Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, Adhesion Blockade, Flow Cytometry, Immunocytochemistry, CyTOF-ready

Cited:

Western Blot, Flow Cytometry, ELISA Development (Capture)

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all CD83 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CD83
Met22-Ala134
Accession # O88324

Specificity

Detects mouse CD83 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse CD83 Antibody

CD83 antibody in Mouse Splenocytes by Immunocytochemistry (ICC).

CD83 in Mouse Splenocytes.

CD83 was detected in immersion fixed mouse splenocytes using 10 µg/mL Goat Anti-Mouse CD83 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1437) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

CD83 antibody in Mouse Dendritic Cells by Immunocytochemistry (ICC).

CD83 in Mouse Dendritic Cells.

CD83 was detected in immersion fixed LPS-stimulated mouse dendritic cells using Goat Anti-Mouse CD83 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1437) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

CD83 in Mouse Spleen Tissue.

CD83 in Mouse Spleen Tissue.

Dilution: 1:500. AGR with Tris/EDTA pH 9.0 for 10min. Serum blocking with normal rabbit serum (5% for 1hour). anti-CD83 incubation for 16h at 4°C overnight. Secondary antibody: rabbit anti goat IgG Biotin. DAB. Image from a verified customer review.
Detection of CD83 by Western Blot

Detection of CD83 by Western Blot

Analyses of CD83 deficient murine Mφ. Mφ were generated from CD83wt or CD83 cKO mice and subsequently stimulated with IFN-gamma or IL-4 for 16h or left untreated. (A)Cd83 expression levels were determined by qPCR and normalized to CD83wt BMDMs (n = 10). (B) Assessment of CD83 expression levels by flow cytometry (n = 20). (C) Assessment of knock-out efficiency in whole cell lysates from mock-, IFN-gamma or IL-4 stimulated Mφ by Western blotting. beta -actin served as a loading control. See full uncut gels in Supplemental Material (S1)(D) Cell viability assessment using flow cytometry (n = 24). (E) Differentiation efficacy assessing the percentage of F4/80+CD11b+ cells by flow cytometry, representing the Mφ population (n ≥ 24). (F) Expression levels of F4/80 and CD11b within the Mφ population (n ≥ 40). The gating strategy for the Mφ population is depicted in Supplementary Figure 2. Statistical analyses were performed by One-way ANOVA or the appropriate corresponding non-parametric test. Data are represented as mean ± SEM. Experiments were performed at least three times. ***p<0.001; **** p< 0.0001. The absence of asterisks indicates that there is no statistical significance. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36875129), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of CD83 by Western Blot

Detection of CD83 by Western Blot

Analyses of CD83 deficient murine Mφ. Mφ were generated from CD83wt or CD83 cKO mice and subsequently stimulated with IFN-gamma or IL-4 for 16h or left untreated. (A)Cd83 expression levels were determined by qPCR and normalized to CD83wt BMDMs (n = 10). (B) Assessment of CD83 expression levels by flow cytometry (n = 20). (C) Assessment of knock-out efficiency in whole cell lysates from mock-, IFN-gamma or IL-4 stimulated Mφ by Western blotting. beta -actin served as a loading control. See full uncut gels in Supplemental Material (S1)(D) Cell viability assessment using flow cytometry (n = 24). (E) Differentiation efficacy assessing the percentage of F4/80+CD11b+ cells by flow cytometry, representing the Mφ population (n ≥ 24). (F) Expression levels of F4/80 and CD11b within the Mφ population (n ≥ 40). The gating strategy for the Mφ population is depicted in Supplementary Figure 2. Statistical analyses were performed by One-way ANOVA or the appropriate corresponding non-parametric test. Data are represented as mean ± SEM. Experiments were performed at least three times. ***p<0.001; **** p< 0.0001. The absence of asterisks indicates that there is no statistical significance. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36875129), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse CD83 Antibody

Application
Recommended Usage

Adhesion Blockade

The adhesion of human monocyte-derived dendritic cells (5 x 104 cells/well) to immobilized Recombinant Mouse CD83 Fc Chimera (Catalog # 1437-CD, 2.5 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 40 µg/mL of the antibody.

