Mouse Bad N-Terminus Antibody

  (1 citations)     
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Product Details
Citations (1)
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  • Species Reactivity
    Mouse
  • Specificity
    Detects endogenous mouse Bad in Western blots. In Western blots, this antibody does not cross-react with human Bad. The antibody also detects a 32 kDa band in Western blots.
  • Source
    Monoclonal Rat IgG2A Clone # 64103
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant mouse Bad
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunocytochemistry
    5-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse Bad N-Terminus by Western Blot. Western blot shows lysates of CTLL‑2 mouse cytotoxic T cell line and L‑929 mouse fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse Bad N-Terminus Monoclonal Antibody (Catalog # MAB850) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Bad N-Terminus at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Immunocytochemistry
Bad in NIH‑3T3 Mouse Cell Line. Bad was detected in immersion fixed NIH‑3T3 mouse embryonic fibroblast cell line using Rat Anti-Mouse Bad N-Terminus Monoclonal Antibody (Catalog # MAB850) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Bad
Bcl-2 antagonist of cell death (Bad) is an 18 kDa cytoplasmic protein in the Bcl-2 family. It functions as a pro-apoptotic molecule by dimerizing with and inhibiting the anti-apoptotic proteins Bcl-2 and Bcl-xL. Prosurvival signals trigger the phosphorylation of Bad on Ser75, Ser99, and Ser115, disrupting its interaction with Bcl-2 and Bcl-xL and resulting in protection from apoptosis. Phosphorylation of Ser75 and Ser99 is also required for the ability of Bad to induce cell cycle arrrest in G1. Human Bad shares 75 % aa sequence identity with mouse and rat Bad.
  • Long Name:
    Bcl-xL/Bcl-2 Associated Death Promoter
  • Entrez Gene IDs:
    572 (Human); 12015 (Mouse); 64639 (Rat)
  • Alternate Names:
    Bad; BBC2; BBC6; bcl2 antagonist of cell death; BCL2-antagonist of cell death protein; BCL2-associated agonist of cell death; Bcl-2-binding component 6; BCL2-binding component 6; BCL2-binding protein; BCL2L8; Bcl2-L-8; BCL2L8bcl2-L-8; Bcl-2-like protein 8; BCL-X/BCL-2 binding protein; Bcl-XL/Bcl-2-associated death promoter
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Caspase-mediated Cleavage of Insulin Receptor Substrate.
    Authors: Green KA, Naylor MJ, Lowe ET, Wang P, Marshman E, Streuli CH
    J. Biol. Chem., 2004;279(24):25149-56.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB

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