In a functional ELISA, 1-4 µg/mL of this antibody will block 50% of the binding of 100 ng/mL of biotinylated AGE‑BSA to immobilized Recombinant Mouse CL-P1/COLEC12 (Catalog # 3130-CL) coated at 5 µg/mL (100 µL/well). At 100 μg/mL, this antibody will block >90% of the binding.
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of CL‑P1/COLEC12 in J774A.1 Mouse Cell Line by Flow Cytometry.
J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Goat Anti-Mouse CL‑P1/COLEC12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3130, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Collectins are a family of Ca++-dependent, C-type lectins that contain a collagenous domain and function as recognition molecules for molecular patterns found on pathogens (1‑4). Collectin placenta 1 (CL-P1), also known as collectin sub-family member 12 and scavenger receptor with C-type lectin type I (SRCL), is a 140 kDa member of the collectin family of glycoproteins. With two exceptions, all collectins are secreted. CL-P1 is the only collectin known to be membrane bound, while CL‑L1 (collectin liver-1) is the only known cytoplasmic collectin (1). Mouse CL-P1 is synthesized as a 742 amino acid (aa) type II transmembrane glycoprotein that includes an N-terminal 39 aa cytoplasmic domain, an 18 aa transmembrane segment, and a 685 aa C-terminal extracellular domain. The short cytoplasmic domain contains an internalization motif (Y-K-R-F), while the ECD is complex, demonstrating a coiled-coil segment, a Ser-Thr rich region, a collagen-like structure, and a C‑type lectin/carbohydrate recognition domain (CRD) (5, 6). Unlike human CL-P1, no splice variants of mouse CL-P1 have been described (5, 7). Trimerization of CL‑P1 is mediated by its collagen-like and coiled-coil helical domains (1, 6). Within the ECD, mouse CL-P1 shares 88%, 89%, 92%, and 98% aa sequence identity with bovine, canine, human, and rat CL-P1, respectively. The CRD shares 23‑27% aa sequence identity with the CRD of collectins CL-L1, collectin sub-family member 11, MBL, SP-A1, and SP-D. Notably, this CRD recognizes galactose and fucose within the context of asialo-orosomucoids associated with the Lewisx epitope (8, 9). CL‑P1 is expressed in vascular endothelial cells and may play a role in bacterial recognition or as a scavenger receptor for desialylated glycoproteins (6, 8).
van de Wetering, J.K. et al. (2004) Eur. J. Biochem. 271:1229.
Holmskov, U. et al. (2003) Annu. Rev. Immunol. 21:547.
Hoppe, H-J. and K. Reid (1994) Protein Sci. 3:1143.
Hickling, T.P. et al. (2004) J. Leukoc. Biol. 75:27.
Nakamura, K. et al. (2001) Biochim. Biophys. Acta 1522:53.
Ohtani, K. et al. (2001) J. Biol. Chem. 276:44222.
Nakamura, K. et al. (2001) Biochem. Biophys. Res. Commun. 280:1028.
Coombs, P.J. et al. (2005) J. Biol. Chem. 280:22993.
Yoshida, T. et al. (2003) J. Biochem. 133:271.
Collectin Placenta 1
Entrez Gene IDs:
81035 (Human); 140792 (Mouse); 361289 (Rat)
CL-P1; CLP1, cleavage and polyadenylation factor I subunit, homolog (S. cerevisiae); COLEC12; EC 184.108.40.206; hClp1; HEABATP/GTP-binding protein; homolog of yeast CFIA subunit Clp1p; NSR2; Polynucleotide kinase Clp1; polyribonucleotide 5'-hydroxyl-kinase Clp1; Pre-mRNA cleavage complex II protein Clp1; SCARA4; SRCL Type I
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The data collected includes not only links to publications in PubMed,
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