pro23-His493
Accession # Q9R045
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
Immersion fixed frozen sections of mouse embryo (E15), skeletal muscle, and thymus

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse Angiopoietin-like Protein 2/ANGPTL2 antibody by Western Blot. View Larger

Detection of Mouse Angiopoietin‑like Protein 2/ANGPTL2 by Western Blot. Western blot shows lysates of mouse uterus tissue and MEF mouse embryonic feeder cells. PVDF membrane was probed with 2 µg/mL of Goat Anti-Mouse Angiopoietin-like Protein 2/ANGPTL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1444) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Angiopoietin-like Protein 2/ANGPTL2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Angiopoietin-like Protein 2/ANGPTL2

Angiopoietinlike2 (ANGPTL2), also known as ANGRP2, is a secreted glycoprotein structurally related to Angiopoietin. It does not bind the Tie1 or Tie2 receptors. ANGPTL2 induces sprouting but is not mitogenic in endothelial cells.

Entrez Gene IDs
23452 (Human); 26360 (Mouse)
Alternate Names
angiopoietin-like 2; Angiopoietin-like Protein 2; angiopoietin-related protein 2; ANGPTL2; ANGRP2; ARP2HARP; MGC8889

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Citations for Mouse Angiopoietin-like Protein 2/
ANGPTL2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Knockdown of ANGPTL2 promotes left ventricular systolic dysfunction by upregulation of NOX4 in mice
    Authors: Labbé, P;Martel, C;Shi, YF;Montezano, A;He, Y;Gillis, MA;Higgins, MÈ;Villeneuve, L;Touyz, R;Tardif, JC;Thorin-Trescases, N;Thorin, E;
    Frontiers in physiology
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  2. Angiopoietin-like 2 is essential to aortic valve development in mice
    Authors: Pauline Labbé, Victoria Munoz Goyette, Nathalie Thorin-Trescases, Louis Villeneuve, Ines Desanlis, Constance Delwarde et al.
    Communications Biology
  3. Cachexia-associated adipose loss induced by tumor-secreted leukemia inhibitory factor is counterbalanced by decreased leptin
    Authors: GK Arora, A Gupta, S Narayanan, T Guo, P Iyengar, RE Infante
    JCI Insight, 2018-07-26;3(14):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Dynamic roles of angiopoietin-like proteins 1, 2, 3, 4, 6 and 7 in the survival and enhancement of ex vivo expansion of bone-marrow hematopoietic stem cells
    Authors: Shahina Akhter, Md. Mashiar Rahman, Hyun Seo Lee, Hyeon-Jin Kim, Seong-Tshool Hong
    Protein & Cell
  5. Angiopoietin-like protein 2 mediates endotoxin-induced acute inflammation in the eye.
    Authors: Kanda A, Noda K, Oike Y, Ishida S
    Lab Invest, 2012-08-06;92(11):1553-63.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-P, Western Blot

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ANGPTL2 Antibody

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ANGPTL2 Antibody
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