Cathepsin H was detected in perfusion fixed frozen sections of mouse ovary using 1.7 µg/mL Goat Anti-Mouse Cathepsin H Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1013) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cathepsin H
Cathepsin H is a lysosomal cysteine protease of the papain family (1). It is synthesized as a precursor protein, consisting of a signal peptide (residues 1‑20), a propeptide (residues 21‑95), a mini chain (residues 96‑103), a heavy chain (residues 114‑290) and a light chain (residues 291‑333) (2, 3). A truncated form with a 12 amino acid deletion in the signal peptide region is secreted (4). Cathepsin H is the only known mono-aminopeptidase in the papain family (5). Cathepsin H expression is significantly increased in disease states such as in prostate tumors, sera of asthmatic patients, and mucosa of colorectal cancer patients (4, 6, 7).
Kirschke, H. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) p. 1089, Academic Press, San Diego.
Lafuse, W.P. et al. (1995) J. Leukoc. Biol. 57:663.
Soderstrom, M. et al. (1999) Biochim. Biophys. Acta 1446:35.
Waghray, A. et al. (2002) J. Biol. Chem. 277:11533.
Guncar, G. et al. (1998) Structure 6:51.
Cimerman, N. et al. (2001) Clin. Chim. Acta 310:113.
del Re, E.C. et al. (2000) Br. J. Cancer. 82:1317.
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