Chemotaxis Induced by CCL6/C10 and Neutralization by Mouse CCL6/C10 Antibody. |
Recombinant Mouse CCL6/C10 (Catalog # 487-C) chemoattracts human monocytes in a dose-dependent manner (orange line). The amount of cells that migrated through the filter was measured by LeukoStat™ staining (Fisher Scientific). Chemotaxis elicited by Recombinant Mouse CCL6/C10 (0.5 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL6/C10 Polyclonal Antibody (Catalog # AB‑487‑NA). The ND50 is typically 70‑100 µg/mL.
Mouse CCL6, a member of the beta subfamily of chemokines, was initially identified as a transcript that is induced in bone marrow cells upon stimulation with GM-CSF. A human homologue for mouse CCL6 has not been identified. The mCCL6 cDNA encodes a 116 amino acid residue precursor protein with a hydrophobic signal peptide that is cleaved to yield a 95 amino acid residue mature protein. Compared to other chemokines, mCCL6 has a large N-terminal extension. The expression of CCL6 mRNA has been detected in mouse monocytes and neutrophils, where it is strongly induced upon GM-CSF stimulation. CCL6 expression has also been detected in an IL-2-dependent T cell line, where the expression is inhibited following T cell stimulation. Mouse CCL6 expression was also shown to be strongly induced by IL-4 in mouse macrophages. The gene for CCL6 has been mapped to mouse chromosome 11.