Mouse CD83 Antibody

Catalog # Availability Size / Price Qty
AF1437
AF1437-SP
CD83 in Mouse Splenocytes.
2 Images
Product Details
Citations (3)
FAQs
Supplemental Products
Reviews (1)

Mouse CD83 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CD83 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD83
Met22-Ala134
Accession # O88324
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse CD83 Fc Chimera (Catalog # 1437-CD)
Flow Cytometry
2.5 µg/106 cells
LPS-treated mature mouse dendritic cells
Adhesion Blockade
The adhesion of human monocyte-derived dendritic cells (5 x 104 cells/well) to immobilized Recombinant Mouse CD83 Fc Chimera (Catalog # 1437-CD, 2.5 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 40 µg/mL of the antibody.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry CD83 antibody in Mouse Splenocytes by Immunocytochemistry (ICC). View Larger

CD83 in Mouse Splenocytes. CD83 was detected in immersion fixed mouse splenocytes using 10 µg/mL Goat Anti-Mouse CD83 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1437) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunocytochemistry CD83 antibody in Mouse Dendritic Cells by Immunocytochemistry (ICC). View Larger

CD83 in Mouse Dendritic Cells. CD83 was detected in immersion fixed LPS-stimulated mouse dendritic cells using Goat Anti-Mouse CD83 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1437) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD83

Mouse CD83 is a 30‑35 kDa member of the Siglec (or sialic-acid-binding immunoglobulin-like lectin) family of transmembrane proteins (1, 2, 3). CD83 is synthesized as a type I transmembrane glycoprotein that contains a 114 amino acid (aa) extracellular region, a 22 aa transmembrane segment, and a 39 aa cytoplasmic domain. It contains one V type Ig-like domain in the extracellular region with no inhibitory cytoplasmic motif(s). In the extracellular region, mouse and human CD83 are 66% aa identical (1, 2, 4). Relative to mouse, human CD83 has an 11 aa insertion in its extracellular domain and is expressed as a 45‑55 kDa protein (1, 4, 5, 6). No alternate splice variants have been reported for mouse. In human, however, one soluble splice form has been reported and proteolytic processing is suggested to generate a second circulating isoform (6, 7). Notably, although soluble CD83 has the potential to exist as either a monomer or disulfide-linked dimer, both show immunosuppressive activity (4, 8, 9). Membrane CD83, by contrast, is immunostimulatory (10). CD83 is a primary marker for dendritic cells (3, 5, 6). It is also found on B cells (6, 11), neutrophils (12), monocytes and macrophages (13). Except for dendritic cells, CD83 expression is often transient. CD83 binds to sialic acids on monocytes (3). The function of CD83 is only now becoming clear. As noted, membrane-immobilized CD83 appears to promote T cell proliferation, particularly of CD8+ cytotoxic T cells (14). On monocytes, CD83 may also drive monocytes into a fibrocyte phenotype (14). And a lack of membrane-expressed CD83 leads to an unusual IL-4/IL-10 producing CD4+ T cell phenotype (15).

References
  1. Berchtold, S. et. al. (1999) FEBS Lett. 461:211.
  2. Fujimoto, Y. and T.F. Tedder (2006) J. Med. Dent. Sci. 53:85.
  3. Scholler, N. et. al. (2001) J. Immunol. 166:3865.
  4. Lechmann, M. et al. (2005) Biochem. Biophys. Res. Commun. 329:132.
  5. Zhou, L-J. et. al. (1992) J. Immunol. 149:735.
  6. Hock, B.D. et al. (2001) Int. Immunol. 13:959.
  7. Dudziak, D. et al. (2005) J. Immunol. 174:6672.
  8. Kotzor, N. et al. (2004) Immunobiology 209:129.
  9. Zinser, E. et al. (2006) Immunobiology 21:449.
  10. Hirano, N. et al. (2006) Blood 107:1528.
  11. Cramer, S.O. et al. (2000) Int. Immunol. 12:1347.
  12. Yamashiro, S. et al. (2000) Blood 96:3958.
  13. Cao, W. et al. (2005) Biochem. J. 385:85.
  14. Scholler, N. et al. (2002) J. Immunol. 168:2599.
  15. Garcia-Martinez, L.F. et al. (2004) J. Immunol. 173:2995.
Entrez Gene IDs
9308 (Human); 12522 (Mouse)
Alternate Names
B-cell activation protein; BL11; BL11CD83 antigen; CD83 antigen (activated B lymphocytes, immunoglobulin superfamily); CD83 molecule; CD83; Cell surface protein HB15; cell-surface glycoprotein; HB15; HB15hCD83

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Citations for Mouse CD83 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Dendritic cell CD83 homotypic interactions regulate inflammation and promote mucosal homeostasis.
    Authors: Bates J, Flanagan K, Mo L, Ota N, Ding J, Ho S, Liu S, Roose-Girma M, Warming S, Diehl L
    Mucosal Immunol, 2014-09-10;8(2):414-28.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Capture)
  2. Inactivated Sendai virus particles eradicate tumors by inducing immune responses through blocking regulatory T cells.
    Authors: Kurooka M, Kaneda Y
    Cancer Res., 2007-01-01;67(1):227-36.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. CD83 expressed by macrophages is an important immune checkpoint molecule for the resolution of inflammation
    Authors: Katrin Peckert-Maier, Pia Langguth, Astrid Strack, Lena Stich, Petra Mühl-Zürbes, Christine Kuhnt et al.
    Frontiers in Immunology

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Mouse CD83 Antibody
By Anonymous on 09/30/2020
Application: WB Sample Tested: Bone marrow-derived macrophages Species: Mouse

Bone marrow-derived macrophages from wt or conditional KO mice were stimulated either with IFN-y or IL-4 for 16 h. Whole cell lysates were prepared and 50 µg of protein was used for SDS-PAGE (16%) and western blotting (primary anti-CD83 1:500; secondary anti-goat-HRP 1:7500).
Specific signal only in WT-macrophages, no signal in KO cells.
One unspecific band at approx. 65 kDa (see arrow-head).