Mouse CCL6/C10 Antibody

Catalog # Availability Size / Price Qty
AB-487-NA
Chemotaxis Induced by CCL6/C10 and Neutralization by Mouse CCL6/C10 Antibody.
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Mouse CCL6/C10 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CCL6/C10 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Protein A or G purified
Immunogen
E. coli-derived recombinant mouse CCL6/C10
Gly22-Ala116
Accession # P27784
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse CCL6/C10 (Catalog # 487-C)
Neutralization
Measured by its ability to neutralize CCL6/C10-induced chemotaxis in human monocytes. The Neutralization Dose (ND50) is typically 70-100 µg/mL in the presence of 0.5 µg/mL Recombinant Mouse CCL6/C10.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization Chemotaxis Induced by CCL6/C10 and Neutralization by Mouse CCL6/C10 Antibody. View Larger

Chemotaxis Induced by CCL6/C10 and Neutralization by Mouse CCL6/C10 Antibody. Recombinant Mouse CCL6/C10 (Catalog # 487-C) chemoattracts human monocytes in a dose-dependent manner (orange line). The amount of cells that migrated through the filter was measured by LeukoStat™ staining (Fisher Scientific). Chemotaxis elicited by Recombinant Mouse CCL6/C10 (0.5 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL6/C10 Polyclonal Antibody (Catalog # AB-487-NA). The ND50 is typically 70-100 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 1 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL6/C10

Mouse CCL6, a member of the beta subfamily of chemokines, was initially identified as a transcript that is induced in bone marrow cells upon stimulation with GM-CSF. A human homologue for mouse CCL6 has not been identified. The mCCL6 cDNA encodes a 116 amino acid residue precursor protein with a hydrophobic signal peptide that is cleaved to yield a 95 amino acid residue mature protein. Compared to other chemokines, mCCL6 has a large N-terminal extension. The expression of CCL6 mRNA has been detected in mouse monocytes and neutrophils, where it is strongly induced upon GM-CSF stimulation. CCL6 expression has also been detected in an IL-2-dependent T cell line, where the expression is inhibited following T cell stimulation. Mouse CCL6 expression was also shown to be strongly induced by IL-4 in mouse macrophages. The gene for CCL6 has been mapped to mouse chromosome 11.

Entrez Gene IDs
20305 (Mouse); 287910 (Rat)
Alternate Names
C10; CCL6; MRP-1; SCYA6

Product Datasheets

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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