Mouse CD83 Antibody

(2 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CD83 in direct ELISAs and Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse CD83
    Met22-Ala134
    Accession # O88324
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse CD83 Fc Chimera (Catalog # 1437-CD)
  • Flow Cytometry
    2.5 µg/106 cells
    LPS-treated mature mouse dendritic cells
  • Adhesion Blockade
    The adhesion of human monocyte-derived dendritic cells (5 x 104 cells/well) to immobilized Recombinant Mouse CD83 Fc Chimera (Catalog # 1437-CD, 2.5 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 40 µg/mL of the antibody.
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
CD83 in Mouse Splenocytes. CD83 was detected in immersion fixed mouse splenocytes using 10 µg/mL Goat Anti-Mouse CD83 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1437) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Immunocytochemistry
CD83 in Mouse Dendritic Cells. CD83 was detected in immersion fixed LPS-stimulated mouse dendritic cells using Goat Anti-Mouse CD83 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1437) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD83

Mouse CD83 is a 30‑35 kDa member of the Siglec (or sialic-acid-binding immunoglobulin-like lectin) family of transmembrane proteins (1, 2, 3). CD83 is synthesized as a type I transmembrane glycoprotein that contains a 114 amino acid (aa) extracellular region, a 22 aa transmembrane segment, and a 39 aa cytoplasmic domain. It contains one V type Ig-like domain in the extracellular region with no inhibitory cytoplasmic motif(s). In the extracellular region, mouse and human CD83 are 66% aa identical (1, 2, 4). Relative to mouse, human CD83 has an 11 aa insertion in its extracellular domain and is expressed as a 45‑55 kDa protein (1, 4, 5, 6). No alternate splice variants have been reported for mouse. In human, however, one soluble splice form has been reported and proteolytic processing is suggested to generate a second circulating isoform (6, 7). Notably, although soluble CD83 has the potential to exist as either a monomer or disulfide-linked dimer, both show immunosuppressive activity (4, 8, 9). Membrane CD83, by contrast, is immunostimulatory (10). CD83 is a primary marker for dendritic cells (3, 5, 6). It is also found on B cells (6, 11), neutrophils (12), monocytes and macrophages (13). Except for dendritic cells, CD83 expression is often transient. CD83 binds to sialic acids on monocytes (3). The function of CD83 is only now becoming clear. As noted, membrane-immobilized CD83 appears to promote T cell proliferation, particularly of CD8+ cytotoxic T cells (14). On monocytes, CD83 may also drive monocytes into a fibrocyte phenotype (14). And a lack of membrane-expressed CD83 leads to an unusual IL-4/IL-10 producing CD4+ T cell phenotype (15).

  • References:
    1. Berchtold, S. et. al. (1999) FEBS Lett. 461:211.
    2. Fujimoto, Y. and T.F. Tedder (2006) J. Med. Dent. Sci. 53:85.
    3. Scholler, N. et. al. (2001) J. Immunol. 166:3865.
    4. Lechmann, M. et al. (2005) Biochem. Biophys. Res. Commun. 329:132.
    5. Zhou, L-J. et. al. (1992) J. Immunol. 149:735.
    6. Hock, B.D. et al. (2001) Int. Immunol. 13:959.
    7. Dudziak, D. et al. (2005) J. Immunol. 174:6672.
    8. Kotzor, N. et al. (2004) Immunobiology 209:129.
    9. Zinser, E. et al. (2006) Immunobiology 21:449.
    10. Hirano, N. et al. (2006) Blood 107:1528.
    11. Cramer, S.O. et al. (2000) Int. Immunol. 12:1347.
    12. Yamashiro, S. et al. (2000) Blood 96:3958.
    13. Cao, W. et al. (2005) Biochem. J. 385:85.
    14. Scholler, N. et al. (2002) J. Immunol. 168:2599.
    15. Garcia-Martinez, L.F. et al. (2004) J. Immunol. 173:2995.
  • Entrez Gene IDs:
    9308 (Human); 12522 (Mouse)
  • Alternate Names:
    B-cell activation protein; BL11; BL11CD83 antigen; CD83 antigen (activated B lymphocytes, immunoglobulin superfamily); CD83 molecule; CD83; Cell surface protein HB15; cell-surface glycoprotein; HB15; HB15hCD83
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Species
Applications
Sample Type
  1. Dendritic cell CD83 homotypic interactions regulate inflammation and promote mucosal homeostasis.
    Authors: Bates J, Flanagan K, Mo L, Ota N, Ding J, Ho S, Liu S, Roose-Girma M, Warming S, Diehl L
    Mucosal Immunol, 2015;8(2):414-28.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: ELISA Capture
  2. Inactivated Sendai virus particles eradicate tumors by inducing immune responses through blocking regulatory T cells.
    Authors: Kurooka M, Kaneda Y
    Cancer Res., 2007;67(1):227-36.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
Cell and Tissue Staining Kits
Description Application Cat# Citations Images  

Anti-Goat HRP-DAB Cell & Tissue Staining Kit

CTS008 13
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Secondary Antibodies
Description Application Cat# Citations Images  

Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG HRP-conjugated Antibody

WB HAF017 15  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 9
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG Antibody

WB AF109 6
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG VisUCyte HRP Polymer

IHC VC004  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Mouse CD83 Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies

Feedback

Customer Information