Mouse B7-1/CD80 Antibody Summary
Accession # Q00609
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of B7‑1/CD80 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes, (A) treated with 200 ng/mL LPS for 16 hr, or (B) resting, were stained with Rabbit Anti-Mouse B7-1/CD80 Monoclonal Antibody (Catalog # MAB7401) followed by APC-conjugated Anti-Rabbit IgG Secondary Antibody (F0111) and Rat anti-Mouse B220 PE-conjugated Monoclonal Antibody (FAB1217P). Quadrant markers were set based on isotype control antibody (MAB1050, data not shown). Staining was done using our Staining Membrane-associated Proteins protocol.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
B7-1 and B7-2, together with their receptors CD28 and CTLA-4, constitute one of the dominant costimulatory pathways that regulate T and B cell responses. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20-100 fold higher affinity than CD28 and is involved in the down-regulation of the immune response. B7-1 is expressed on activated B cells, activated T cells, and macrophages. B7-2 is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells. Additionally, B7-2 is expressed at low levels on monocytes and can be up-regulated through interferon gamma. B7-1 and B7-2 are both members of the immunoglobulin superfamily. Mouse B7-1 is a 306 amino acid (aa) protein containing a putative 37 aa signal peptide, a 190 aa extracellular domain, a 22 aa transmembrane domain, and a 38 aa cytoplasmic domain. Mouse B7-1 and B7-2 share 28% amino acid identity. Mouse and human B7-1 share 44% amino acid identity. However, it has been observed that both human and mouse B7-1 and B7‑2 can bind to either human or mouse CD28 and CTLA-4, suggesting that there are conserved amino acids which form the B7-1/B7-2/CD28/CTLA-4 critical binding sites.
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- Freeman, G.J. et al. (1993) J. Exp. Med. 178:2185.
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