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: LPS-treated mature mouse dendritic cells

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed mouse splenocytes and dendritic cells

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse CD83 Fc Chimera (Catalog # 1437-CD)

Reviewed Applications

Read 2 reviews rated 5 using AF1437 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD83

Mouse CD83 is a 30‑35 kDa member of the Siglec (or sialic-acid-binding immunoglobulin-like lectin) family of transmembrane proteins (1, 2, 3). CD83 is synthesized as a type I transmembrane glycoprotein that contains a 114 amino acid (aa) extracellular region, a 22 aa transmembrane segment, and a 39 aa cytoplasmic domain. It contains one V type Ig-like domain in the extracellular region with no inhibitory cytoplasmic motif(s). In the extracellular region, mouse and human CD83 are 66% aa identical (1, 2, 4). Relative to mouse, human CD83 has an 11 aa insertion in its extracellular domain and is expressed as a 45‑55 kDa protein (1, 4, 5, 6). No alternate splice variants have been reported for mouse. In human, however, one soluble splice form has been reported and proteolytic processing is suggested to generate a second circulating isoform (6, 7). Notably, although soluble CD83 has the potential to exist as either a monomer or disulfide-linked dimer, both show immunosuppressive activity (4, 8, 9). Membrane CD83, by contrast, is immunostimulatory (10). CD83 is a primary marker for dendritic cells (3, 5, 6). It is also found on B cells (6, 11), neutrophils (12), monocytes and macrophages (13). Except for dendritic cells, CD83 expression is often transient. CD83 binds to sialic acids on monocytes (3). The function of CD83 is only now becoming clear. As noted, membrane-immobilized CD83 appears to promote T cell proliferation, particularly of CD8+ cytotoxic T cells (14). On monocytes, CD83 may also drive monocytes into a fibrocyte phenotype (14). And a lack of membrane-expressed CD83 leads to an unusual IL-4/IL-10 producing CD4+ T cell phenotype (15).

References

  1. Berchtold, S. et. al. (1999) FEBS Lett. 461:211.
  2. Fujimoto, Y. and T.F. Tedder (2006) J. Med. Dent. Sci. 53:85.
  3. Scholler, N. et. al. (2001) J. Immunol. 166:3865.
  4. Lechmann, M. et al. (2005) Biochem. Biophys. Res. Commun. 329:132.
  5. Zhou, L-J. et. al. (1992) J. Immunol. 149:735.
  6. Hock, B.D. et al. (2001) Int. Immunol. 13:959.
  7. Dudziak, D. et al. (2005) J. Immunol. 174:6672.
  8. Kotzor, N. et al. (2004) Immunobiology 209:129.
  9. Zinser, E. et al. (2006) Immunobiology 21:449.
  10. Hirano, N. et al. (2006) Blood 107:1528.
  11. Cramer, S.O. et al. (2000) Int. Immunol. 12:1347.
  12. Yamashiro, S. et al. (2000) Blood 96:3958.
  13. Cao, W. et al. (2005) Biochem. J. 385:85.
  14. Scholler, N. et al. (2002) J. Immunol. 168:2599.
  15. Garcia-Martinez, L.F. et al. (2004) J. Immunol. 173:2995.

Alternate Names

BL11, CD83, HB15

Entrez Gene IDs

9308 (Human); 12522 (Mouse)

Gene Symbol

CD83

UniProt

O88324

Additional CD83 Products

Product Documents for Mouse CD83 Antibody

Certificate of Analysis

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Product Specific Notices for Mouse CD83 Antibody

For research use only

Citations for Mouse CD83 Antibody

Customer Reviews for Mouse CD83 Antibody (2)

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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • Mouse CD83 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Spleen tissue
    Species: Mouse
    Verified Customer | Posted 08/18/2025
    Dilution: 1:500. AGR with Tris/EDTA pH 9.0 for 10min. Serum blocking with normal rabbit serum (5% for 1hour). anti-CD83 incubation for 16h at 4°C overnight. Secondary antibody: rabbit anti goat IgG Biotin. DAB.
    Mouse CD83 Antibody AF1437
    Bio-Techne Response
    This review reflects a new species or application tested on a primary antibody.
  • Mouse CD83 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Bone marrow-derived macrophages
    Species: Mouse
    Verified Customer | Posted 09/30/2020
    Bone marrow-derived macrophages from wt or conditional KO mice were stimulated either with IFN-y or IL-4 for 16 h. Whole cell lysates were prepared and 50 µg of protein was used for SDS-PAGE (16%) and western blotting (primary anti-CD83 1:500; secondary anti-goat-HRP 1:7500). Specific signal only in WT-macrophages, no signal in KO cells. One unspecific band at approx. 65 kDa (see arrow-head).
    Mouse CD83 Antibody AF1437

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