Mouse CREG Antibody

Detection of CREG by Western Blot CREG1 abundance is post-translationally modulated by CTSB inhibition and CTSB induction. a The abundance of CREG1 is increased in PyMT cells treated with the broad spectrum CTSB inhibitor E64d (10 µM). b CREG1 abundance can be reduced upon doxycycline induction (1 µM) of CTSB in a PyMT Ctsb−/− cell line carrying a CTSB doxycycline-inducible vector. c No significant changes of CREG1 at the mRNA level (qRT-PCR) were observed in tumor-cell co-cultures carrying Mϕ lacking both Ctsb and Ctsz compared to wild-type Mϕ (dashed line). d Inhibition of CTSB in PyMT cells with E64d (10 µM) or with CA-074 (10 µM) lead to no significant changes of CREG1 at the mRNA level compared to control (dashed line). n.s. non-significant change, Mϕ macrophages, indCTSB doxycycline-inducible CTSB, DOX doxycycline, CTSB human CTSB Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32385587), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of CREG by Western Blot CREG1 is partially processed by CTSB generating a neo N-termini. a Partial processing of rCREG1 (2 µg) is observed by incubation with rCTSB (200 ng) after 6 h and increased after 24 h at pH 5.0. Incubation of rCREG1 with rCTSZ does not have significant changes; whereas, incubation of rCREG1 with both rCTSB and rCTSZ leads to a moderate increase in the processing at 6 and 24 h. At pH 6.6, no changes are observed with neither rCTSB, rCTSZ, nor with both CTSB and CTSZ. b N-terminal sequencing (Edman degradation) done with samples treated or not with rCTSB or rCTSB and rCTSZ after 24 h identified a potential CTSB cleavage site (Arrow H37–G38 bond). The sequence R32GGRD (blue) was identified in non-treated rCREG1 and the same neo N-termini GDWDV (red) was identified in samples treated with rCTSB or rCTSB and rCTSZ. Signal peptide (dark yellow); rCREG1 recombinant murine CREG1, rCTSB recombinant murine CTSB, rCTSZ recombinant murine CTSZ Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32385587), licensed under a CC-BY license. Not internally tested by R&D Systems.